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ANKRD20A1 (NM_032250) Human Tagged ORF Clone

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SKU
RC213475
ANKRD20A1 (Myc-DDK-tagged)-Human ankyrin repeat domain 20 family, member A1 (ANKRD20A1)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $754.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol ANKRD20A1
Synonyms ANKRD20A
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC213475 representing NM_032250
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAAGTTATTCGGCTTCGGGAGCCGCAGGGGCCAGACGGCCCAGGGCTCCATAGACCACGTCTACACGG
GTTCCGGATACCGAATCCGGGACTCCGAACTGCAGAAGATCCACAGGGCAGCTGTCAAAGGCGACGCCGC
GGAGGTGGAGCGCTGCCTGGCGCGCAGGAGCGGAGACCTGGACGCCCTGGACAAGCAGCACAGAACTGCT
CTACACTTGGCCTGTACCAGTGGCCATGTGCAAGTGGTCACTCTCCTGGTTAACAGAAAATGCCAGATTG
ATGTCTGTGACAAAGAAAACAGAACGCCTTTGATACAGGCTGTCCATTGCCAGGAAGAGGCTTGTGCCGT
TATTCTGCTGGAACATGGCGCCAATCCAAACCTTAAGGATATCTACGGCAACACTGCTCTCCATTATGCC
GTGTATAGTGAGAGCACCTCACTGGCAGAAAAACTGCTTTCCCATGGTGCACATATTGAAGCACTGGACA
AGGACAATAATACCCCACTTTTATTTGCTATAATTTGCAAGAAAGAGAAAATGGTGGAATTTTTATTGAA
AAAGAAAGCAAGTTCACATGCCGTTGATAGGCTGAGACGGTCAGCTCTCATGCTTGCTGTATACTATGAC
TCACCAGGTATTGTCAATATCCTTCTTAAGCAAAATATTGATGTCTTCGCTCAAGACATGTGTGGACGAG
ATGCAGAAGATTATGCTATTTCTCATCATTTGACAAAAATTCAACAACAAATTTTGGAACATAAAAAGAA
GATACTTAAAAAGGAGAAATCAGATGTTGGAAGTTCTGATGAATCTGCAGTCAGCATTTTCCATGAACTG
CGTGTGGATTCATTGCCTGCATCGGATGACAAAGACTTGAATGTTGCTACTAAGCAGTGTGTCCCCGAGA
AAGTGTCAGAGCCTTTACCTGGATCTTCGCATGAAAAAGGAAACAGAATAGTCAATGGACAAGGAGAAGG
GCCTCCTGCAAAACATCCTTCCTTGAAGCCTAGCACTGAAGTGGAAGATCCTGCTGTGAAAGGAGCAGTA
CAAAGAAAGAATGTACAGACATTGAGAGCAGAACAAGCCTTACCAGTGGCTTCAGAGGAAGAGCAAGAAA
GGCATGAAAGAAGTGAAAAGAAGCAACCACAGGTCAAAGAAGGAAATAATACAAACAAAAGTGAAAAAAT
ACAACTTTCAGAAAATATATGTGATAGTACATCTTCTGCTGCTGCTGGCAGATTAACCCAACAAAGAAAG
ATTGGGAAAACGTATCCTCAGCAATTTCCCAAGAAGCTGAAGGAAGAGCATGATAGATGCACCTTAAAAC
AAGAAAATGAAGAAAAAACAAATGTTAATATGCTGTACAAAAAAAATAGAGAAGAATTAGAAAGGAAAGA
GAAACAATATAAGAAAGAAGTTGAAGCAAAACAACTTGAACCAACTGTTCAGTCACTAGAGATGAAATCA
AAGACTGCAAGAAATACTCCAAATTGGGATTTTCATAATCATGAAGAAATGAAAGGTCTGATGGATGAAA
ATTGCATTTTGAAGGCAGATATTGCTATACTCAGACAGGAAATATGTACAATGAAAAATGACAACTTGGA
AAAAGAAAATAAATATCTTAAGGACATTAAAATTGTTAAAGAAACAAATGCTGCCCTTGAAAAGTATATA
AAACTCAATGAGGAAATGATAACAGAAACAGCATTCCGGTATCAACAAGAGCTTAATGATCTCAAGGCTG
AGAATACAAGGCTCAATGCCGAACTGTTGAAGGAAAAAGAAAGCAAGAAAAGACTGGAAGCTGACATTGA
ATCTTATCAGTCTAGACTGGCTGCTGCTATAAGCAAACACAGTGAAAGTGTGAAAACAGAAAGAAACCTA
AAACTTGCTTTAGAGAGAACACGAGATGTTTCTGTACAAGTAGAAATGAGTTCTGCTATTTCCAAAGTAA
AAGCTGAGAATGAGTTTCTTACTGAACAACTTTCTGAAACACAAATTAAATTCAATGCCTTAAAAGATAA
GTTCCGTAAGACAAGAGATAGTCTCAGAAAAAAGTCATTGGCTTTAGAAACTGTACAAAACGACCTAAGC
CAAACACAGCAGCAAACACAGGAAATGAAAGAGATGTATCAAAATGCAGAAGCTAAAGTGAATAATTCCA
CTGGAAAGTGGAACTGTGTAGAAGAGAGGATATGTCACCTCCAACGTGAAAATGCGTGGCTTGTACAGCA
ACTAGATGACGTTCATCAGAAAGAGGATCATAAAGAGATAGTAACTAATATCCAAAGAGGCTTTATTGAG
AGTGGAAAGAAAGACCTCGTGCTAGAAGAGAAAAGTAAGAAGCTAATGAATGAATGTGATCATTTAAAAG
AAAGTCTCTTTCAGTATGAGAGAGAGAAAACAGAAGGAGTAGTAAGTATCAAGGAAGATAAATATTTTCA
AACTTCTAGAAAGACAATT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC213475 representing NM_032250
Red=Cloning site Green=Tags(s)

