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Glucosidase 2 subunit beta (PRKCSH) (NM_001001329) Human Tagged ORF Clone

  • TrueORF®
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SKU
RC213328
PRKCSH (Myc-DDK-tagged)-Human protein kinase C substrate 80K-H (PRKCSH), transcript variant 2
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $489.00
In Stock*
Bulk Requests & Clone Modifications

Frequently Bought Together (6)

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Other products for "Glucosidase 2 subunit beta or NM_001001329"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol Glucosidase 2 subunit beta
Synonyms AGE-R2; G19P1; GIIB; PCLD; PCLD1; PKCSH; PLD1; VASAP-60
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC213328 representing NM_001001329
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCTGTTGCCGCTGCTGCTGCTGCTACCCATGTGCTGGGCCGTGGAGGTCAAGAGGCCCCGGGGCGTCT
CCCTCACCAATCATCACTTCTACGATGAGTCCAAGCCTTTCACCTGCCTGGACGGTTCGGCCACCATCCC
ATTTGATCAGGTCAACGATGACTATTGCGACTGCAAAGATGGCTCTGACGAGCCAGGCACGGCTGCCTGT
CCTAATGGCAGCTTCCACTGCACCAACACTGGCTATAAGCCCCTGTATATCCCCTCCAACCGGGTCAACG
ATGGTGTTTGTGACTGCTGCGATGGAACAGACGAGTACAACAGCGGCGTCATCTGTGAGAACACCTGCAA
AGAGAAGGGCCGTAAGGAGAGAGAGTCCCTGCAGCAGATGGCCGAGGTCACCCGCGAAGGGTTCCGTCTG
AAGAAGATCCTTATTGAGGACTGGAAGAAGGCACGGGAGGAGAAGCAGAAAAAGCTCATTGAGCTACAGG
CTGGGAAGAAGTCTCTGGAAGACCAGGTGGAGATGCTGCGGACAGTGAAGGAGGAAGCTGAGAAGCCAGA
GAGAGAGGCCAAAGAGCAGCACCAGAAGCTGTGGGAAGAGCAGCTGGCTGCTGCCAAGGCCCAACAGGAG
CAGGAGCTGGCGGCTGATGCCTTCAAGGAGCTGGATGATGACATGGACGGGACGGTCTCGGTGACTGAGC
TGCAGACTCACCCGGAGCTGGACACAGATGGGGATGGGGCGTTGTCAGAAGCGGAAGCTCAGGCCCTCCT
CAGTGGGGACACACAGACAGACGCCACCTCTTTCTACGACCGCGTCTGGGCCGCCATCAGGGACAAGTAC
CGGTCCGAGGCACTGCCCACCGACCTTCCAGCACCTTCTGCCCCTGACTTGACGGAGCCCAAGGAGGAGC
AGCCGCCAGTGCCCTCGTCGCCCACAGAGGAGGAGGAGGAGGAGGAGGAGGAGGAGGAAGAAGAGGCTGA
AGAAGAGGAGGAGGAGGAGGATTCCGAGGTGCAGGGGGAGCAGCCCAAGCCGGCCAGCCCTGCTGAGGAA
GACAAAATGCCGCCCTACGACGAGCAGACGCAGGCCTTCATCGATGCTGCCCAGGAGGCCCGCAACAAGT
TCGAGGAGGCCGAGCGGTCGCTGAAGGACATGGAGGAGTCCATCAGGAACCTGGAGCAAGAGATTTCTTT
TGACTTTGGCCCCAACGGGGAGTTTGCTTACCTGTACAGCCAGTGCTACGAGCTCACCACCAACGAATAC
GTCTACCGCCTCTGCCCCTTCAAGCTTGTCTCGCAGAAACCCAAACTCGGGGGCTCTCCCACCAGCCTTG
GCACCTGGGGCTCATGGATTGGCCCCGACCACGACAAGTTCAGTGCCATGAAGTATGAGCAAGGCACGGG
CTGCTGGCAGGGCCCCAACCGCTCCACCACCGTGCGCCTCCTGTGCGGGAAAGAGACCATGGTGACCAGC
ACCACAGAGCCCAGTCGCTGCGAGTACCTCATGGAGCTGATGACGCCAGCCGCCTGCCCGGAGCCACCGC
CTGAAGCACCCACCGAAGACGACCATGACGAGCTC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC213328 representing NM_001001329
Red=Cloning site Green=Tags(s)

MLLPLLLLLPMCWAVEVKRPRGVSLTNHHFYDESKPFTCLDGSATIPFDQVNDDYCDCKDGSDEPGTAAC
PNGSFHCTNTGYKPLYIPSNRVNDGVCDCCDGTDEYNSGVICENTCKEKGRKERESLQQMAEVTREGFRL
KKILIEDWKKAREEKQKKLIELQAGKKSLEDQVEMLRTVKEEAEKPEREAKEQHQKLWEEQLAAAKAQQE
QELAADAFKELDDDMDGTVSVTELQTHPELDTDGDGALSEAEAQALLSGDTQTDATSFYDRVWAAIRDKY
RSEALPTDLPAPSAPDLTEPKEEQPPVPSSPTEEEEEEEEEEEEEAEEEEEEEDSEVQGEQPKPASPAEE
DKMPPYDEQTQAFIDAAQEARNKFEEAERSLKDMEESIRNLEQEISFDFGPNGEFAYLYSQCYELTTNEY
VYRLCPFKLVSQKPKLGGSPTSLGTWGSWIGPDHDKFSAMKYEQGTGCWQGPNRSTTVRLLCGKETMVTS
TTEPSRCEYLMELMTPAACPEPPPEAPTEDDHDEL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001001329
ORF Size 1575 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001001329.2
RefSeq Size 2253 bp
RefSeq ORF 1578 bp
Locus ID 5589
UniProt ID P14314
Cytogenetics 19p13.2
Protein Families Druggable Genome
MW 59 kDa
Summary This gene encodes the beta-subunit of glucosidase II, an N-linked glycan-processing enzyme in the endoplasmic reticulum. The encoded protein is an acidic phosphoprotein known to be a substrate for protein kinase C. Mutations in this gene have been associated with the autosomal dominant polycystic liver disease. Alternative splicing results in multiple transcript variants. provided by RefSeq, Jan 2014
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Documents
Resources
"NM_001001329" in other vectors (4)
SKU Description Size Price
RC213328L3 Lenti-ORF clone of PRKCSH (Myc-DDK-tagged)-Human protein kinase C substrate 80K-H (PRKCSH), transcript variant 2 10 ug
$789.00
RC213328L4 Lenti-ORF clone of PRKCSH (mGFP-tagged)-Human protein kinase C substrate 80K-H (PRKCSH), transcript variant 2 10 ug
$789.00
RG213328 PRKCSH (tGFP-tagged) - Human protein kinase C substrate 80K-H (PRKCSH), transcript variant 2 10 ug
$689.00
SC300164 PRKCSH (untagged)-Human protein kinase C substrate 80K-H (PRKCSH), transcript variant 2 10 ug
$539.00

Plasmid Map for RC213328

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC213328
Circular map for RC213328

file-alt Citations

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