CTNS (NM_004937) Human Tagged ORF Clone

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SKU
RC213151
CTNS (Myc-DDK-tagged)-Human cystinosis, nephropathic (CTNS), transcript variant 2
  $457.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol CTNS
Synonyms CTNS-LSB; PQLC4; SLC66A4
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC213151 representing NM_004937
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGATAAGGAATTGGCTGACTATTTTTATCCTTTTTCCCCTGAAGCTCGTAGAGAAATGTGAGTCAAGCG
TCAGCCTCACTGTTCCTCCTGTCGTAAAGCTGGAGAACGGCAGCTCGACCAACGTCAGCCTCACCCTGCG
GCCACCATTAAATGCAACCCTGGTGATCACTTTTGAAATCACATTTCGTTCCAAAAATATTACTATCCTT
GAGCTCCCCGATGAAGTTGTGGTGCCTCCTGGAGTGACAAACTCCTCTTTTCAAGTGACATCTCAAAATG
TTGGACAACTTACTGTTTATCTACATGGAAATCACTCCAATCAGACCGGCCCGAGGATACGCTTTCTTGT
GATCCGCAGCAGCGCCATTAGCATCATAAACCAGGTGATTGGCTGGATCTACTTTGTGGCCTGGTCCATC
TCCTTCTACCCTCAGGTGATCATGAATTGGAGGCGGAAAAGTGTCATTGGTCTGAGCTTCGACTTCGTGG
CTCTGAACCTGACAGGCTTCGTGGCCTACAGTGTATTCAACATCGGCCTCCTCTGGGTGCCCTACATCAA
GGAGCAGTTTCTCCTCAAATACCCCAACGGAGTGAACCCCGTGAACAGCAACGACGTCTTCTTCAGCCTG
CACGCGGTTGTCCTCACGCTGATCATCATCGTGCAGTGCTGCCTGTATGAGCGCGGTGGCCAGCGCGTGT
CCTGGCCTGCCATCGGCTTCCTGGTGCTCGCGTGGCTCTTCGCATTTGTCACCATGATCGTGGCTGCAGT
GGGAGTGATCACGTGGCTGCAGTTTCTCTTCTGCTTCTCCTACATCAAGCTCGCAGTCACGCTGGTCAAG
TATTTTCCACAGGCCTACATGAACTTTTACTACAAAAGCACTGAGGGCTGGAGCATTGGCAACGTGCTCC
TGGACTTCACCGGGGGCAGCTTCAGCCTCCTGCAGATGTTCCTCCAGTCCTACAACAACGACCAGTGGAC
GCTGATCTTCGGAGACCCAACCAAGTTTGGACTCGGGGTCTTCTCCATCGTCTTCGACGTCGTCTTCTTC
ATCCAGCACTTCTGTTTGTACAGAAAGAGACCGGGGTATGACCAGCTGAAC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC213151 representing NM_004937
Red=Cloning site Green=Tags(s)

MIRNWLTIFILFPLKLVEKCESSVSLTVPPVVKLENGSSTNVSLTLRPPLNATLVITFEITFRSKNITIL
ELPDEVVVPPGVTNSSFQVTSQNVGQLTVYLHGNHSNQTGPRIRFLVIRSSAISIINQVIGWIYFVAWSI
SFYPQVIMNWRRKSVIGLSFDFVALNLTGFVAYSVFNIGLLWVPYIKEQFLLKYPNGVNPVNSNDVFFSL
HAVVLTLIIIVQCCLYERGGQRVSWPAIGFLVLAWLFAFVTMIVAAVGVITWLQFLFCFSYIKLAVTLVK
YFPQAYMNFYYKSTEGWSIGNVLLDFTGGSFSLLQMFLQSYNNDQWTLIFGDPTKFGLGVFSIVFDVVFF
IQHFCLYRKRPGYDQLN

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_004937
ORF Size 1101 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_004937.1, NP_004928.1
RefSeq Size 2611 bp
RefSeq ORF 1104 bp
Locus ID 1497
UniProt ID O60931
Cytogenetics 17p13.2
Domains CTNS
Protein Families Druggable Genome, Transmembrane
Protein Pathways Lysosome
MW 41.6 kDa
Summary This gene encodes a seven-transmembrane domain protein that functions to transport cystine out of lysosomes. Its activity is driven by the H+ electrochemical gradient of the lysosomal membrane. Mutations in this gene cause cystinosis, a lysosomal storage disorder. Alternative splicing results in multiple transcript variants. provided by RefSeq, Jul 2009
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Documents
Resources
"NM_004937" in other vectors (4)
SKU Description Size Price
RC213151L3 Lenti ORF clone of Human cystinosis, nephropathic (CTNS), transcript variant 2, Myc-DDK-tagged 10 ug
$757.00
RC213151L4 Lenti ORF clone of Human cystinosis, nephropathic (CTNS), transcript variant 2, mGFP tagged 10 ug
$757.00
RG213151 CTNS (tGFP-tagged) - Human cystinosis, nephropathic (CTNS), transcript variant 2 10 ug
$489.00 $657.00
SC117040 CTNS (untagged)-Human cystinosis, nephropathic (CTNS), transcript variant 2 10 ug
$457.00

Plasmid Map for RC213151

Download sequence for

Cloning scheme for RC213151

Circular map for RC213151

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