MKLFGFGSRRGQTAQGSIDHVYTGSGYRIRDSELQKIHRAAVKGDAAEVERCLARRSGDLDALDKQHRTA
LHLACTSGHVQVVTLLVNRKCQIDVCDKENRTPLIQAVHCQEEACAVILLEHGANPNLKDIYGNTALHYA
VYSESTSLAEKLLSHGAHIEALDKDNNTPLLFAIICKKEKMVEFLLKKKASSHAVDRLRRSALMLAVYYD
SPGIVNILLKQNIDVFAQDMCGRDAEDYAISHHLTKIQQQILEHKKKILKKEKSDVGSSDESAVSIFHEL
RVDSLPASDDKDLNVATKQCVPEKVSEPLPGSSHEKGNRIVNGQGEGPPAKHPSLKPSTEVEDPAVKGAV
QRKNVQTLRAEQALPVASEEEQERHERSEKKQPQVKEGNNTNKSEKIQLSENICDSTSSAAAGRLTQQRK
IGKTYPQQFPKKLKEEHDRCTLKQENEEKTNVNMLYKKNREELERKEKQYKKEVEAKQLEPTVQSLEMKS
KTARNTPNWDFHNHEEMKGLMDENCILKADIAILRQEICTMKNDNLEKENKYLKDIKIVKETNAALEKYI
KLNEEMITETAFRYQQELNDLKAENTRLNAELLKEKESKKRLEADIESYQSRLAAAISKHSESVKTERNL
KLALERTRDVSVQVEMSSAISKVKAENEFLTEQLSETQIKFNALKDKFRKTRDSLRKKSLALETVQNDLS
QTQQQTQEMKEMYQNAEAKVNNSTGKWNCVEERICHLQRENAWLVQQLDDVHQKEDHKEIVTNIQRGFIE
SGKKDLVLEEKSKKLMNECDHLKESLFQYEREKTEGVVSIKEDKYFQTSRKTI

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_032250
ORF Size 2469 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_032250.5
RefSeq Size 3527 bp
RefSeq ORF 2472 bp
Locus ID 84210
UniProt ID Q5TYW2, A0A384NKR9
Cytogenetics 9q21.11
Domains ANK
MW 93.9 kDa
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Documents
Resources
"NM_032250" in other vectors (4)
SKU Description Size Price
RC213475L3 Lenti ORF clone of Human ankyrin repeat domain 20 family, member A1 (ANKRD20A1), Myc-DDK-tagged 10 ug
$1,054.00
RC213475L4 Lenti ORF clone of Human ankyrin repeat domain 20 family, member A1 (ANKRD20A1), mGFP tagged 10 ug
$1,054.00
RG213475 ANKRD20A1 (tGFP-tagged) - Human ankyrin repeat domain 20 family, member A1 (ANKRD20A1) 10 ug
$954.00
SC310092 ANKRD20A1 (untagged)-Human ankyrin repeat domain 20 family, member A1 (ANKRD20A1) 10 ug
$830.00

Plasmid Map for RC213475

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC213475
Circular map for RC213475

file-alt Citations

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