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GFM2 (NM_170681) Human Tagged ORF Clone

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SKU
RC213111
GFM2 (Myc-DDK-tagged)-Human G elongation factor, mitochondrial 2 (GFM2), nuclear gene encoding mitochondrial protein, transcript variant 3
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $478.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol GFM2
Synonyms EF-G2mt; EFG2; hEFG2; mEF-G 2; MRRF2; MST027; MSTP027; RRF; RRF2; RRF2mt
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC213111 representing NM_170681
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTTGACCAACTTGAGGATATTTGCAATGAGTCATCAGACAATACCCAGTGTGTATATTAATAATATAT
GCTGCTATAAAATAAGAGCAAGTTTAAAAAGATTAAAGCCACATGTGCCGCTTGGAAGAAATTGCAGTTC
TCTACCAGGCTTAATAGGAAATGATATCAAATCCCTTCATTCCATCATCAATCCTCCCATAGCTAAAATC
CGTAATATTGGAATTATGGCTCATATTGATGCAGGCAAAACTACCACCACAGAAAGAATATTGTACTATT
CCGGATATACAAGATCACTGGGAGATGTTGATGATGGAGACACAGTGACAGATTTCATGGCCCAAGAGCG
AGAAAGAGGCATTACTATTCAATCAGCTGCTGTTACATTTGATTGGAAAGGTTATAGAGTCAATCTAATT
GATACACCAGGTCATGTGGACTTTACCTTGGAGGTTGAGCGGTGCCTAAGAGTGTTGGATGGTGCAGTGG
CTGTATTTGATGCCTCTGCTGGTGTAGAGGCCCAGACTCTCACAGTATGGAGGCAAGCTGATAAACACAA
TATACCTCGAATCTGTTTTTTAAACAAGATGGACAAAACTGGAGCAAGCTTTAAGTATGCAGTTGAAAGC
ATCAGAGAGAAGTTAAAGGCAAAGCCTTTGCTTTTACAGTTACCAATTGGTGAAGCCAAAACTTTCAAAG
GAGTGGTGGATGTAGTAATGAAAGAAAAACTTCTTTGGAATTGCAATTCAAATGATGGAAAAGACTTTGA
GAGAAAGCCCCTCTTGGAAATGAATGATCCTGAATTGCTGAAGGAAACAACTGAAGCAAGGAATGCCTTA
ATTGAACAAGTTGCAGATTTGGATGATGAATTTGCTGACTTGGTTTTAGAAGAATTTAGTGAGAATTTTG
ATTTGTTACCAGCTGAAAAGCTACAGACTGCAATACATAGAGTGACACTAGCTCAGACAGCAGTGCCTGT
GCTTTGTGGAAGTGCCCTGAAAAACAAAGGGATACAGCCCTTGTTAGATGCTGTTACTATGTACTTACCT
TCACCTGAAGAGCGTAACTATGAATTTCTGCAGTGGTATAAGGATGACTTATGTGCATTGGCATTTAAAG
TTCTCCATGACAAGCAGCGAGGACCACTGGTTTTTATGCGCATTTACTCAGGCACTATAAAACCCCAGTT
GGCCATTCATAATATTAATGGAAACTGCACGGAGAGAATAAGTCGTCTGCTTTTGCCGTTTGCTGACCAA
CATGTAGAAATCCCTTCATTGACTGCTGGTAACATTGCTTTGACTGTTGGGCTTAAACATACTGCCACTG
GAGACACCATTGTCTCATCCAAGTCCAGTGCATTAGCTGCAGCTCGTAGAGCCGAACGGGAGGGAGAAAA
GAAGCACAGACAAAACAATGAAGCAGAGAGACTTTTATTGGCTGGAGTGGAGATTCCAGAACCTGTTTTC
TTCTGTACCATAGAACCCCCATCACTGTCTAAGCAGCCAGGTATAAATGGTCTTAGTGTGTCAACTAAT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC213111 representing NM_170681
Red=Cloning site Green=Tags(s)

MLTNLRIFAMSHQTIPSVYINNICCYKIRASLKRLKPHVPLGRNCSSLPGLIGNDIKSLHSIINPPIAKI
RNIGIMAHIDAGKTTTTERILYYSGYTRSLGDVDDGDTVTDFMAQERERGITIQSAAVTFDWKGYRVNLI
DTPGHVDFTLEVERCLRVLDGAVAVFDASAGVEAQTLTVWRQADKHNIPRICFLNKMDKTGASFKYAVES
IREKLKAKPLLLQLPIGEAKTFKGVVDVVMKEKLLWNCNSNDGKDFERKPLLEMNDPELLKETTEARNAL
IEQVADLDDEFADLVLEEFSENFDLLPAEKLQTAIHRVTLAQTAVPVLCGSALKNKGIQPLLDAVTMYLP
SPEERNYEFLQWYKDDLCALAFKVLHDKQRGPLVFMRIYSGTIKPQLAIHNINGNCTERISRLLLPFADQ
HVEIPSLTAGNIALTVGLKHTATGDTIVSSKSSALAAARRAEREGEKKHRQNNEAERLLLAGVEIPEPVF
FCTIEPPSLSKQPGINGLSVSTN

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_170681
ORF Size 1539 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_170681.2, NP_733781.1
RefSeq Size 1962 bp
RefSeq ORF 1542 bp
Locus ID 84340
UniProt ID Q969S9
Cytogenetics 5q13.3
MW 56.9 kDa
Summary Eukaryotes contain two protein translational systems, one in the cytoplasm and one in the mitochondria. Mitochondrial translation is crucial for maintaining mitochondrial function and mutations in this system lead to a breakdown in the respiratory chain-oxidative phosphorylation system and to impaired maintenance of mitochondrial DNA. This gene encodes one of the mitochondrial translation elongation factors, which is a GTPase that plays a role at the termination of mitochondrial translation by mediating the disassembly of ribosomes from messenger RNA . Its role in the regulation of normal mitochondrial function and in disease states attributed to mitochondrial dysfunction is not known. Alternative splicing results in multiple transcript variants encoding distinct isoforms. provided by RefSeq, Jul 2013
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Documents
Resources
"NM_170681" in other vectors (4)
SKU Description Size Price
RC213111L3 Lenti-ORF clone of GFM2 (Myc-DDK-tagged)-Human G elongation factor, mitochondrial 2 (GFM2), nuclear gene encoding mitochondrial protein, transcript variant 3 10 ug
$778.00
RC213111L4 Lenti-ORF clone of GFM2 (mGFP-tagged)-Human G elongation factor, mitochondrial 2 (GFM2), nuclear gene encoding mitochondrial protein, transcript variant 3 10 ug
$778.00
RG213111 GFM2 (tGFP-tagged) - Human G elongation factor, mitochondrial 2 (GFM2), nuclear gene encoding mitochondrial protein, transcript variant 3 10 ug
$678.00
SC313793 GFM2 (untagged)-Human G elongation factor, mitochondrial 2 (GFM2), nuclear gene encoding mitochondrial protein, transcript variant 3 10 ug
$526.00

Plasmid Map for RC213111

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC213111
Circular map for RC213111

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