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MTTP (NM_000253) Human Tagged ORF Clone

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SKU
RC213699
MTTP (Myc-DDK-tagged)-Human microsomal triglyceride transfer protein (MTTP)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
  $819.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol MTTP
Synonyms ABL; MTP
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC213699 representing NM_000253
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGATTCTTCTTGCTGTGCTTTTTCTCTGCTTCATTTCCTCATATTCAGCTTCTGTTAAAGGTCACACAA
CTGGTCTCTCATTAAATAATGACCGGCTGTACAAGCTCACGTACTCCACTGAAGTTCTTCTTGATCGGGG
CAAAGGAAAACTGCAAGACAGCGTGGGCTACCGCATTTCCTCCAACGTGGATGTGGCCTTACTATGGAGG
AATCCTGATGGTGATGATGACCAGTTGATCCAAATAACGATGAAGGATGTAAATGTTGAAAATGTGAATC
AGCAGAGAGGAGAGAAGAGCATCTTCAAAGGAAAAAGCCCATCTAAAATAATGGGAAAGGAAAACTTGGA
AGCTCTGCAAAGACCTACGCTCCTTCATCTAATCCATGGAAAGGTCAAAGAGTTCTACTCATATCAAAAT
GAGGCAGTGGCCATAGAAAATATCAAGAGAGGTCTGGCTAGCCTATTTCAGACACAGTTAAGCTCTGGAA
CCACCAATGAGGTAGATATCTCTGGAAATTGTAAAGTGACCTACCAGGCTCATCAAGACAAAGTGATCAA
AATTAAGGCCTTGGATTCATGCAAAATAGCGAGGTCTGGATTTACGACCCCAAATCAGGTCTTGGGTGTC
AGTTCAAAAGCTACATCTGTCACCACCTATAAGATAGAAGACAGCTTTGTTATAGCTGTGCTTGCTGAAG
AAACACACAATTTTGGACTGAATTTCCTACAAACCATTAAGGGGAAAATAGTATCGAAGCAGAAATTAGA
GCTGAAGACAACCGAAGCAGGCCCAAGATTGATGTCTGGAAAGCAGGCTGCAGCCATAATCAAAGCAGTT
GATTCAAAGTACACGGCCATTCCCATTGTGGGGCAGGTCTTCCAGAGCCACTGTAAAGGATGTCCTTCTC
TCTCGGAGCTCTGGCGGTCCACCAGGAAATACCTGCAGCCTGACAACCTTTCCAAGGCTGAGGCTGTCAG
AAACTTCCTGGCCTTCATTCAGCACCTCAGGACTGCGAAGAAAGAAGAGATCCTTCAAATACTAAAGATG
GAAAATAAGGAAGTATTACCTCAGCTGGTGGATGCTGTCACCTCTGCTCAGACCTCAGACTCATTAGAAG
CCATTTTGGACTTTTTGGATTTCAAAAGTGACAGCAGCATTATCCTCCAGGAGAGGTTTCTCTATGCCTG
TGGATTTGCTTCTCATCCCAATGAAGAACTCCTGAGAGCCCTCATTAGTAAGTTCAAAGGTTCTATTGGT
AGCAGTGACATCAGAGAAACTGTTATGATCATCACTGGGACACTTGTCAGAAAGTTGTGTCAGAATGAAG
GCTGCAAACTCAAAGCAGTAGTGGAAGCTAAGAAGTTAATCCTGGGAGGACTTGAAAAAGCAGAGAAAAA
AGAGGACACCAGGATGTATCTGCTGGCTTTGAAGAATGCCCTGCTTCCAGAAGGCATCCCAAGTCTTCTG
AAGTATGCAGAAGCAGGAGAAGGGCCCATCAGCCACCTGGCTACCACTGCTCTCCAGAGATATGATCTCC
CTTTCATAACTGATGAGGTGAAGAAGACCTTAAACAGAATATACCACCAAAACCGTAAAGTTCATGAAAA
GACTGTGCGCACTGCTGCAGCTGCTATCATTTTAAATAACAATCCATCCTACATGGACGTCAAGAACATC
CTGCTGTCTATTGGGGAGCTTCCCCAAGAAATGAATAAATACATGCTCGCCATTGTTCAAGACATCCTAC
GTTTTGAAATGCCTGCAAGCAAAATTGTCCGTCGAGTTCTGAAGGAAATGGTCGCTCACAATTATGACCG
TTTCTCCAGGAGTGGATCTTCTTCTGCCTACACTGGCTACATAGAACGTAGTCCCCGTTCGGCATCTACT
TACAGCCTAGACATTCTCTACTCGGGTTCTGGCATTCTAAGGAGAAGTAACCTGAACATCTTTCAGTACA
TTGGGAAGGCTGGTCTTCACGGTAGCCAGGTGGTTATTGAAGCCCAAGGACTGGAAGCCTTAATCGCAGC
CACCCCTGACGAGGGGGAGGAGAACCTTGACTCCTATGCTGGTATGTCAGCCATCCTCTTTGATGTTCAG
CTCAGACCTGTCACCTTTTTCAACGGATACAGTGATTTGATGTCCAAAATGCTGTCAGCATCTGGCGACC
CTATCAGTGTGGTGAAAGGACTTATTCTGCTAATAGATCATTCTCAGGAACTTCAGTTACAATCTGGACT
AAAAGCCAATATAGAGGTCCAGGGTGGTCTAGCTATTGATATTTCAGGTGCAATGGAGTTTAGCTTGTGG
TATCGTGAGTCTAAAACCCGAGTGAAAAATAGGGTGACTGTGGTAATAACCACTGACATCACAGTGGACT
CCTCTTTTGTGAAAGCTGGCCTGGAAACCAGTACAGAAACAGAAGCAGGCTTGGAGTTTATCTCCACAGT
GCAGTTTTCTCAGTACCCATTCTTAGTTTGCATGCAGATGGACAAGGATGAAGCTCCATTCAGGCAATTT
GAGAAAAAGTACGAAAGGCTGTCCACAGGCAGAGGTTATGTCTCTCAGAAAAGAAAAGAAAGCGTATTAG
CAGGATGTGAATTCCCGCTCCATCAAGAGAACTCAGAGATGTGCAAAGTGGTGTTTGCCCCTCAGCCGGA
TAGTACTTCCAGCGGATGGTTT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC213699 representing NM_000253
Red=Cloning site Green=Tags(s)

