TRIM14 (NM_033220) Human Tagged ORF Clone

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SKU
RC213915
TRIM14 (Myc-DDK-tagged)-Human tripartite motif containing 14 (TRIM14), transcript variant 3
  $457.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol TRIM14
Synonyms KIAA0129
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC213915 representing NM_033220
Red=Cloning site Blue=ORF Green=Tags(s)

CTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCCGGCGC
GCC
C

ATGGCGGGCGCGGCGACCGGGAGCCGGACCCCTGGGAGGTCGGAGCTTGTCGAGGGATGCGGCTGGCGCT
GCCCGGAGCATGGCGACCGCGTGGCTGAGCTCTTCTGTCGCCGCTGCCGCCGCTGCGTGTGCGCGCTTTG
CCCGGTGCTGGGCGCGCACCGTGGCCACCCTGTGGGCCTGGCGCTGGAGGCAGCGGTGCACGTGCAGAAA
CTCAGCCAAGAATGTTTAAAGCAGCTGGCAATCAAGAAGCAGCAGCACATTGACAACATAACCCAGATAG
AAGATGCCACCGAGAAGCTCAAGGCTAATGCAGAGTCAAGTAAAACCTGGCTGAAGGGGAAATTCACTGA
ACTCAGATTACTACTTGACGAAGAGGAAGCGCTGGCCAAGAAATTCATTGATAAAAACACGCAGCTTACC
CTCCAGGTGTACAGGGAACAAGCTGACTCTTGCAGAGAGCAACTTGACATCATGAATGATCTCTCCAACA
GGGTCTGGAGTATCAGCCAGGAGCCCGATCCTGTCCAGAGGCTTCAGGCATACACGGCCACCGAGCAGGA
GATGCAGCAGCAGATGAGCCTCGGGGAGCTGTGCCATCCCGTGCCCCTCTCCTTTGAGCCCGTCAAGAGC
TTCTTTAAGGGCCTCGTGGAAGCCGTGGAGAGTACATTACAGACGCCATTGGACATTCGCCTTAAGGAAA
GCATAAACTGCCAGCTCTCAGACCCTTCCAGCACCAAGCCAGGTACCTTGTTGAAAACCAGCCCCTCACC
AGAGCGATCGCTATTGCTGAAATACGCGCGCACGCCCACGCTGGATCCTGACACGATGCACGCGCGCCTG
CGCCTGTCCGCCGATCGCCTGACGGTGCGCTGCGGCCTGCTGGGCAGCCTGGGGCCCGTGCCCGTGCTGC
GGTTCGACGCGCTCTGGCAAGTGCTGGCTCGTGACTGCTTCGCCACCGGCCGCCACTACTGGGAGGTTGA
CGTGCAGGAGGCGGGCGCCGGCTGGTGGGTGGGCGCGGCCTACGCCTCCCTTCGGCGCCGCGGGGCCTCG
GCCGCCGCCCGCCTGGGCTGCAACCGCCAGTCCTGGTGCCTCAAGCGCTACGACCTTGAGTACTGGGCCT
TCCACGACGGCCAGCGCAGCCGCCTGCGGCCCCGCGACGACCTCGACCGGCTCGGCGTCTTCCTGGACTA
CGAGGCCGGCGTCCTCGCCTTCTACGACGTGACGGGCGGCATGAGCCACCTGCATACCTTCCGCGCCACG
TTCCAGGAGCCGCTCTACCCGGCCCTGCGGCTCTGGGAGGGGGCCATCAGCATCCCCCGGCTGCCC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC213915 representing NM_033220
Red=Cloning site Green=Tags(s)

MAGAATGSRTPGRSELVEGCGWRCPEHGDRVAELFCRRCRRCVCALCPVLGAHRGHPVGLALEAAVHVQK
LSQECLKQLAIKKQQHIDNITQIEDATEKLKANAESSKTWLKGKFTELRLLLDEEEALAKKFIDKNTQLT
LQVYREQADSCREQLDIMNDLSNRVWSISQEPDPVQRLQAYTATEQEMQQQMSLGELCHPVPLSFEPVKS
FFKGLVEAVESTLQTPLDIRLKESINCQLSDPSSTKPGTLLKTSPSPERSLLLKYARTPTLDPDTMHARL
RLSADRLTVRCGLLGSLGPVPVLRFDALWQVLARDCFATGRHYWEVDVQEAGAGWWVGAAYASLRRRGAS
AAARLGCNRQSWCLKRYDLEYWAFHDGQRSRLRPRDDLDRLGVFLDYEAGVLAFYDVTGGMSHLHTFRAT
FQEPLYPALRLWEGAISIPRLP

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites AscI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_033220
ORF Size 1326 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_033220.1, NP_150089.1
RefSeq Size 1789 bp
RefSeq ORF 1328 bp
Locus ID 9830
Cytogenetics 9q22.33
Domains PRY, SPRY, zf-B_box
Protein Families Druggable Genome
MW 49.6 kDa
Summary The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to cytoplasmic bodies and its function has not been determined. Alternative splicing results in multiple transcript variants. provided by RefSeq, Mar 2010
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Documents
Resources
"NM_033220" in other vectors (4)
SKU Description Size Price
RC213915L3 Lenti ORF clone of Human tripartite motif containing 14 (TRIM14), transcript variant 3, Myc-DDK-tagged 10 ug
$757.00
RC213915L4 Lenti ORF clone of Human tripartite motif containing 14 (TRIM14), transcript variant 3, mGFP tagged 10 ug
$757.00
RG213915 TRIM14 (tGFP-tagged) - Human tripartite motif containing 14 (TRIM14), transcript variant 3 10 ug
$657.00
SC110097 TRIM14 (untagged)-Human tripartite motif containing 14 (TRIM14), transcript variant 3 10 ug
$457.00

Plasmid Map for RC213915

Download sequence for

Cloning scheme for RC213915

Circular map for RC213915

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