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NFH (NEFH) (NM_021076) Human Tagged ORF Clone

  • TrueORF®
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SKU
RC213487
NEFH (Myc-DDK-tagged)-Human neurofilament, heavy polypeptide (NEFH)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
$589.00 $888.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol NFH
Synonyms CMT2CC; NFH
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC213487 representing NM_021076
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGATGAGCTTCGGCGGCGCGGACGCGCTGCTGGGCGCCCCGTTCGCGCCGCTGCATGGCGGCGGCAGCC
TCCACTACGCGCTAGCCCGAAAGGGTGGCGCAGGCGGGACGCGCTCCGCCGCTGGCTCCTCCAGCGGCTT
CCACTCGTGGACACGGACGTCCGTGAGCTCCGTGTCCGCCTCGCCCAGCCGCTTCCGTGGCGCAGGCGCC
GCCTCAAGCACCGACTCGCTGGACACGCTGAGCAACGGGCCGGAGGGCTGCATGGTGGCGGTGGCCACCT
CACGCAGTGAGAAGGAGCAGCTGCAGGCGCTGAACGACCGCTTCGCCGGGTACATCGACAAGGTGCGGCA
GCTGGAGGCGCACAACCGCAGCCTGGAGGGCGAGGCTGCGGCGCTGCGGCAGCAGCAGGCGGGCCGCTCC
GCTATGGGCGAGCTGTACGAGCGCGAGGTCCGCGAGATGCGCGGCGCGGTGCTGCGCCTGGGCGCGGCGC
GCGGTCAGCTACGCCTGGAGCAGGAGCACCTGCTCGAGGACATCGCGCACGTGCGCCAGCGCCTAGACGA
CGAGGCCCGGCAGCGAGAGGAGGCCGAGGCGGCGGCCCGCGCGCTGGCGCGCTTCGCGCAGGAGGCCGAG
GCGGCGCGCGTGGACCTGCAGAAGAAGGCGCAGGCGCTGCAGGAGGAGTGCGGCTACCTGCGGCGCCAYC
ACCAGGAAGAGGTGGGCGAGCTGCTCGGCCAGATCCAGGGCTCCGGCGCCGCGCAGGCGCAGATGCAGGC
CGAGACGCGCGACGCCCTGAAGTGCGACGTGACGTCGGCGCTGCGCGAGATTCGCGCGCAGCTTGAAGGC
CACGCGGTGCAGAGCACGCTGCAGTCCGAGGAGTGGTTCCGAGTGAGGCTGGACCGACTGTCGGAGGCAG
CCAAGGTGAACACAGACGCTATGCGCTCAGCGCAGGAGGAGATAACTGAGTACCGGCGTCAGCTGCAGGC
CAGGACCACAGAGCTGGAGGCACTGAAAAGCACCAAGGACTCACTGGAGAGGCAGCGCTCTGAGCTGGAG
GACCGTCATCAGGCCGACATTGCCTCCTACCAGGAAGCCATTCAGCAGCTGGACGCTGAGCTGAGGAACA
CCAAGTGGGAGATGGCCGCCCAGCTGCGAGAATACCAGGACCTGCTCAATGTCAAGATGGCTCTGGATAT
AGAGATAGCCGCTTACAGAAAACTCCTGGAAGGTGAAGAGTGTCGGATTGGCTTTGGCCCAATTCCTTTC
TCGCTTCCAGAAGGACTCCCCAAAATTCCCTCTGTGTCCACTCACATAAAGGTGAAAAGCGAAGAGAAGA
