LRRC37A (NM_014834) Human Tagged ORF Clone

  • TrueORF®
SKU
RC212905
LRRC37A (Myc-DDK-tagged)-Human leucine rich repeat containing 37A (LRRC37A)
  $1,428.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol LRRC37A
Synonyms LRRC37
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_014834
ORF Size 5100 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq Size 5177 bp
RefSeq ORF 5103 bp
Locus ID 9884
UniProt ID A6NMS7
Cytogenetics 17q21.31
Protein Families Transmembrane
MW 188.1 kDa
Reviews
 
 
 
 
 

Be the first to review this product

Please note:
Only reviews with images are eligible for a $20/20€ Amazon gift card.
Reviews without images are eligible for a $10/10€ Amazon gift card.
For more details about the terms and conditions, please visit the promotion page.

Review image
Documents
Resources
"NM_014834" in other vectors (3)
SKU Description Size Price
RG212905 LRRC37A (tGFP-tagged) - Human leucine rich repeat containing 37A (LRRC37A) 10 ug
$1,628.00
SC125622 LRRC37A (untagged)-Human leucine rich repeat containing 37A (LRRC37A) 10 ug
$1,374.00
SC318655 LRRC37A (untagged)-Human leucine rich repeat containing 37A (LRRC37A) 10 ug
$1,572.00

Plasmid Map for RC212905

Download sequence for

Cloning scheme for RC212905

Circular map for RC212905

file-alt Citations

17q21.31 sub-haplotypes underlying H1-associated risk for Parkinson's disease are associated with LRRC37A/2 expression in astrocytes
Bowles, Pugh, Liu et al
Mol Neurodegener (2022) 17 (1), 48 DOI: 10.1186/s13024-022-00551-x
Cited by 61
AI Summary: OriGene products, specifically the LRRC37A plasmid and empty vector control, were used to overexpress LRRC37A/2 in HEK293T cells to investigate its role in Parkinson's disease risk and astrocyte function.
Snippet: … For LRRC37A/2 overexpression experiments, HEK293T cells were seeded at a density of 1.4 × 105 cells per well in 6-well plates and transfected with 0.5–2.5ug of LRRC37A plasmid (Origene) or empty vector control (Origene) using Lipofectamine 3000. … For qRTPCR, protein biochemistry and LRRC37A2 knockdown experiments, iPSC lines (Ta;For LRRC37A/2 overexpression experiments, HEK293T cells were seeded at a density of 1.4 × 105 cells per well in 6-well plates and transfected with 0.5–2.5ug of LRRC37A plasmid (Origene) or empty vector control (Origene) using Lipofectamine 3000. …
FigureFigureFigure
17q21. 31 sub-haplotypes underlying H1-associated risk for Parkinson's disease and progressive supranuclear palsy converge on altered glial regulation
Bowles, Pugh, Farrell et al
bioRxiv (2019)
Cited by 3
Snippet: …For LRRC37A overexpression experiments, HEK293T cells were seeded at a density of 1.4x105 cells per well in 6-well plates and transfected with 0.5-2.5ug of LRRC37A plasmid (Origene) or empty vector control (Origene) using Lipofectamine 3000. …
Showing 1-2 of 2 papers.
Powered by BizGenius
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Related Products