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PGAP2 (NM_014489) Human Tagged ORF Clone

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SKU
RC213084
PGAP2 (Myc-DDK-tagged)-Human post-GPI attachment to proteins 2 (PGAP2), transcript variant 1
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $300.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol PGAP2
Synonyms CWH43-N; FRAG1; HPMRS3; MRT17; MRT21
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC213084 representing NM_014489
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTACCAGGTCCCACTACCACTGGATCGGGATGGGACCCTGGTACGGCTCCGCTTCACCATGGTGGCCC
TGGTCACGGTCTGCTGTCCACTTGTCGCCTTCCTCTTCTGCATCCTCTGGTCCCTGCTCTTCCACTTCAA
GGAGACAACGGCCACACACTGTGGGGCCACGCCCTGCAGGATGTTCTCTGCGGCCTCCCAGCCTTTGGAC
CCCGATGGGACCTTGTTCCGGCTTCGCTTCACAGCCATGGTCTGGTGGGCCATCACTTTTCCTGTGTTCG
GCTTCTTCTTCTGCATCATCTGGTCCCTGGTGTTCCACTTTGAGTACACGGTGGCCACTGACTGTGGGGT
GCCCAATTACCTGCCCTCGGTGAGCTCAGCCATCGGCGGGGAGGTGCCCCAGCGCTACGTGTGGCGTTTC
TGCATCGGCCTGCACTCGGCGCCTCGCTTCTTGGTGGCCTTCGCCTACTGGAACCACTACCTCAGCTGCA
CCTCCCCGTGTTCCTGCTATCGCCCGCTCTGCCGCCTCAACTTCGGCCTCAATGTCGTGGAGAACCTCGC
GTTGCTAGTGCTCACTTATGTCTCCTCCTCCGAGGACTTCACCATCCACGAAAATGCTTTCATTGTGTTC
ATTGCCTCATCCCTCGGGCACATGCTCCTCACCTGCATTCTCTGGCGGTTGACCAAGAAGCACACAGTAA
GTCAGGAGGATCGCAAGTCCTACAGCTGGAAACAGCGGCTCTTCATCATCAACTTCATCTCCTTCTTCTC
GGCGCTGGCTGTCTACTTTCGGCACAACATGTATTGTGAGGCTGGAGTGTACACCATCTTTGCCATCCTG
GAGTACACTGTTGTCTTAACCAACATGGCGTTCCACATGACGGCCTGGTGGGACTTCGGGAACAAGGAGC
TGCTCATAACCTCTCAGCCTGAGGAAAAGCGATTC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC213084 representing NM_014489
Red=Cloning site Green=Tags(s)

MYQVPLPLDRDGTLVRLRFTMVALVTVCCPLVAFLFCILWSLLFHFKETTATHCGATPCRMFSAASQPLD
PDGTLFRLRFTAMVWWAITFPVFGFFFCIIWSLVFHFEYTVATDCGVPNYLPSVSSAIGGEVPQRYVWRF
CIGLHSAPRFLVAFAYWNHYLSCTSPCSCYRPLCRLNFGLNVVENLALLVLTYVSSSEDFTIHENAFIVF
IASSLGHMLLTCILWRLTKKHTVSQEDRKSYSWKQRLFIINFISFFSALAVYFRHNMYCEAGVYTIFAIL
EYTVVLTNMAFHMTAWWDFGNKELLITSQPEEKRF

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_014489
ORF Size 945 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_014489.4
RefSeq Size 2040 bp
RefSeq ORF 948 bp
Locus ID 27315
UniProt ID Q9UHJ9, A0A024RCC6
Cytogenetics 11p15.4
Protein Families Druggable Genome, Transmembrane
MW 36.2 kDa
Summary The protein encoded by this gene plays a role in the maturation of glycosylphosphatidylinositol (GPI) anchors on GPI-anchored proteins. Mutations in this gene are associated with an autosomal recessive syndrome characterized by hyperphosphatasia and intellectual disability. provided by RefSeq, Jul 2017
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"NM_014489" in other vectors (6)
SKU Description Size Price
RC213084L1 Lenti ORF clone of Human post-GPI attachment to proteins 2 (PGAP2), transcript variant 1, Myc-DDK-tagged 10 ug
$600.00
RC213084L2 Lenti ORF clone of Human post-GPI attachment to proteins 2 (PGAP2), transcript variant 1, mGFP tagged 10 ug
$600.00
RC213084L3 Lenti ORF clone of Human post-GPI attachment to proteins 2 (PGAP2), transcript variant 1, Myc-DDK-tagged 10 ug
$600.00
RC213084L4 Lenti ORF clone of Human post-GPI attachment to proteins 2 (PGAP2), transcript variant 1, mGFP tagged 10 ug
$600.00
RG213084 PGAP2 (tGFP-tagged) - Human post-GPI attachment to proteins 2 (PGAP2), transcript variant 1 10 ug
$500.00
SC312925 PGAP2 (untagged)-Human post-GPI attachment to proteins 2 (PGAP2), transcript variant 1 10 ug
$330.00

Plasmid Map for RC213084

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC213084
Circular map for RC213084

file-alt Citations

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