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TAF6 (NM_005641) Human Tagged ORF Clone

  • TrueORF®
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SKU
RC213036
TAF6 (Myc-DDK-tagged)-Human TAF6 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 80kDa (TAF6), transcript variant 1
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$589.00 $620.00
4 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol TAF6
Synonyms ALYUS; MGC:8964; TAF(II)70; TAF(II)80; TAF2E; TAFII-70; TAFII-80; TAFII70; TAFII80; TAFII85
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC213036 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTGAGGAGAAGAAGCTGAAGCTTAGCAACACTGTGCTGCCCTCGGAGTCCATGAAGGTGGTGGCTG
AATCCATGGGCATCGCCCAGATTCAGGAGGAGACCTGCCAGCTGCTAACGGATGAGGTCAGCTACCGCAT
CAAAGAGATCGCACAGGATGCCTTGAAGTTCATGCACATGGGGAAGCGGCAGAAGCTCACCACCAGTGAC
ATTGACTACGCCTTGAAGCTAAAGAATGTCGAGCCACTCTATGGCTTCCACGCCCAGGAGTTCATTCCTT
TCCGCTTCGCCTCTGGTGGGGGCCGGGAGCTTTACTTCTATGAGGAGAAGGAGGTTGATCTGAGCGACAT
CATCAATACCCCTCTGCCCCGGGTGCCCCTGGACGTCTGCCTCAAAGCTCATTGGCTGAGCATCGAGGGC
TGCCAGCCAGCTATCCCCGAGAACCCGCCCCCAGCTCCCAAAGAGCAACAGAAGGCTGAAGCCACAGAAC
CCCTGAAGTCAGCCAAGCCAGGCCAGGAGGAAGACGGACCCCTGAAGGGCAAAGGTCAAGGGGCCACCAC
AGCCGACGGCAAAGGGAAAGAGAAGAAGGCGCCGCCCTTGCTGGAGGGGGCCCCCTTGCGACTGAAGCCC
CGGAGCATCCACGAGTTGTCTGTGGAGCAGCAGCTCTACTACAAGGAGATCACCGAGGCCTGCGTGGGCT
CCTGCGAGGCCAAGAGGGCGGAAGCCCTGCAAAGCATTGCCACGGACCCTGGACTGTATCAGATGCTGCC
ACGGTTCAGTACCTTTATCTCGGAGGGGGTCCGTGTGAACGTGGTTCAGAACAACCTGGCCCTACTCATC
TACCTGATGCGTATGGTGAAAGCGCTGATGGACAACCCCACGCTCTATCTAGAAAAATACGTCCATGAGC
TGATTCCAGCTGTGATGACCTGCATCGTGAGCAGACAGTTGTGCCTGCGACCAGATGTGGACAATCACTG
GGCACTCCGAGACTTTGCTGCCCGCCTGGTGGCCCAGATCTGCAAGCATTTTAGCACAACCACTAACAAC
ATCCAGTCCCGGATCACCAAGACCTTCACCAAGAGCTGGGTGGACGAGAAGACGCCCTGGACGACTCGTT
ATGGCTCCATCGCAGGCTTGGCTGAGCTGGGACACGATGTTATCAAGACTCTGATTCTGCCCCGGCTGCA
GCAGGAAGGGGAGCGGATCCGCAGTGTGCTGGACGGCCCTGTGCTGAGCAACATTGACCGGATTGGAGCA
GACCATGTGCAGAGCCTCCTGCTGAAACACTGTGCTCCTGTTCTGGCAAAGCTGCGCCCACCGCCTGACA
ATCAGGACGCCTATCGGGCAGAATTCGGGTCCCTTGGGCCCCTCCTCTGCTCCCAGGTGGTCAAGGCTCG
GGCCCAGGCTGCTCTGCAGGCTCAGCAGGTCAACAGGACCACTCTGACCATCACGCAGCCCCGGCCCACG
CTGACCCTCTCGCAGGCCCCACAGCCTGGCCCTCGCACCCCTGGCTTGCTGAAGGTTCCTGGCTCCATCG
CACTTCCTGTCCAGACACTGGTGTCTGCACGAGCGGCTGCCCCACCACAGCCTTCCCCTCCTCCAACCAA
GTTTATTGTAATGTCATCGTCCTCCAGCGCCCCATCCACCCAGCAGGTCCTGTCCCTCAGCACCTCGGCC
CCCGGCTCAGGTTCCACCACCACTTCGCCCGTCACCACCACCGTCCCCAGCGTGCAGCCCATCGTCAAGT
TGGTCTCCACCGCCACCACCGCACCCCCCAGCACTGCTCCCTCTGGTCCTGGGAGTGTCCAGAAGTACAT
CGTGGTCTCACTTCCCCCAACAGGGGAGGGCAAAGGAGGCCCCACCTCCCATCCTTCTCCAGTTCCTCCC
CCGGCATCGTCCCCGTCCCCACTCAGCGGCAGTGCCCTTTGTGGGGGGAAGCAGGAGGCTGGGGACAGTC
CCCCTCCAGCTCCAGGGACTCCAAAAGCCAATGGCTCCCAGCCCAACTCCGGCTCCCCTCAGCCTGCTCC
G

