SERPINB10 (NM_005024) Human Tagged ORF Clone

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SKU
RC213932
SERPINB10 (Myc-DDK-tagged)-Human serpin peptidase inhibitor, clade B (ovalbumin), member 10 (SERPINB10)
  $457.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol SERPINB10
Synonyms PI-10; PI10
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC213932 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGACTCTCTAGCAACATCAATCAACCAGTTTGCCCTGGAGTTGAGCAAAAAGCTAGCTGAATCTGCTC
AGGGTAAAAATATCTTCTTTTCTTCCTGGAGCATCTCAACTTCCTTGACCATAGTGTATTTGGGCGCCAA
AGGTACCACTGCAGCCCAAATGGCCCAGGTGCTTCAATTTAACAGAGACCAGGGAGTCAAATGTGACCCT
GAAAGTGAAAAAAAAAGGAAAATGGAATTCAACTTGAGCAACTCGGAAGAAATACACTCTGATTTCCAAA
CACTTATCTCAGAAATCCTCAAGCCCAACGATGACTACTTACTTAAAACAGCCAATGCGATATATGGAGA
GAAAACGTATGCATTTCACAATAAATATTTAGAAGACATGAAAACATATTTTGGTGCAGAACCTCAGCCT
GTTAACTTTGTGGAAGCTTCTGATCAAATCAGAAAGGACATCAACTCTTGGGTTGAAAGACAGACCGAGG
GTAAAATCCAGAATCTCCTGCCTGATGACTCTGTGGATTCCACAACCAGGATGATTCTGGTGAACGCCCT
ATACTTTAAAGGAATCTGGGAACATCAATTCTTAGTGCAAAACACCACAGAAAAGCCTTTTAGAATAAAC
GAGACTACAAGCAAACCAGTGCAAATGATGTTTATGAAGAAAAAGCTTCACATTTTTCACATAGAAAAGC
CAAAAGCAGTGGGCCTTCAACTCTACTACAAAAGCCGTGACCTCAGCCTGCTTATACTACTGCCAGAAGA
CATTAATGGGCTGGAACAGCTGGAAAAGGCCATCACCTATGAGAAGCTGAATGAGTGGACCAGTGCAGAC
ATGATGGAGTTGTATGAAGTGCAGCTACACCTTCCCAAGTTCAAGCTGGAAGACAGTTATGATCTCAAGT
CAACCCTGAGCAGTATGGGGATGAGTGATGCCTTCAGCCAAAGCAAAGCTGATTTCTCAGGAATGTCTTC
AGCAAGAAACCTATTTTTGTCCAATGTTTTCCATAAGGCTTTTGTGGAAATAAATGAACAAGGTACTGAA
GCTGCAGCTGGCAGTGGGAGTGAGATAGATATACGAATTAGAGTCCCATCCATTGAATTCAATGCAAATC
ACCCATTCCTCTTCTTCATCAGGCACAATAAAACCAACACCATTCTTTTTTATGGAAGATTATGCTCCCC
C


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC213932 protein sequence
Red=Cloning site Green=Tags(s)

MDSLATSINQFALELSKKLAESAQGKNIFFSSWSISTSLTIVYLGAKGTTAAQMAQVLQFNRDQGVKCDP
ESEKKRKMEFNLSNSEEIHSDFQTLISEILKPNDDYLLKTANAIYGEKTYAFHNKYLEDMKTYFGAEPQP
VNFVEASDQIRKDINSWVERQTEGKIQNLLPDDSVDSTTRMILVNALYFKGIWEHQFLVQNTTEKPFRIN
ETTSKPVQMMFMKKKLHIFHIEKPKAVGLQLYYKSRDLSLLILLPEDINGLEQLEKAITYEKLNEWTSAD
MMELYEVQLHLPKFKLEDSYDLKSTLSSMGMSDAFSQSKADFSGMSSARNLFLSNVFHKAFVEINEQGTE
AAAGSGSEIDIRIRVPSIEFNANHPFLFFIRHNKTNTILFYGRLCSP

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene |
ACCN NM_005024
ORF Size 1191 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_005024.3
RefSeq Size 1194 bp
RefSeq ORF 1194 bp
Locus ID 5273
UniProt ID P48595, B2RC45
Cytogenetics 18q22.1
Domains SERPIN
Protein Families Druggable Genome
MW 45.4 kDa
Summary This gene is a member of the serpin peptidase inhibitor, clade B family and is found in a cluster of other similar genes on chromosome 18. The protein encoded by this gene appears to help control the regulation of protease functions during hematopoiesis. Variations in this gene may increase the risk of prostate cancer. provided by RefSeq, Dec 2015
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Documents
Resources
"NM_005024" in other vectors (6)
SKU Description Size Price
RC213932L1 Lenti ORF clone of Human serpin peptidase inhibitor, clade B (ovalbumin), member 10 (SERPINB10), Myc-DDK-tagged 10 ug
$757.00
RC213932L2 Lenti ORF clone of Human serpin peptidase inhibitor, clade B (ovalbumin), member 10 (SERPINB10), mGFP tagged 10 ug
$757.00
RC213932L3 Lenti ORF clone of Human serpin peptidase inhibitor, clade B (ovalbumin), member 10 (SERPINB10), Myc-DDK-tagged 10 ug
$757.00
RC213932L4 Lenti ORF clone of Human serpin peptidase inhibitor, clade B (ovalbumin), member 10 (SERPINB10), mGFP tagged 10 ug
$757.00
RG213932 SERPINB10 (tGFP-tagged) - Human serpin peptidase inhibitor, clade B (ovalbumin), member 10 (SERPINB10) 10 ug
$489.00 $657.00
SC117017 SERPINB10 (untagged)-Human serpin peptidase inhibitor, clade B (ovalbumin), member 10 (SERPINB10) 10 ug
$457.00

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