PHF21A (NM_001101802) Human Tagged ORF Clone
$934.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | PHF21A |
| Synonyms | BHC80; BM-006; IDDBCS; NEDMS |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_001101802 |
| ORF Size | 2040 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_001101802.3 |
| RefSeq Size | 7332 bp |
| RefSeq ORF | 2043 bp |
| Locus ID | 51317 |
| UniProt ID | Q96BD5 |
| Cytogenetics | 11p11.2 |
| Protein Families | Druggable Genome, Transcription Factors |
| MW | 74.7 kDa |
| Summary | The PHF21A gene encodes BHC80, a component of a BRAF35 (MIM 605535)/histone deacetylase (HDAC; see MIM 601241) complex (BHC) that mediates repression of neuron-specific genes through the cis-regulatory element known as repressor element-1 (RE1) or neural restrictive silencer (NRS) (Hakimi et al., 2002 PubMed 12032298).supplied by OMIM, Nov 2010 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_001101802" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC213929L1 | Lenti ORF clone of Human PHD finger protein 21A (PHF21A), transcript variant 1, Myc-DDK-tagged | 10 ug |
$1,234.00
|
|
| RC213929L2 | Lenti ORF clone of Human PHD finger protein 21A (PHF21A), transcript variant 1, mGFP tagged | 10 ug |
$1,234.00
|
|
| RC213929L3 | Lenti ORF clone of Human PHD finger protein 21A (PHF21A), transcript variant 1, Myc-DDK-tagged | 10 ug |
$1,234.00
|
|
| RC213929L4 | Lenti ORF clone of Human PHD finger protein 21A (PHF21A), transcript variant 1, mGFP tagged | 10 ug |
$1,234.00
|
|
| RG213929 | PHF21A (tGFP-tagged) - Human PHD finger protein 21A (PHF21A), transcript variant 1 | 10 ug |
$1,134.00
|
|
| SC316961 | PHF21A (untagged)-Human PHD finger protein 21A (PHF21A), transcript variant 1 | 10 ug |
$686.00
|
|
Citations
A type 2 diabetes-associated SNP in KCNQ1 (rs163184) modulates the binding activity of the locus for Sp3 and Lsd1/Kdm1a, potentially affecting CDKN1C expression
Hiramoto, Udagawa, Ishibashi et al
Int J Mol Med (2018) 41 (2), 717-728 DOI: 10.3892/ijmm.2017.3273
Hiramoto, Udagawa, Ishibashi et al
Int J Mol Med (2018) 41 (2), 717-728 DOI: 10.3892/ijmm.2017.3273
Cited by 16
AI Summary: In this study, OriGene products—specifically the human SP3 shRNA plasmid (TL313206) and human KDM1A (LSD1) shRNA plasmid (TL313865)—were used to knock down SP3 and KDM1A gene expression, enabling the authors to investigate how these proteins interact with the KCNQ1 SNP rs163184 locus and affect CDKN1C gene expression.
A type 2 diabetes-associated SNP in KCNQ1 (rs163184) modulates the binding activity of the locus for Sp3 and Lsd1/Kdm1a, potentially affecting CDKN1C expression
HIRAMOTO, UDAGAWA, ISHIBASHI et al
International Journal of Molecular Medicine (2017)
HIRAMOTO, UDAGAWA, ISHIBASHI et al
International Journal of Molecular Medicine (2017)
AI Summary: OriGene products contributed to this study by providing the pCMV6-Entry vector and the cDNA clone (catalog no. RC213929), which were used for gene expression and functional analyses.
Snippet: The cDNA were inserted into a pCMV6-Entry vector (OriGene Technologies, Inc., Rockville, MD, USA) and sequenced with an ABI 3700 sequence analyzer (Applied Biosystems;Thermo Fisher Scientific, Inc.) no. RC213929) were obtained from OriGene Technologies
Showing 1-2 of 2 papers.
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