SPIN2B (NM_001006683) Human Tagged ORF Clone

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SKU
RC213224
SPIN2B (Myc-DDK-tagged)-Human spindlin family, member 2B (SPIN2B), transcript variant 3
  $300.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol SPIN2B
Synonyms dJ323P24.2; SPIN-2; SPIN-2B; SPIN2_duplicate; TDRD26
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC213224 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAAGACCCCCAACGCACAGGAAGCCGAAGGGCAACAAACCAGGGCAGCTGCAGGACGGGCCACTGGGT
CTGCAAACATGACAAAGAAAAAAGTCTCCCAAAAGAAGCAGAGAGGCCGACCTTCATCCCAGCCCCGCAG
GAACATCGTGGGCTGCAGAATTTCTCATGGATGGAAGGAAGGAGATGAGCCCATCACGCAGTGGAAAGGA
ACCGTTCTGGATCAGGTGCCTATAAATCCCTCTCTTTATCTGGTGAAATATGATGGAATTGACTGTGTCT
ATGGACTGGAACTTCACAGAGATGAAAGGGTTTTGTCTCTTAAAATTCTTTCTGACAGGGTGGCATCATC
TCACATTAGTGATGCCAACCTTGCAAATACCATAATTGGCAAAGCAGTGGAACATATGTTTGAGGGAGAG
CATGGTTCTAAGGATGAATGGAGGGGGATGGTCTTAGCTCAAGCACCTATCATGAAAGCCTGGTTTTATA
TTACCTATGAGAAAGATCCTGTCTTGTACATGTACCAGCTTCTAGATGATTATAAGGAAGGTGACCTCCG
CATCATGCCAGAATCCAGTGAGTCTCCTCCAACAGAGAGGGAGCCAGGAGGAGTTGTAGATGGCCTAATA
GGTAAGCATGTGGAATATACCAAAGAAGATGGCTCCAAAAGGATCGGCATGGTCATTCACCAAGTGGAAG
CCAAACCCTCTGTGTATTTCATCAAGTTTGATGATGATTTCCATATCTATGTCTACGATTTGGTGAAAAA
GTCC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC213224 protein sequence
Red=Cloning site Green=Tags(s)

MKTPNAQEAEGQQTRAAAGRATGSANMTKKKVSQKKQRGRPSSQPRRNIVGCRISHGWKEGDEPITQWKG
TVLDQVPINPSLYLVKYDGIDCVYGLELHRDERVLSLKILSDRVASSHISDANLANTIIGKAVEHMFEGE
HGSKDEWRGMVLAQAPIMKAWFYITYEKDPVLYMYQLLDDYKEGDLRIMPESSESPPTEREPGGVVDGLI
GKHVEYTKEDGSKRIGMVIHQVEAKPSVYFIKFDDDFHIYVYDLVKKS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001006683
ORF Size 774 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001006683.1, NP_001006684.1
RefSeq Size 1216 bp
RefSeq ORF 777 bp
Locus ID 474343
UniProt ID Q9BPZ2, A0A024R9Y9
Cytogenetics Xp11.21
MW 29.2 kDa
Summary Involved in the regulation of cell cycle progression, this activity is related to the inhibition of apoptosis following the removal of essential growth factors (PubMed:12145692). Exhibits H3K4me3-binding activity (PubMed:29061846).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_001006683" in other vectors (4)
SKU Description Size Price
RC213224L3 Lenti ORF clone of Human spindlin family, member 2B (SPIN2B), transcript variant 3, Myc-DDK-tagged 10 ug
$600.00
RC213224L4 Lenti ORF clone of Human spindlin family, member 2B (SPIN2B), transcript variant 3, mGFP tagged 10 ug
$600.00
RG213224 SPIN2B (tGFP-tagged) - Human spindlin family, member 2B (SPIN2B), transcript variant 3 10 ug
$500.00
SC301114 SPIN2B (untagged)-Human spindlin family, member 2B (SPIN2B), transcript variant 3 10 ug
$330.00

Plasmid Map for RC213224

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Cloning scheme for RC213224

Circular map for RC213224

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