MEK7 (MAP2K7) (NM_145185) Human Tagged ORF Clone
$457.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | MEK7 |
| Synonyms | JNKK2; MAPKK7; MEK; MEK 7; MKK7; PRKMK7; SAPKK-4; SAPKK4 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_145185 |
| ORF Size | 1257 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_145185.4 |
| RefSeq Size | 3386 bp |
| RefSeq ORF | 1260 bp |
| Locus ID | 5609 |
| UniProt ID | O14733 |
| Cytogenetics | 19p13.2 |
| Protein Families | Druggable Genome, Protein Kinase |
| Protein Pathways | ErbB signaling pathway, Fc epsilon RI signaling pathway, GnRH signaling pathway, MAPK signaling pathway, Neurotrophin signaling pathway, T cell receptor signaling pathway, Toll-like receptor signaling pathway |
| MW | 47.3 kDa |
| Summary | The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase specifically activates MAPK8/JNK1 and MAPK9/JNK2, and this kinase itself is phosphorylated and activated by MAP kinase kinase kinases including MAP3K1/MEKK1, MAP3K2/MEKK2,MAP3K3/MEKK5, and MAP4K2/GCK. This kinase is involved in the signal transduction mediating the cell responses to proinflammatory cytokines, and environmental stresses. Alternative splicing results in multiple transcript variants. provided by RefSeq, Jul 2014 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_145185" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC213868L1 | Lenti ORF clone of Human mitogen-activated protein kinase kinase 7 (MAP2K7), Myc-DDK-tagged | 10 ug |
$757.00
|
|
| RC213868L2 | Lenti ORF clone of Human mitogen-activated protein kinase kinase 7 (MAP2K7), mGFP tagged | 10 ug |
$757.00
|
|
| RC213868L3 | Lenti ORF clone of Human mitogen-activated protein kinase kinase 7 (MAP2K7), Myc-DDK-tagged | 10 ug |
$757.00
|
|
| RC213868L4 | Lenti ORF clone of Human mitogen-activated protein kinase kinase 7 (MAP2K7), mGFP tagged | 10 ug |
$757.00
|
|
| RG213868 | MAP2K7 (tGFP-tagged) - Human mitogen-activated protein kinase kinase 7 (MAP2K7) | 10 ug |
$489.00
$657.00
|
|
| SC309544 | MAP2K7 (untagged)-Human mitogen-activated protein kinase kinase 7 (MAP2K7) | 10 ug |
$457.00
|
|
Citations
Truncation- and motif-based pan-cancer analysis reveals tumor-suppressing kinases
Hudson, Stephenson, Li et al
Sci Signal (2018) 11 (526) DOI: 10.1126/scisignal.aan6776
Hudson, Stephenson, Li et al
Sci Signal (2018) 11 (526) DOI: 10.1126/scisignal.aan6776
AI Summary: OriGene contributed pCMV6-Entry vectors containing MAP2K4 and MAP2K7, which were used for functional studies of tumor-suppressing kinases in this pan-cancer analysis.
Snippet: BRAF and MAP3K13 complementary DNA were prepared from RNA extracted from human embryonic kidney (HEK) 293T cells. PRKCQ was bought in the pENTR vector (Ultimate Human ORF Library, Life Technologies), MAP2K4 and MAP2K7 were bought in pCMV6-Entry vectors (Origene), and MLK4 was purchased in pReceiver-M12 FLAG vector (GeneCopoeia)
Truncation and Motif Based Pan-Cancer Analysis Highlights Novel Tumor Suppressing Kinases
Hudson, Stepehnson, Li et al
bioRxiv (2018)
Hudson, Stepehnson, Li et al
bioRxiv (2018)
Snippet: BRAF and MAP3K13 cDNA w ere prepared from RNA extracted from HEK293T cells. PRKCQ was bought in the pENTR vector (Ultimate Human ORF Library -‐ Life Technologies), MAP2K4 and MAP2K7 were bought in pCMV6 -‐ Entry vectors (Origene), and MLK4 was purchased in pReceiver -‐ M12 FLAG ...
A road map to evaluate the proteome-wide selectivity of covalent kinase inhibitors
Lanning, Whitby, Dix et al
Nat. Chem. Biol. (2014)
Lanning, Whitby, Dix et al
Nat. Chem. Biol. (2014)
Cited by 369
Showing 1-3 of 3 papers.
Powered by BizGenius
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
Related Products
- Price:
- Actual Price:
Welcome to OriGene AI!
I'm your AI-powered guide to everything OriGene.
Ask me anything:
- Product specs
- Ordering help
- Technical questions
- How to find exactly what your experiment needs
Prefer a live agent? Connect with us by clicking the green chat icon, as seen in the image below or email our team at custsupport@origene.com.