MILLAVLFLCFISSYSASVKGHTTGLSLNNDRLYKLTYSTEVLLDRGKGKLQDSVGYRISSNVDVALLWR
NPDGDDDQLIQITMKDVNVENVNQQRGEKSIFKGKSPSKIMGKENLEALQRPTLLHLIHGKVKEFYSYQN
EAVAIENIKRGLASLFQTQLSSGTTNEVDISGNCKVTYQAHQDKVIKIKALDSCKIARSGFTTPNQVLGV
SSKATSVTTYKIEDSFVIAVLAEETHNFGLNFLQTIKGKIVSKQKLELKTTEAGPRLMSGKQAAAIIKAV
DSKYTAIPIVGQVFQSHCKGCPSLSELWRSTRKYLQPDNLSKAEAVRNFLAFIQHLRTAKKEEILQILKM
ENKEVLPQLVDAVTSAQTSDSLEAILDFLDFKSDSSIILQERFLYACGFASHPNEELLRALISKFKGSIG
SSDIRETVMIITGTLVRKLCQNEGCKLKAVVEAKKLILGGLEKAEKKEDTRMYLLALKNALLPEGIPSLL
KYAEAGEGPISHLATTALQRYDLPFITDEVKKTLNRIYHQNRKVHEKTVRTAAAAIILNNNPSYMDVKNI
LLSIGELPQEMNKYMLAIVQDILRFEMPASKIVRRVLKEMVAHNYDRFSRSGSSSAYTGYIERSPRSAST
YSLDILYSGSGILRRSNLNIFQYIGKAGLHGSQVVIEAQGLEALIAATPDEGEENLDSYAGMSAILFDVQ
LRPVTFFNGYSDLMSKMLSASGDPISVVKGLILLIDHSQELQLQSGLKANIEVQGGLAIDISGAMEFSLW
YRESKTRVKNRVTVVITTDITVDSSFVKAGLETSTETEAGLEFISTVQFSQYPFLVCMQMDKDEAPFRQF
EKKYERLSTGRGYVSQKRKESVLAGCEFPLHQENSEMCKVVFAPQPDSTSSGWF

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_000253
ORF Size 2682 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_000253.4
RefSeq Size 3392 bp
RefSeq ORF 2685 bp
Locus ID 4547
UniProt ID P55157
Cytogenetics 4q23
Domains LPD_N
Protein Families Druggable Genome
MW 99.35 kDa
Summary MTP encodes the large subunit of the heterodimeric microsomal triglyceride transfer protein. Protein disulfide isomerase (PDI) completes the heterodimeric microsomal triglyceride transfer protein, which has been shown to play a central role in lipoprotein assembly. Mutations in MTP can cause abetalipoproteinemia. provided by RefSeq, Jul 2008
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"NM_000253" in other vectors (6)
SKU Description Size Price
RC213699L1 Lenti ORF clone of Human microsomal triglyceride transfer protein (MTTP), Myc-DDK-tagged 10 ug
$1,119.00
RC213699L2 Lenti ORF clone of Human microsomal triglyceride transfer protein (MTTP), mGFP tagged 10 ug
$1,119.00
RC213699L3 Lenti ORF clone of Human microsomal triglyceride transfer protein (MTTP), Myc-DDK-tagged 10 ug
$1,119.00
RC213699L4 Lenti ORF clone of Human microsomal triglyceride transfer protein (MTTP), mGFP tagged 10 ug
$1,119.00
RG213699 MTTP (tGFP-tagged) - Human microsomal triglyceride transfer protein (MTTP) 10 ug
$789.00 $1,019.00
SC119999 MTTP (untagged)-Human microsomal triglyceride transfer protein (MTTP) 10 ug
$819.00

Plasmid Map for RC213699

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC213699
Circular map for RC213699

file-alt Citations

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