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC213036 protein sequence
Red=Cloning site Green=Tags(s)

MAEEKKLKLSNTVLPSESMKVVAESMGIAQIQEETCQLLTDEVSYRIKEIAQDALKFMHMGKRQKLTTSD
IDYALKLKNVEPLYGFHAQEFIPFRFASGGGRELYFYEEKEVDLSDIINTPLPRVPLDVCLKAHWLSIEG
CQPAIPENPPPAPKEQQKAEATEPLKSAKPGQEEDGPLKGKGQGATTADGKGKEKKAPPLLEGAPLRLKP
RSIHELSVEQQLYYKEITEACVGSCEAKRAEALQSIATDPGLYQMLPRFSTFISEGVRVNVVQNNLALLI
YLMRMVKALMDNPTLYLEKYVHELIPAVMTCIVSRQLCLRPDVDNHWALRDFAARLVAQICKHFSTTTNN
IQSRITKTFTKSWVDEKTPWTTRYGSIAGLAELGHDVIKTLILPRLQQEGERIRSVLDGPVLSNIDRIGA
DHVQSLLLKHCAPVLAKLRPPPDNQDAYRAEFGSLGPLLCSQVVKARAQAALQAQQVNRTTLTITQPRPT
LTLSQAPQPGPRTPGLLKVPGSIALPVQTLVSARAAAPPQPSPPPTKFIVMSSSSSAPSTQQVLSLSTSA
PGSGSTTTSPVTTTVPSVQPIVKLVSTATTAPPSTAPSGPGSVQKYIVVSLPPTGEGKGGPTSHPSPVPP
PASSPSPLSGSALCGGKQEAGDSPPPAPGTPKANGSQPNSGSPQPAP

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_005641
ORF Size 2031 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_005641.4
RefSeq Size 2787 bp
RefSeq ORF 2034 bp
Locus ID 6878
UniProt ID P49848
Cytogenetics 7q22.1
Domains TAF
Protein Families Transcription Factors
Protein Pathways Basal transcription factors
MW 72.7 kDa
Summary Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes one of the smaller subunits of TFIID that binds weakly to TBP but strongly to TAF1, the largest subunit of TFIID. Alternative splicing results in multiple transcript variants. provided by RefSeq, Jun 2010
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Documents
Resources
"NM_005641" in other vectors (4)
SKU Description Size Price
RC213036L3 Lenti-ORF clone of TAF6 (Myc-DDK-tagged)-Human TAF6 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 80kDa (TAF6), transcript variant 1 10 ug
$920.00
RC213036L4 Lenti-ORF clone of TAF6 (mGFP-tagged)-Human TAF6 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 80kDa (TAF6), transcript variant 1 10 ug
$920.00
RG213036 TAF6 (tGFP-tagged) - Human TAF6 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 80kDa (TAF6), transcript variant 1 10 ug
$820.00
SC111695 TAF6 (untagged)-Human TAF6 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 80kDa (TAF6), transcript variant 1 10 ug
$851.00

Plasmid Map for RC213036

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC213036
Circular map for RC213036

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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