DPP6 (NM_130797) Human Tagged ORF Clone

  • TrueORF®
SKU
RC214014
DPP6 (Myc-DDK-tagged)-Human dipeptidyl-peptidase 6 (DPP6), transcript variant 1
  $1,188.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol DPP6
Synonyms DPL1; DPPX; MRD33; VF2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC214014 representing NM_130797
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTTCGCTGTACCAGAGGTTCACTGGCAAGATCAACACCTCGAGGTCCTTCCCCGCGCCCCCGGAGG
CGAGTCACCTCCTGGGCGGCCAGGGGCCCGAGGAGGACGGCGGCGCAGGAGCCAAGCCCCTCGGCCCGCG
GGCGCAGGCGGCGGCGCCCCGGGAGCGCGGCGGCGGCGGCGGCGGCGCGGGTGGCCGGCCCCGGTTCCAG
TACCAGGCGCGGAGCGATGGTGACGAGGAGGACGAGCTGGTGGGGAGTAACCCTCCGCAGAGGAATTGGA
AAGGAATAGCAATTGCACTGCTTGTCATTCTGGTCATCTGCTCCTTGATCGTCACCTCGGTCATACTTCT
GACACCAGCGGAAGATAATAGTCTGTCTCAAAAGAAGAAGGTCACTGTAGAAGATCTCTTCAGTGAAGAC
TTCAAAATTCATGACCCCGAGGCTAAGTGGATAAGTGATACAGAATTCATCTACAGAGAACAGAAAGGAA
CAGTGAGACTGTGGAATGTTGAAACAAATACTTCTACTGTCTTAATAGAAGGCAAAAAAATTGAATCATT
AAGAGCCATCAGATATGAAATATCTCCAGATAGAGAGTATGCACTTTTTTCATACAATGTGGAACCCATA
TATCAACACTCGTATACTGGATATTACGTCCTGAGCAAAATTCCTCATGGGGATCCTCAAAGTCTGGACC
CACCAGAAGTCAGCAATGCAAAACTTCAGTATGCAGGATGGGGCCCTAAAGGCCAACAGCTGATATTTAT
TTTTGAAAACAATATCTACTACTGTGCACATGTCGGGAAACAGGCCATCCGTGTGGTCTCCACTGGCAAG
GAAGGTGTGATTTACAATGGCCTCAGTGACTGGCTGTATGAAGAGGAGATTTTGAAGACACACATCGCAC
ACTGGTGGTCTCCGGATGGCACGAGACTCGCCTACGCCGCCATCAATGATTCCCGTGTCCCCATCATGGA
GCTCCCAACTTACACCGGCTCCATCTACCCCACCGTGAAGCCCTACCACTATCCCAAGGCTGGAAGTGAG
AACCCCAGCATTTCCCTACACGTTATTGGCTTAAATGGACCCACCCATGATCTGGAGATGATGCCGCCTG
ATGATCCACGGATGAGGGAGTACTACATCACCATGGTGAAGTGGGCCACCAGCACCAAGGTCGCCGTGAC
CTGGCTGAACCGGGCGCAGAACGTGTCCATCCTCACCCTCTGCGACGCCACCACGGGGGTCTGCACGAAG
AAACACGAGGATGAAAGTGAGGCCTGGCTCCACAGACAGAATGAAGAACCTGTGTTCTCCAAGGATGGCC
GAAAGTTTTTCTTCATCAGAGCCATCCCCCAGGGAGGACGAGGGAAATTCTATCACATCACGGTGTCCTC
GTCCCAGCCCAACAGCAGCAACGACAACATCCAGTCCATCACCTCCGGGGACTGGGACGTGACCAAGATC
CTAGCCTACGATGAGAAGGGGAATAAGATCTACTTCCTGAGCACGGAGGACCTGCCTCGGAGACGACAAC
TCTACAGTGCCAACACGGTGGGCAACTTCAACAGGCAGTGCCTCTCCTGTGACCTGGTTGAGAACTGCAC
CTACTTCAGCGCTTCCTTCAGCCATAGCATGGACTTCTTCCTGCTCAAGTGCGAAGGTCCTGGTGTTCCT
ATGGTGACGGTGCACAACACAACAGATAAGAAAAAAATGTTTGACCTAGAAACAAATGAACATGTCAAGA
AGGCCATAAATGACCGACAGATGCCTAAAGTGGAATACAGGGACATTGAGATTGATGATTACAACCTGCC
CATGCAGATACTGAAGCCAGCAACCTTCACCGACACCACCCACTACCCTCTGCTCCTGGTGGTGGATGGC
ACCCCGGGCAGCCAGAGTGTGGCTGAGAAGTTCGAGGTGAGCTGGGAGACGGTGATGGTGAGCAGCCACG
GCGCGGTGGTGGTAAAGTGTGACGGCCGTGGCAGCGGCTTCCAAGGGACCAAGCTCCTGCACGAAGTGAG
GCGGCGGCTGGGCTTGCTGGAGGAGAAGGACCAGATGGAGGCCGTGCGGACGATGCTGAAGGAGCAGTAC
ATTGACAGGACGCGCGTGGCCGTGTTTGGGAAGGATTACGGTGGCTACCTGAGCACCTACATCCTCCCAG
CAAAGGGAGAAAATCAAGGCCAGACATTCACCTGCGGCTCTGCTCTCTCTCCAATAACAGACTTCAAACT
CTATGCCTCTGCGTTTTCCGAGAGGTACTTGGGCCTCCATGGACTTGACAACAGAGCATACGAGATGACC
AAGGTAGCCCATCGAGTCTCCGCGCTGGAAGAACAGCAGTTCCTGATCATTCATCCCACTGCCGATGAAA
AAATTCATTTCCAGCACACAGCAGAACTCATTACACAACTAATTAGGGGAAAGGCTAATTACAGCTTACA
GATTTACCCGGACGAAAGCCATTACTTTACCAGCTCCAGCCTCAAACAGCATCTGTACCGGTCCATCATC
AACTTCTTCGTGGAATGCTTCAGGATCCAGGACAAACTGCCGACAGTCACAGCGAAAGAGGACGAGGAGG
AGGAC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC214014 representing NM_130797
Red=Cloning site Green=Tags(s)

