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A1CF (NM_014576) Human Tagged ORF Clone

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SKU
RC213415
A1CF (Myc-DDK-tagged)-Human APOBEC1 complementation factor (A1CF), transcript variant 1
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $545.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol A1CF
Synonyms ACF; ACF64; ACF65; APOBEC1CF; ASP
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC213415 representing NM_014576
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAATCAAATCACAAATCCGGGGATGGATTGAGCGGCACTCAGAAGGAAGCAGCCCTCCGCGCACTGG
TCCAGCGCACAGGATATAGCTTGGTCCAGGAAAATGGACAAAGAAAATATGGTGGCCCTCCACCTGGTTG
GGATGCTGCACCCCCTGAAAGGGGCTGTGAAATTTTTATTGGAAAACTTCCCCGAGACCTTTTTGAGGAT
GAGCTTATACCATTATGTGAAAAAATCGGTAAAATTTATGAAATGAGAATGATGATGGATTTTAATGGCA
ACAATAGAGGATATGCATTTGTAACATTTTCAAATAAAGTGGAAGCCAAGAATGCAATCAAGCAACTTAA
TAATTATGAAATTAGAAATGGGCGCCTCTTAGGGGTTTGTGCCAGTGTGGACAACTGCCGATTATTTGTT
GGGGGCATCCCAAAAACCAAAAAGAGAGAAGAAATCTTATCGGAGATGAAAAAGGTTACTGAAGGTGTTG
TCGATGTCATCGTCTACCCAAGCGCTGCAGATAAAACCAAAAACCGAGGCTTTGCCTTCGTGGAGTATGA
GAGTCATCGAGCAGCTGCCATGGCGAGGAGGAAACTGCTACCAGGAAGAATTCAGTTATGGGGACATGGT
ATTGCAGTAGACTGGGCAGAGCCAGAAGTAGAAGTTGATGAAGATACAATGTCTTCAGTGAAAATCCTAT
ATGTAAGAAATCTTATGCTGTCTACCTCTGAAGAGATGATTGAAAAGGAATTCAACAATATCAAACCAGG
TGCTGTGGAGAGGGTGAAGAAAATTCGAGACTATGCTTTTGTGCACTTCAGTAACCGAGAAGATGCAGTT
GAGGCTATGAAAGCTTTAAATGGCAAGGTGCTGGATGGTTCCCCCATTGAAGTCACCCTAGCAAAACCAG
TGGACAAGGACAGTTATGTTAGGTATACCCGAGGCACAGGTGGAAGGGGCACCATGCTGCAAGGAGAGTA
TACCTACTCTTTGGGCCAAGTTTATGATCCCACCACAACCTACCTTGGAGCTCCTGTCTTCTATGCCCCC
CAGACCTATGCAGCAATTCCCAGTCTTCATTTCCCAGCCACCAAAGGACATCTCAGCAACAGAGCCATTA
TCCGAGCCCCTTCTGTTAGAGGGGCTGCGGGAGTGAGAGGACTGGGCGGCCGTGGCTATTTGGCATACAC
AGGCCTGGGTCGAGGATACCAGGTCAAAGGAGACAAAAGAGAAGACAAACTCTATGACATTTTACCTGGG
ATGGAGCTCACCCCAATGAATCCTGTCACATTAAAACCCCAAGGAATTAAACTCGCTCCCCAGATATTAG
AAGAGATTTGTCAGAAAAATAACTGGGGACAGCCAGTGTACCAGCTGCACTCTGCTATTGGACAAGACCA
AAGACAGCTATTCTTGTACAAAATAACTATTCCTGCTCTAGCCAGCCAGAATCCTGCAATCCACCCTTTC
ACACCTCCAAAGCTGAGTGCCTTTGTGGATGAAGCAAAGACGTATGCAGCCGAATACACCCTGCAGACCC
TGGGCATCCCCACTGATGGAGGCGATGGCACCATGGCTACTGCTGCTGCTGCTGCTACTGCTTTCCCAGG
ATATGCTGTCCCTAATGCAACTGCACCCGTGTCTGCAGCCCAGCTCAAGCAAGCGGTAACCCTTGGACAA
GACTTAGCAGCATATACAACCTATGAGGTCTACCCAACTTTTGCAGTGACTGCCCGAGGGGATGGATATG
GCACCTTC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC213415 representing NM_014576
Red=Cloning site Green=Tags(s)

MESNHKSGDGLSGTQKEAALRALVQRTGYSLVQENGQRKYGGPPPGWDAAPPERGCEIFIGKLPRDLFED
ELIPLCEKIGKIYEMRMMMDFNGNNRGYAFVTFSNKVEAKNAIKQLNNYEIRNGRLLGVCASVDNCRLFV
GGIPKTKKREEILSEMKKVTEGVVDVIVYPSAADKTKNRGFAFVEYESHRAAAMARRKLLPGRIQLWGHG
IAVDWAEPEVEVDEDTMSSVKILYVRNLMLSTSEEMIEKEFNNIKPGAVERVKKIRDYAFVHFSNREDAV
EAMKALNGKVLDGSPIEVTLAKPVDKDSYVRYTRGTGGRGTMLQGEYTYSLGQVYDPTTTYLGAPVFYAP
QTYAAIPSLHFPATKGHLSNRAIIRAPSVRGAAGVRGLGGRGYLAYTGLGRGYQVKGDKREDKLYDILPG
MELTPMNPVTLKPQGIKLAPQILEEICQKNNWGQPVYQLHSAIGQDQRQLFLYKITIPALASQNPAIHPF
TPPKLSAFVDEAKTYAAEYTLQTLGIPTDGGDGTMATAAAAATAFPGYAVPNATAPVSAAQLKQAVTLGQ
DLAAYTTYEVYPTFAVTARGDGYGTF

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_014576
ORF Size 1758 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_014576.4
RefSeq Size 2128 bp
RefSeq ORF 1761 bp
Locus ID 29974
UniProt ID Q9NQ94, A0A024QZJ5
Cytogenetics 10q11.23
MW 64.1 kDa
Summary Mammalian apolipoprotein B mRNA undergoes site-specific C to U deamination, which is mediated by a multi-component enzyme complex containing a minimal core composed of APOBEC-1 and a complementation factor encoded by this gene. The gene product has three non-identical RNA recognition motifs and belongs to the hnRNP R family of RNA-binding proteins. It has been proposed that this complementation factor functions as an RNA-binding subunit and docks APOBEC-1 to deaminate the upstream cytidine. Studies suggest that the protein may also be involved in other RNA editing or RNA processing events. Several transcript variants encoding a few different isoforms have been found for this gene. provided by RefSeq, Nov 2010
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Documents
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"NM_014576" in other vectors (4)
SKU Description Size Price
RC213415L3 Lenti-ORF clone of A1CF (Myc-DDK-tagged)-Human APOBEC1 complementation factor (A1CF), transcript variant 1 10 ug
$845.00
RC213415L4 Lenti-ORF clone of A1CF (mGFP-tagged)-Human APOBEC1 complementation factor (A1CF), transcript variant 1 10 ug
$845.00
RG213415 A1CF (tGFP-tagged) - Human APOBEC1 complementation factor (A1CF), transcript variant 1 10 ug
$745.00
SC304130 A1CF (untagged)-Human APOBEC1 complementation factor (A1CF), transcript variant 1 10 ug
$601.00

Plasmid Map for RC213415

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC213415
Circular map for RC213415

file-alt Citations

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