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GPR22 (NM_005295) Human Tagged ORF Clone

  • TrueORF®
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SKU
RC213524
GPR22 (Myc-DDK-tagged)-Human G protein-coupled receptor 22 (GPR22)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $457.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol GPR22
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC213524 representing NM_005295
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTGTTTTTCTCCCATTCTGGAAATCAACATGCAGTCTGAATCTAACATTACAGTGCGAGATGACATTG
ATGACATCAACACCAATATGTACCAACCACTATCATATCCGTTAAGCTTTCAAGTGTCTCTCACCGGATT
TCTTATGTTAGAAATTGTGTTGGGACTTGGCAGCAACCTCACTGTATTGGTACTTTACTGCATGAAATCC
AACTTAATCAACTCTGTCAGTAACATTATTACAATGAATCTTCATGTACTTGATGTAATAATTTGTGTGG
GATGTATTCCTCTAACTATAGTTATCCTTCTGCTTTCACTGGAGAGTAACACTGCTCTCATTTGCTGTTT
CCATGAGGCTTGTGTATCTTTTGCAAGTGTCTCAACAGCAATCAACGTTTTTGCTATCACTTTGGACAGA
TATGACATCTCTGTAAAACCTGCAAACCGAATTCTGACAATGGGCAGAGCTGTAGTGTTAATGATATCCA
TTTGGATTTTTTCTTTTTTCTCTTTCCTGATTCCTTTTATTGAGGTAAATTTTTTCAGTCTTCAAAGTGG
AAATACCTGGGAAAACAAGACACTTTTATGTGTCAGTACAAATGAATACTACACTGAACTGGGAATGTAT
TATCACCTGTTAGTACAGATCCCAATATTCTTTTTCACTGTTGTAGTAATGTTAATCACATACACCAAAA
TACTTCAGGCTCTTAATATTCGAATAGGCACAAGATTTTCAACAGGGCAGAAGAAGAAAGCAAGAAAGAA
AAAGACAATTTCTCTAACCACACAACATGAGGCTACAGACATGTCACAAAGCAGTGGTGGGAGAAATGTA
GTCTTTGGTGTAAGAACTTCAGTTTCTGTAATAATTGCCCTCCGGCGAGCTGTGAAACGACACCGTGAAC
GACGAGAAAGACAAAAGAGAGTCTTCAGGATGTCTTTATTGATTATTTCTACATTTCTTCTCTGCTGGAC
ACCAATTTCTGTTTTAAATACCACCATTTTATGTTTAGGCCCAAGTGACCTTTTAGTAAAATTAAGATTG
TGTTTTTTAGTCATGGCTTATGGAACAACTATATTTCACCCTCTATTATATGCATTCACTAGACAAAAAT
TTCAAAAGGTCTTGAAAAGTAAAATGAAAAAGCGAGTTGTTTCTATAGTAGAAGCTGATCCCCTGCCTAA
TAATGCTGTAATACACAACTCTTGGATAGATCCTAAAAGAAACAAAAAAATTACCTTTGAAGATAGTGAA
ATAAGAGAAAAATGTTTAGTGCCTCAGGTTGTCACAGAC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC213524 representing NM_005295
Red=Cloning site Green=Tags(s)

MCFSPILEINMQSESNITVRDDIDDINTNMYQPLSYPLSFQVSLTGFLMLEIVLGLGSNLTVLVLYCMKS
NLINSVSNIITMNLHVLDVIICVGCIPLTIVILLLSLESNTALICCFHEACVSFASVSTAINVFAITLDR
YDISVKPANRILTMGRAVVLMISIWIFSFFSFLIPFIEVNFFSLQSGNTWENKTLLCVSTNEYYTELGMY
YHLLVQIPIFFFTVVVMLITYTKILQALNIRIGTRFSTGQKKKARKKKTISLTTQHEATDMSQSSGGRNV
VFGVRTSVSVIIALRRAVKRHRERRERQKRVFRMSLLIISTFLLCWTPISVLNTTILCLGPSDLLVKLRL
CFLVMAYGTTIFHPLLYAFTRQKFQKVLKSKMKKRVVSIVEADPLPNNAVIHNSWIDPKRNKKITFEDSE
IREKCLVPQVVTD

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_005295
ORF Size 1299 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_005295.3
RefSeq Size 1302 bp
RefSeq ORF 1302 bp
Locus ID 2845
UniProt ID Q99680
Cytogenetics 7q22.3
Protein Families Druggable Genome, GPCR, Transmembrane
MW 49.1 kDa
Summary This gene is a member of the G-protein coupled receptor 1 family and encodes a multi-pass membrane protein. provided by RefSeq, Jul 2008
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Resources
"NM_005295" in other vectors (4)
SKU Description Size Price
RC213524L3 Lenti-ORF clone of GPR22 (Myc-DDK-tagged)-Human G protein-coupled receptor 22 (GPR22) 10 ug
$757.00
RC213524L4 Lenti-ORF clone of GPR22 (mGFP-tagged)-Human G protein-coupled receptor 22 (GPR22) 10 ug
$757.00
RG213524 GPR22 (tGFP-tagged) - Human G protein-coupled receptor 22 (GPR22) 10 ug
$489.00 $657.00
SC116801 GPR22 (untagged)-Human G protein-coupled receptor 22 (GPR22) 10 ug
$457.00

Plasmid Map for RC213524

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC213524
Circular map for RC213524

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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