TCAAAGTGGTGGAGAAGTCTGAGAAAGAAACTGTGATTGTGGAGGAACAGACAGAGGAGACCCAAGTGAC
TGAAGAAGTGACTGAAGAAGAGGAGAAAGAGGCCAAAGAGGAGGAGGGCAAGGAGGAAGAAGGGGGTGAA
GAAGAGGAGGCAGAAGGGGGAGAAGAAGAAACAAAGTCTCCCCCAGCAGAAGAGGCTGCATCCCCAGAGA
AGGAAGCCAAGTCACCAGTAAAGGAAGAGGCAAAGTCACCGGCTGAGGCCAAGTCCCCAGAGAAGGAGGA
AGCAAAATCCCCAGCCGAAGTCAAGTCCCCTGAGAAGGCCAAGTCTCCAGCAAAGGAAGAGGCAAAGTCA
CCGCCTGAGGCCAAGTCCCCAGAGAAGGAGGAAGCAAAATCTCCAGCTGAGGTCAAGTCCCCCGAGAAGG
CCAAGTCCCCAGCAAAGGAAGAGGCAAAGTCACCGGCTGAGGCCAAGTCTCCAGAGAAGGCCAAGTCCCC
AGTGAAGGAAGAAGCAAAGTCACTGGCTGAGGCCAAGTCCCCAGTGAAGGAAGAAGCAAAATCTCCAGCT
GAGGTCAAGTCCCCGGAAAAGGCCAAGTCTCCAACGAAGGAGGAAGCAAAGTCCCCTGAGAAGGCCAAGT
CCCCAGAGAAGGAAGAGGCCAAGTCCCCTGAGAAGGCCAAGTCCCCAGTGAAGGCAGAAGCAAAGTCCCC
TGAGAAGGCCAAGTCCCCAGTGAAGGCAGAAGCAAAGTCCCCTGAGAAGGCCAAGTCCCCAGTGAAGGAA
GAAGCAAAGTCCCCTGAGAAGGCCAAGTCCCCAGTGAAGGAAGAAGCAAAGTCCCCTGAGAAGGCCAAGT
CCCCAGTGAAGGAAGAAGCAAAGACCCCCGAGAAGGCCAAGTCCCCAGTGAAGGAAGAAGCTAAGTCCCC
AGAGAAGGCCAAGTCCCCAGAGAAGGCCAAGACTCTTGATGTGAAGTCTCCAGAAGCCAAGACTCCAGCG
AAGGAGGAAGCAAGGTCCCCTGCAGACAAATTCCCTGAAAAGGCCAAAAGCCCTGTCAAGGAGGAGGTCA
AGTCCCCAGAGAAGGCGAAATCTCCCCTGAAGGAGGATGCCAAGGCCCCTGAGAAGGAGATCCCAAAAAA
GGAAGAGGTGAAGTCCCCAGTGAAGGAGGAGGAGAAGCCCCAGGAGGTGAAAGTCAAAGAGCCCCCAAAG
AAGGCAGAGGAAGAGAAAGCCCCTGCCACACCAAAAACAGAGGAGAAGAAGGACAGCAAGAAAGAGGAGG
CACCCAAGAAGGAGGCTCCAAAGCCCAAGGTGGAGGAGAAGAAGGAACCTGCTGTCGAAAAGCCCAAAGA
ATCCAAAGTTGAAGCCAAGAAGGAAGAGGCTGAAGATAAGAAAAAAGTCCCCACCCCAGAGAAGGAGGCT
CCTGCCAAGGTGGAGGTGAAGGAAGACGCTAAACCCAAAGAAAAGACAGAGGTAGCCAAGAAGGAACCAG
ATGATGCCAAGGCCAAGGAACCCAGCAAACCAGCAGAGAAGAAGGAGGCAGCACCGGAGAAAAAAGACAC
CAAGGAGGAGAAGGCCAAGAAGCCTGAGGAGAAACCCAAGACAGAGGCCAAAGCCAAGGAAGATGACAAG
ACCCTCTCAAAAGAGCCTAGCAAGCCTAAGGCAGAAAAGGCTGAAAAATCCTCCAGCACAGACCAAAAAG
ACAGCAAGCCTCCAGAGAAGGCCACAGAAGACAAGGCCGCCAAGGGGAAG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC213487 representing NM_021076
Red=Cloning site Green=Tags(s)

