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NFH (NEFH) (NM_021076) Human Tagged Lenti ORF Clone

  • LentiORF®
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SKU
RC213487L3
Lenti-ORF clone of NEFH (Myc-DDK-tagged)-Human neurofilament, heavy polypeptide (NEFH)
ORF Plasmid:
DDK DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
  $1,188.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol NFH
Synonyms CMT2CC; NFH
Vector pLenti-C-Myc-DDK-P2A-Puro
E. coli Selection Chloramphenicol (34 ug/mL)
Mammalian Cell Selection Puromycin
Sequence Data
ORF Nucleotide Sequence
The ORF insert of this clone is exactly the same as(RC213487).
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_021076
ORF Size 3060 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_021076.2
RefSeq Size 3695 bp
RefSeq ORF 3063 bp
Locus ID 4744
UniProt ID P12036
Cytogenetics 22q12.2
Domains filament
Protein Families Druggable Genome
Protein Pathways Amyotrophic lateral sclerosis (ALS)
MW 111.7 kDa
Summary Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and functionally maintain neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the heavy neurofilament protein. This protein is commonly used as a biomarker of neuronal damage and susceptibility to amyotrophic lateral sclerosis (ALS) has been associated with mutations in this gene. provided by RefSeq, Oct 2008
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Documents
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"NM_021076" in other vectors (4)
SKU Description Size Price
RC213487 NEFH (Myc-DDK-tagged)-Human neurofilament, heavy polypeptide (NEFH) 10 ug
$589.00 $888.00
RC213487L4 Lenti-ORF clone of NEFH (mGFP-tagged)-Human neurofilament, heavy polypeptide (NEFH) 10 ug
$1,188.00
RG213487 NEFH (tGFP-tagged) - Human neurofilament, heavy polypeptide (NEFH) 10 ug
$789.00 $1,088.00
SC110961 NEFH (untagged)-Human neurofilament, heavy polypeptide (NEFH) 10 ug
$889.00

Plasmid Map for RC213487L3

Download sequence for

file-download TXT (FASTA format)

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Cloning scheme for RC213487L3
Circular map for RC213487L3

file-alt Citations

A pan-cancer transcriptome analysis of exitron splicing identifies novel cancer driver genes and neoepitopes
Wang, Liu, Ren et al
Mol Cell (2021) 81 (10), 2246-2260.e12 DOI: 10.1016/j.molcel.2021.03.028
Cited by 80
AI Summary: In this study, OriGene products—including the Human NEFH wild type expressing vector (RC213487L3) and the pLenti-C-Myc-DDK-P2A-Puro vector (PS100092)—were used to functionally characterize the effects of exitron splicing on cancer driver genes.
Snippet: Human NEFH wide type expressing vector Origene RC213487L3 Human NEFH mutations expressing vector This paper N/A Human FOXA1 wide type and mutations expressing vector This paper N/A pLenti-C-Myc-DDK-P2A-Puro Origene PS100092 pGL3 Luciferase Reporter Vectors Promoter Promega Cat#E1761 pCR4Blunt-TOPO Vector Invitrogen Cat#460366 pRL-CMV Renilla Control Reporter Vectors Promega Cat# E2261
Showing 1-1 of 1 papers.
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