MASLYQRFTGKINTSRSFPAPPEASHLLGGQGPEEDGGAGAKPLGPRAQAAAPRERGGGGGGAGGRPRFQ
YQARSDGDEEDELVGSNPPQRNWKGIAIALLVILVICSLIVTSVILLTPAEDNSLSQKKKVTVEDLFSED
FKIHDPEAKWISDTEFIYREQKGTVRLWNVETNTSTVLIEGKKIESLRAIRYEISPDREYALFSYNVEPI
YQHSYTGYYVLSKIPHGDPQSLDPPEVSNAKLQYAGWGPKGQQLIFIFENNIYYCAHVGKQAIRVVSTGK
EGVIYNGLSDWLYEEEILKTHIAHWWSPDGTRLAYAAINDSRVPIMELPTYTGSIYPTVKPYHYPKAGSE
NPSISLHVIGLNGPTHDLEMMPPDDPRMREYYITMVKWATSTKVAVTWLNRAQNVSILTLCDATTGVCTK
KHEDESEAWLHRQNEEPVFSKDGRKFFFIRAIPQGGRGKFYHITVSSSQPNSSNDNIQSITSGDWDVTKI
LAYDEKGNKIYFLSTEDLPRRRQLYSANTVGNFNRQCLSCDLVENCTYFSASFSHSMDFFLLKCEGPGVP
MVTVHNTTDKKKMFDLETNEHVKKAINDRQMPKVEYRDIEIDDYNLPMQILKPATFTDTTHYPLLLVVDG
TPGSQSVAEKFEVSWETVMVSSHGAVVVKCDGRGSGFQGTKLLHEVRRRLGLLEEKDQMEAVRTMLKEQY
IDRTRVAVFGKDYGGYLSTYILPAKGENQGQTFTCGSALSPITDFKLYASAFSERYLGLHGLDNRAYEMT
KVAHRVSALEEQQFLIIHPTADEKIHFQHTAELITQLIRGKANYSLQIYPDESHYFTSSSLKQHLYRSII
NFFVECFRIQDKLPTVTAKEDEEED

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_130797
ORF Size 2595 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_130797.1, NP_570629.1
RefSeq Size 3712 bp
RefSeq ORF 2598 bp
Locus ID 1804
UniProt ID P42658
Cytogenetics 7q36.2
Domains DPPIV_N_term, Peptidase_S9
Protein Families Druggable Genome, Protease, Transmembrane
MW 97.4 kDa
Summary This gene encodes a single-pass type II membrane protein that is a member of the peptidase S9B family of serine proteases. This protein has no detectable protease activity, most likely due to the absence of the conserved serine residue normally present in the catalytic domain of serine proteases. However, it does bind specific voltage-gated potassium channels and alters their expression and biophysical properties. Variations in this gene may be associated with susceptibility to amyotrophic lateral sclerosis and with idiopathic ventricular fibrillation. Alternative splicing results in multiple transcript variants. provided by RefSeq, Mar 2014
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Documents
Resources
"NM_130797" in other vectors (6)
SKU Description Size Price
RC214014L1 Lenti-ORF clone of DPP6 (Myc-DDK-tagged)-Human dipeptidyl-peptidase 6 (DPP6), transcript variant 1 10 ug
$1,488.00
RC214014L2 Lenti-ORF clone of DPP6 (mGFP-tagged)-Human dipeptidyl-peptidase 6 (DPP6), transcript variant 1 10 ug
$1,488.00
RC214014L3 Lenti-ORF clone of DPP6 (Myc-DDK-tagged)-Human dipeptidyl-peptidase 6 (DPP6), transcript variant 1 10 ug
$1,488.00
RC214014L4 Lenti-ORF clone of DPP6 (mGFP-tagged)-Human dipeptidyl-peptidase 6 (DPP6), transcript variant 1 10 ug
$1,488.00
RG214014 DPP6 (tGFP-tagged) - Human dipeptidyl-peptidase 6 (DPP6), transcript variant 1 10 ug
$1,388.00
SC310075 DPP6 (untagged)-Human dipeptidyl-peptidase 6 (DPP6), transcript variant 1 10 ug
$1,268.00

Plasmid Map for RC214014

Download sequence for

Cloning scheme for RC214014

Circular map for RC214014

file-alt Citations

Use of whole exome sequencing for the identification of ito-based arrhythmia mechanism and therapy
Sturm, Kline, Glynn et al
J Am Heart Assoc (2015) 4 (5) DOI: 10.1161/JAHA.114.001762
Cited by 1
AI Summary: OriGene products, specifically the Human DPP6 isoform 1 (NM_130797) and Kv4.3 clones, were used as templates for cloning and functional studies that helped identify and characterize a novel DPP6 variant contributing to arrhythmia in this study.
Snippet: Molecular Biology. Human DPP6 isoform 1 (NM_130797) and K v 4.3 was acquired from Origene and used as a template to generate PCR fragments for cloning into pcDNA3.1+, pDsRed‐Monomer‐N1, and pcDNA6/myc‐HisB. ... Cited by 1 Cite SaveSaving
Showing 1-1 of 1 papers.
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