SOD2 (NM_001024465) Human Tagged ORF Clone
SKU
RC212924
SOD2 (Myc-DDK-tagged)-Human superoxide dismutase 2, mitochondrial (SOD2), nuclear gene encoding mitochondrial protein, transcript variant 2
$450.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | SOD2 |
| Synonyms | GClnc1; IPO-B; IPOB; Mn-SOD; MNSOD; MVCD6 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_001024465 |
| ORF Size | 666 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_001024465.3 |
| RefSeq Size | 1035 bp |
| RefSeq ORF | 669 bp |
| Locus ID | 6648 |
| UniProt ID | P04179, A0A384NL29 |
| Cytogenetics | 6q25.3 |
| Protein Families | Druggable Genome, Transcription Factors |
| Protein Pathways | Huntington's disease |
| MW | 24.8 kDa |
| Summary | This gene is a member of the iron/manganese superoxide dismutase family. It encodes a mitochondrial protein that forms a homotetramer and binds one manganese ion per subunit. This protein binds to the superoxide byproducts of oxidative phosphorylation and converts them to hydrogen peroxide and diatomic oxygen. Mutations in this gene have been associated with idiopathic cardiomyopathy (IDC), premature aging, sporadic motor neuron disease, and cancer. Alternative splicing of this gene results in multiple transcript variants. A related pseudogene has been identified on chromosome 1. provided by RefSeq, Apr 2016 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_001024465" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC212924L1 | Lenti ORF clone of Human superoxide dismutase 2, mitochondrial (SOD2), nuclear gene encoding mitochondrial protein, transcript variant 2, Myc-DDK-tagged | 10 ug |
$750.00
|
|
| RC212924L2 | Lenti ORF clone of Human superoxide dismutase 2, mitochondrial (SOD2), nuclear gene encoding mitochondrial protein, transcript variant 2, mGFP tagged | 10 ug |
$750.00
|
|
| RC212924L3 | Lenti ORF clone of Human superoxide dismutase 2, mitochondrial (SOD2), nuclear gene encoding mitochondrial protein, transcript variant 2, Myc-DDK-tagged | 10 ug |
$750.00
|
|
| RC212924L4 | Lenti ORF clone of Human superoxide dismutase 2, mitochondrial (SOD2), nuclear gene encoding mitochondrial protein, transcript variant 2, mGFP tagged | 10 ug |
$750.00
|
|
| RG212924 | SOD2 (tGFP-tagged) - Human superoxide dismutase 2, mitochondrial (SOD2), nuclear gene encoding mitochondrial protein, transcript variant 2 | 10 ug |
$650.00
|
|
| SC302252 | SOD2 (untagged)-Human superoxide dismutase 2, mitochondrial (SOD2), nuclear gene encoding mitochondrial protein, transcript variant 2 | 10 ug |
$330.00
|
|
Citations
Mitochondria-targeted paraquat and metformin mediate ROS production to induce multiple pathways of retrograde signaling: A dose-dependent phenomenon
Chowdhury, Zielonka, Kalyanaraman et al
Redox Biol (2020) 36, 101606 DOI: 10.1016/j.redox.2020.101606
Chowdhury, Zielonka, Kalyanaraman et al
Redox Biol (2020) 36, 101606 DOI: 10.1016/j.redox.2020.101606
Cited by 168
AI Summary: OriGene’s pCMV6 mammalian expression vector carrying human superoxide dismutase-2 (SOD2) cDNA (CAT#: RC212924) and its empty vector control were used to generate SOD2-overexpressing stable cell lines, enabling the study of mitochondrial ROS modulation in the reported experiments.
Snippet: 0% of oxygen and 5% CO2 (BOC gases, Murray Hill, NJ) [30]. 2.2 Generation of SOD2 expressing stable cell lines A pCMV6 mammalian expression vector carrying human superoxide dismutase-2 (sod2) cDNA (OriGene Technologies, Inc., CAT#: RC212924) and empty vector (control) were used to generate the overexpressing cell lines. … GENERATION OF SOD2 EXPRESSING STABLE CELL LINES A pCMV6 mammalian expression vector carrying human superoxide dismutase-2 (sod2) cDNA (OriGene Technologies, Inc., CAT#: RC212924) and empty vector (control) were used to generate the overexpressing cell lines. Raw264.7 cells were transfected using FuGENE 6 (Roche Diagnostics, Indianap;- Generation of SOD2 expressing stable cell lines. A pCMV6 mammalian expression vector carrying human superoxide dismutase-2 (sod2) cDNA (OriGene Technologies, Inc., CAT#: RC212924) and empty vector (control) were used to generate the overexpressing cell
Showing 1-1 of 1 papers.
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