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TRPC5 (NM_012471) Human Tagged Lenti ORF Clone

  • LentiORF®
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SKU
RC213238L3
Lenti ORF clone of Human transient receptor potential cation channel, subfamily C, member 5 (TRPC5), Myc-DDK-tagged
ORF Plasmid:
DDK DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
  $1,191.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol TRPC5
Synonyms PPP1R159; TRP5
Vector pLenti-C-Myc-DDK-P2A-Puro
E. coli Selection Chloramphenicol (34 ug/mL)
Mammalian Cell Selection Puromycin
Sequence Data
ORF Nucleotide Sequence
The ORF insert of this clone is exactly the same as(RC213238).
Restriction Sites SgfI-MluI Cloning Scheme for this gene |
ACCN NM_012471
ORF Size 2919 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_012471.1
RefSeq Size 5839 bp
RefSeq ORF 2922 bp
Locus ID 7224
UniProt ID Q9UL62
Cytogenetics Xq23
Domains ANK, ion_trans
Protein Families Druggable Genome, Ion Channels: Transient receptor potential, Transmembrane
MW 111.2 kDa
Summary This gene belongs to the transient receptor family. It encodes one of the seven mammalian TRPC (transient receptor potential channel) proteins. The encoded protein is a multi-pass membrane protein and is thought to form a receptor-activated non-selective calcium permeant cation channel. The protein is active alone or as a heteromultimeric assembly with TRPC1, TRPC3, and TRPC4. It also interacts with multiple proteins including calmodulin, CABP1, enkurin, Na(+)-H+ exchange regulatory factor (NHERF ), interferon-induced GTP-binding protein (MX1), ring finger protein 24 (RNF24), and SEC14 domain and spectrin repeat-containing protein 1 (SESTD1). provided by RefSeq, May 2010
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"NM_012471" in other vectors (6)
SKU Description Size Price
RC213238 TRPC5 (Myc-DDK-tagged)-Human transient receptor potential cation channel, subfamily C, member 5 (TRPC5) 10 ug
$891.00
RC213238L1 Lenti ORF clone of Human transient receptor potential cation channel, subfamily C, member 5 (TRPC5), Myc-DDK-tagged 10 ug
$1,191.00
RC213238L2 Lenti ORF clone of Human transient receptor potential cation channel, subfamily C, member 5 (TRPC5), mGFP tagged 10 ug
$1,191.00
RC213238L4 Lenti ORF clone of Human transient receptor potential cation channel, subfamily C, member 5 (TRPC5), mGFP tagged 10 ug
$1,191.00
RG213238 TRPC5 (tGFP-tagged) - Human transient receptor potential cation channel, subfamily C, member 5 (TRPC5) 10 ug
$789.00 $1,091.00
SC310023 TRPC5 (untagged)-Human transient receptor potential cation channel, subfamily C, member 5 (TRPC5) 10 ug
$892.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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