MMSFGGADALLGAPFAPLHGGGSLHYALARKGGAGGTRSAAGSSSGFHSWTRTSVSSVSASPSRFRGAGA
ASSTDSLDTLSNGPEGCMVAVATSRSEKEQLQALNDRFAGYIDKVRQLEAHNRSLEGEAAALRQQQAGRS
AMGELYEREVREMRGAVLRLGAARGQLRLEQEHLLEDIAHVRQRLDDEARQREEAEAAARALARFAQEAE
AARVDLQKKAQALQEECGYLRRXHQEEVGELLGQIQGSGAAQAQMQAETRDALKCDVTSALREIRAQLEG
HAVQSTLQSEEWFRVRLDRLSEAAKVNTDAMRSAQEEITEYRRQLQARTTELEALKSTKDSLERQRSELE
DRHQADIASYQEAIQQLDAELRNTKWEMAAQLREYQDLLNVKMALDIEIAAYRKLLEGEECRIGFGPIPF
SLPEGLPKIPSVSTHIKVKSEEKIKVVEKSEKETVIVEEQTEETQVTEEVTEEEEKEAKEEEGKEEEGGE
EEEAEGGEEETKSPPAEEAASPEKEAKSPVKEEAKSPAEAKSPEKEEAKSPAEVKSPEKAKSPAKEEAKS
PPEAKSPEKEEAKSPAEVKSPEKAKSPAKEEAKSPAEAKSPEKAKSPVKEEAKSLAEAKSPVKEEAKSPA
EVKSPEKAKSPTKEEAKSPEKAKSPEKEEAKSPEKAKSPVKAEAKSPEKAKSPVKAEAKSPEKAKSPVKE
EAKSPEKAKSPVKEEAKSPEKAKSPVKEEAKTPEKAKSPVKEEAKSPEKAKSPEKAKTLDVKSPEAKTPA
KEEARSPADKFPEKAKSPVKEEVKSPEKAKSPLKEDAKAPEKEIPKKEEVKSPVKEEEKPQEVKVKEPPK
KAEEEKAPATPKTEEKKDSKKEEAPKKEAPKPKVEEKKEPAVEKPKESKVEAKKEEAEDKKKVPTPEKEA
PAKVEVKEDAKPKEKTEVAKKEPDDAKAKEPSKPAEKKEAAPEKKDTKEEKAKKPEEKPKTEAKAKEDDK
TLSKEPSKPKAEKAEKSSSTDQKDSKPPEKATEDKAAKGK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_021076
ORF Size 3060 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_021076.4
RefSeq Size 3695 bp
RefSeq ORF 3063 bp
Locus ID 4744
UniProt ID P12036
Cytogenetics 22q12.2
Domains filament
Protein Families Druggable Genome
Protein Pathways Amyotrophic lateral sclerosis (ALS)
MW 111.7 kDa
Summary Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and functionally maintain neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the heavy neurofilament protein. This protein is commonly used as a biomarker of neuronal damage and susceptibility to amyotrophic lateral sclerosis (ALS) has been associated with mutations in this gene. provided by RefSeq, Oct 2008
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"NM_021076" in other vectors (4)
SKU Description Size Price
RC213487L3 Lenti-ORF clone of NEFH (Myc-DDK-tagged)-Human neurofilament, heavy polypeptide (NEFH) 10 ug
$1,188.00
RC213487L4 Lenti-ORF clone of NEFH (mGFP-tagged)-Human neurofilament, heavy polypeptide (NEFH) 10 ug
$1,188.00
RG213487 NEFH (tGFP-tagged) - Human neurofilament, heavy polypeptide (NEFH) 10 ug
$789.00 $1,088.00
SC110961 NEFH (untagged)-Human neurofilament, heavy polypeptide (NEFH) 10 ug
$889.00

Plasmid Map for RC213487

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC213487
Circular map for RC213487

file-alt Citations

High serum prevalence of autoreactive IgG antibodies against peripheral nerve structures in patients with neurological post-COVID-19 vaccination syndrome
Arlt, Breuer, Trampenau et al
Front Immunol (2024) 15, 1404800 DOI: 10.3389/fimmu.2024.1404800
Cited by 10
AI Summary: OriGene products, specifically plasmids encoding Neurofilament heavy chain (NFH; #RC213487), Neurofilament medium chain (NFM; #RC224475), Neurofilament light chain (NFL; #RC205920), and Peripherin (PRPH; #RC207561), were used to identify and confirm autoantibody targets in patients with neurological post-COVID-19 vaccination syndrome.
Snippet: vity against neuronal intermediate filament antigens was adapted from previously published protocols (13). In brief, commercially available plasmids encoding Neurofilament heavy chain (NFH;#RC213487 Origene, Rockville, MD, USA), Neurofilament medium chain (NFM;#RC224475 Origene), Neurofilament light chain (NFL;#RC205920 Origene), Peripherin (PRPH;#RC207561 Origene), and Alpha-Internexin (INA
FigureFigure
Showing 1-1 of 1 papers.
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