PHF11 (NM_001040444) Human Tagged ORF Clone

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SKU
RC214020
PHF11 (Myc-DDK-tagged)-Human PHD finger protein 11 (PHF11), transcript variant 2
  $300.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol PHF11
Synonyms APY; BCAP; IGEL; IGER; IGHER; NY-REN-34; NYREN34
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC214020 representing NM_001040444
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAAAAAAGGACATGTGCACTCTGCCCCAAAGATGTCGAATATAATGTCCTGTACTTTGCACAATCAG
AGAATATAGCTGCTCATGAGAATTGTTTGCTGTATTCTTCAGGACTTGTGGAATGTGAGGATCAGGATCC
ACTTAATCCTGATAGAAGTTTTGATGTGGAATCAGTAAAGAAAGAAATCCAGAGAGGAAGGAAGTTGAAA
TGCAAATTTTGTCATAAAAGAGGAGCCACCGTGGGATGTGATTTAAAAAACTGTAACAAGAATTACCACT
TTTTCTGTGCCAAGAAGGACGACGCAGTTCCACAGTCTGATGGAGTTCGAGGAATTTATAAACTGCTTTG
CCAGCAACATGCTCAATTCCCGATCATCGCTCAAAGTGCTAAATTTTCAGGAGTGAAAAGAAAAAGAGGA
AGGAAGAAACCCCTCTCAGGCAATCATGTACAGCCACCCGAAACAATGAAATGTAATACATTCATAAGAC
AAGTGAAAGAAGAGCATGGCAGACACACAGATGCAACTGTGAAAGTTCCTTTTCTTAAGAAATGCAAGGA
AGCAGGACTTCTTAATTACTTACTTGAAGAAATATTAGACAAAGTTCATTCAATTCCAGAAAAACTCATG
GATGAGACTACTTCAGAATCAGACTATGAAGAAATCGGGAGTGCACTTTTTGACTGTAGATTGTTCGAAG
ACACATTTGTAAATTTTCAAGCAGCAATAGAGAAAAAAATTCATGCATCTCAACAAAGGTGGCAGCAGTT
GAAGGAAGAGATTGAGCTACTTCAGGACTTAAAACAAACCTTGTGCTCTTTTCAAGAAAATAGAGATCTT
ATGTCAAGTTCTACATCAATATCATCCCTGTCTTAT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC214020 representing NM_001040444
Red=Cloning site Green=Tags(s)

MEKRTCALCPKDVEYNVLYFAQSENIAAHENCLLYSSGLVECEDQDPLNPDRSFDVESVKKEIQRGRKLK
CKFCHKRGATVGCDLKNCNKNYHFFCAKKDDAVPQSDGVRGIYKLLCQQHAQFPIIAQSAKFSGVKRKRG
RKKPLSGNHVQPPETMKCNTFIRQVKEEHGRHTDATVKVPFLKKCKEAGLLNYLLEEILDKVHSIPEKLM
DETTSESDYEEIGSALFDCRLFEDTFVNFQAAIEKKIHASQQRWQQLKEEIELLQDLKQTLCSFQENRDL
MSSSTSISSLSY

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001040444
ORF Size 876 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001040444.2, NP_001035534.1
RefSeq Size 1471 bp
RefSeq ORF 879 bp
Locus ID 51131
UniProt ID Q9UIL8
Cytogenetics 13q14.2
Protein Families Druggable Genome, Transcription Factors
MW 33.3 kDa
Summary This gene encodes a protein containing a PHD (plant homeodomain) type zinc finger. This gene has been identified in some studies as a candidate gene for asthma. Naturally-occurring readthrough transcription may occur from the upstream SETDB2 (SET domain bifurcated 2) gene to this locus. Alternative splicing results in multiple transcript variants. provided by RefSeq, Mar 2016
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Documents
Resources
"NM_001040444" in other vectors (4)
SKU Description Size Price
RC214020L3 Lenti ORF clone of Human PHD finger protein 11 (PHF11), transcript variant 2, Myc-DDK-tagged 10 ug
$600.00
RC214020L4 Lenti ORF clone of Human PHD finger protein 11 (PHF11), transcript variant 2, mGFP tagged 10 ug
$600.00
RG214020 PHF11 (tGFP-tagged) - Human PHD finger protein 11 (PHF11), transcript variant 2 10 ug
$500.00
SC310740 PHF11 (untagged)-Human PHD finger protein 11 (PHF11), transcript variant 2 10 ug
$330.00

Plasmid Map for RC214020

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Cloning scheme for RC214020

Circular map for RC214020

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