TARBP1 (NM_005646) Human Tagged Lenti ORF Clone

  • LentiORF®
SKU
RC213800L3
Lenti-ORF clone of TARBP1 (Myc-DDK-tagged)-Human TAR (HIV-1) RNA binding protein 1 (TARBP1)
  $1,686.00
3 Weeks*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol TARBP1
Synonyms TRM3; TRMT3; TRP-185; TRP185
Vector pLenti-C-Myc-DDK-P2A-Puro
E. coli Selection Chloramphenicol (34 ug/mL)
Mammalian Cell Selection Puromycin
Sequence Data
ORF Nucleotide Sequence
The ORF insert of this clone is exactly the same as(RC213800).
Restriction Sites SgfI-MluI Cloning Scheme for this gene |
ACCN NM_005646
ORF Size 4863 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_005646.2, NP_005637.2
RefSeq Size 5130 bp
RefSeq ORF 4866 bp
Locus ID 6894
UniProt ID Q13395
Cytogenetics 1q42.2
Domains SpoU_methylase
MW 181.5 kDa
Summary HIV-1, the causative agent of acquired immunodeficiency syndrome (AIDS), contains an RNA genome that produces a chromosomally integrated DNA during the replicative cycle. Activation of HIV-1 gene expression by the transactivator Tat is dependent on an RNA regulatory element (TAR) located downstream of the transcription initiation site. This element forms a stable stem-loop structure and can be bound by either the protein encoded by this gene or by RNA polymerase II. This protein may act to disengage RNA polymerase II from TAR during transcriptional elongation. Alternatively spliced transcripts of this gene may exist, but their full-length natures have not been determined. provided by RefSeq, Jul 2008
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Documents
Resources
"NM_005646" in other vectors (4)
SKU Description Size Price
RC213800 TARBP1 (Myc-DDK-tagged)-Human TAR (HIV-1) RNA binding protein 1 (TARBP1) 10 ug
$589.00 $1,386.00
RC213800L4 Lenti-ORF clone of TARBP1 (mGFP-tagged)-Human TAR (HIV-1) RNA binding protein 1 (TARBP1) 10 ug
$1,686.00
RG213800 TARBP1 (tGFP-tagged) - Human TAR (HIV-1) RNA binding protein 1 (TARBP1) 10 ug
$1,586.00
SC309720 TARBP1 (untagged)-Human TAR (HIV-1) RNA binding protein 1 (TARBP1) 10 ug
$1,526.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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