B4GALNT2 (NM_153446) Human Tagged ORF Clone

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SKU
RC213266
B4GALNT2 (Myc-DDK-tagged)-Human beta-1,4-N-acetyl-galactosaminyl transferase 2 (B4GALNT2), transcript variant 1
  $790.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol B4GALNT2
Synonyms B4GALT; GALGT2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC213266 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGGAGCGCTGGCTTTTCCGTGGGAAAATTCCACGTGGAGGTGGCCTCTCGCGGCCGGGAATGTGTCT
CGGGGACGCCCGAGTGTGGGAATCGGCTCGGGAGTGCGGGCTTCGGGGATCTCTGCTTGGAACTCAGAGG
CGCTGACCCAGCCTGGGGCCCGTTTGCTGCCCACGGGAGGAGCCGCCGTCAGGGCTCGAGATTTCTGTGG
CTCCTCAAGATATTGGTCATAATCCTGGTACTTGGCATTGTTGGATTTATGTTCGGAAGCATGTTCCTTC
AAGCAGTGTTCAGCAGCCCCAAGCCAGAACTCCCAAGTCCTGCCCCGGGTGTCCAGAAGCTGAAGCTTCT
GCCTGAGGAACGTCTCAGGAACCTCTTTTCCTACGATGGAATCTGGCTGTTCCCGAAAAATCAGTGCAAA
TGTGAAGCCAACAAAGAGCAGGGAGGTTACAACTTTCAGGATGCCTATGGCCAGAGCGACCTCCCAGCGG
TGAAAGCGAGGAGACAGGCTGAATTTGAACACTTTCAGAGGAGAGAAGGGCTGCCCCGCCCACTGCCCCT
GCTGGTCCAGCCCAACCTCCCCTTTGGGTACCCAGTCCACGGAGTGGAGGTGATGCCCCTGCACACGGTT
CCCATCCCAGGCCTCCAGTTTGAAGGACCCGATGCCCCCGTCTATGAGGTCACCCTGACAGCTTCTCTGG
GGACACTGAACACCCTTGCTGATGTCCCAGACAGTGTGGTGCAGGGCAGAGGCCAGAAGCAGCTGATCAT
TTCTACCAGTGACCGGAAGCTGTTGAAGTTCATTCTTCAGCACGTGACATACACCAGCACGGGGTACCAG
CACCAGAAGGTAGACATAGTGAGTCTGGAGTCCAGGTCCTCAGTGGCCAAGTTTCCAGTGACCATCCGCC
ATCCTGTCATACCCAAGCTATACGACCCTGGACCAGAGAGGAAGCTCAGAAACCTGGTTACCATTGCTAC
CAAGACTTTCCTCCGCCCCCACAAGCTCATGATCATGCTCCGGAGTATTCGAGAGTATTACCCAGACTTG
ACCGTAATAGTGGCTGATGACAGCCAGAAGCCCCTGGAAATTAAAGACAACCACGTGGAGTATTACACTA
TGCCCTTTGGGAAGGGTTGGTTTGCTGGTAGGAACCTGGCCATATCTCAGGTCACCACCAAATACGTTCT
CTGGGTGGACGATGATTTTCTCTTCAACGAGGAGACCAAGATTGAGGTGCTGGTGGATGTCCTGGAGAAA
ACAGAACTGGACGTGGTAGGCGGCAGTGTGCTGGGAAATGTGTTCCAGTTTAAGTTGTTGCTGGAACAGA
GTGAGAATGGGGCCTGCCTTCACAAGAGGATGGGATTTTTCCAACCCCTGGATGGCTTCCCCAGCTGCGT
GGTGACCAGTGGCGTGGTCAACTTCTTCCTGGCCCACACGGAGCGACTCCAAAGAGTTGGCTTTGATCCC
CGCCTGCAACGAGTGGCTCACTCAGAATTCTTCATTGATGGGCTAGGGACCCTACTCGTGGGGTCATGCC
CAGAAGTGATTATAGGTCACCAGTCTCGGTCTCCAGTGGTGGACTCAGAACTGGCTGCCCTAGAGAAGAC
CTACAATACATACCGGTCCAACACCCTCACCCGGGTCCAGTTCAAGCTGGCCCTCCACTACTTCAAGAAC
CATCTCCAATGTGCCGCA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC213266 protein sequence
Red=Cloning site Green=Tags(s)

