TARBP1 (NM_005646) Human Tagged ORF Clone
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | TARBP1 |
| Synonyms | TRM3; TRMT3; TRP-185; TRP185 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
![]() Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_005646 |
| ORF Size | 4863 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_005646.2, NP_005637.2 |
| RefSeq Size | 5130 bp |
| RefSeq ORF | 4866 bp |
| Locus ID | 6894 |
| UniProt ID | Q13395 |
| Cytogenetics | 1q42.2 |
| Domains | SpoU_methylase |
| MW | 181.5 kDa |
| Summary | HIV-1, the causative agent of acquired immunodeficiency syndrome (AIDS), contains an RNA genome that produces a chromosomally integrated DNA during the replicative cycle. Activation of HIV-1 gene expression by the transactivator Tat is dependent on an RNA regulatory element (TAR) located downstream of the transcription initiation site. This element forms a stable stem-loop structure and can be bound by either the protein encoded by this gene or by RNA polymerase II. This protein may act to disengage RNA polymerase II from TAR during transcriptional elongation. Alternatively spliced transcripts of this gene may exist, but their full-length natures have not been determined. provided by RefSeq, Jul 2008 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_005646" in other vectors (4)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC213800L3 | Lenti-ORF clone of TARBP1 (Myc-DDK-tagged)-Human TAR (HIV-1) RNA binding protein 1 (TARBP1) | 10 ug |
$1,686.00
|
|
| RC213800L4 | Lenti-ORF clone of TARBP1 (mGFP-tagged)-Human TAR (HIV-1) RNA binding protein 1 (TARBP1) | 10 ug |
$1,686.00
|
|
| RG213800 | TARBP1 (tGFP-tagged) - Human TAR (HIV-1) RNA binding protein 1 (TARBP1) | 10 ug |
$1,586.00
|
|
| SC309720 | TARBP1 (untagged)-Human TAR (HIV-1) RNA binding protein 1 (TARBP1) | 10 ug |
$1,526.00
|
|
Citations
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