MGSAGFSVGKFHVEVASRGRECVSGTPECGNRLGSAGFGDLCLELRGADPAWGPFAAHGRSRRQGSRFLW
LLKILVIILVLGIVGFMFGSMFLQAVFSSPKPELPSPAPGVQKLKLLPEERLRNLFSYDGIWLFPKNQCK
CEANKEQGGYNFQDAYGQSDLPAVKARRQAEFEHFQRREGLPRPLPLLVQPNLPFGYPVHGVEVMPLHTV
PIPGLQFEGPDAPVYEVTLTASLGTLNTLADVPDSVVQGRGQKQLIISTSDRKLLKFILQHVTYTSTGYQ
HQKVDIVSLESRSSVAKFPVTIRHPVIPKLYDPGPERKLRNLVTIATKTFLRPHKLMIMLRSIREYYPDL
TVIVADDSQKPLEIKDNHVEYYTMPFGKGWFAGRNLAISQVTTKYVLWVDDDFLFNEETKIEVLVDVLEK
TELDVVGGSVLGNVFQFKLLLEQSENGACLHKRMGFFQPLDGFPSCVVTSGVVNFFLAHTERLQRVGFDP
RLQRVAHSEFFIDGLGTLLVGSCPEVIIGHQSRSPVVDSELAALEKTYNTYRSNTLTRVQFKLALHYFKN
HLQCAA

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_153446
ORF Size 1698 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_153446.1, NP_703147.1
RefSeq Size 2021 bp
RefSeq ORF 1701 bp
Locus ID 124872
UniProt ID Q8NHY0
Cytogenetics 17q21.32
Protein Families Transmembrane
MW 63.3 kDa
Summary B4GALNT2 catalyzes the last step in the biosynthesis of the human Sd(a) antigen through the addition of an N-acetylgalactosamine residue via a beta-1,4 linkage to a subterminal galactose residue substituted with an alpha-2,3-linked sialic acid. B4GALNT2 also catalyzes the last step in the biosynthesis of the Cad antigen (Montiel et al., 2003 PubMed 12678917).supplied by OMIM, Mar 2008
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Documents
Resources
"NM_153446" in other vectors (6)
SKU Description Size Price
RC213266L1 Lenti ORF clone of Human beta-1,4-N-acetyl-galactosaminyl transferase 2 (B4GALNT2), transcript variant 1, Myc-DDK-tagged 10 ug
$1,090.00
RC213266L2 Lenti ORF clone of Human beta-1,4-N-acetyl-galactosaminyl transferase 2 (B4GALNT2), transcript variant 1, mGFP tagged 10 ug
$1,090.00
RC213266L3 Lenti ORF clone of Human beta-1,4-N-acetyl-galactosaminyl transferase 2 (B4GALNT2), transcript variant 1, Myc-DDK-tagged 10 ug
$1,090.00
RC213266L4 Lenti ORF clone of Human beta-1,4-N-acetyl-galactosaminyl transferase 2 (B4GALNT2), transcript variant 1, mGFP tagged 10 ug
$1,090.00
RG213266 B4GALNT2 (tGFP-tagged) - Human beta-1,4-N-acetyl-galactosaminyl transferase 2 (B4GALNT2), transcript variant 1 10 ug
$990.00
SC306607 B4GALNT2 (untagged)-Human beta-1,4-N-acetyl-galactosaminyl transferase 2 (B4GALNT2), transcript variant 1 10 ug
$844.00

Plasmid Map for RC213266

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Cloning scheme for RC213266

Circular map for RC213266

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