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LRIG2 (NM_014813) Human Tagged ORF Clone

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SKU
RC212836
LRIG2 (Myc-DDK-tagged)-Human leucine-rich repeats and immunoglobulin-like domains 2 (LRIG2)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
  $927.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol LRIG2
Synonyms LIG-2; LIG2; UFS2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC212836 representing NM_014813
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGCCGGCGCCCCTAGGCGTCCCGGAGGAGCAGTTGCTGGGGTGTCGATCTAGAGTGCTTTCTCGGT
TACTCTTCATTGCCCAGACCGCTCTCCTCCTGTTGCCCGCCGCCGGAGCAGGTCTCTGCCCCGCGCCCTG
CTCCTGCCGCATTCCTCTCCTGGACTGCAGTCGCAGGAAATTGCCCGCACCGAGCTGGAGGGCGCTGTCG
GGCTTGCTGCCCCCCGACACCGCTATCCTGGATTTCAGTCATAATCGGTTGTCTAACTGGAACATCAGCT
TGGAATCACAAACATTACAGGAAGTGAAAATGAATTACAATGAACTAACAGAAATCCCGTATTTTGGAGA
ACCTACATCTAATATTACTCTACTTTCATTAGTCCATAATATAATCCCAGAAATAAATGCACAGGCACTC
CAGTTTTACCCTGCTCTGGAGAGTTTAGACCTCAGCTCAAATATAATATCAGAAATCAAGACATCTTCAT
TTCCTCGCATGCAGCTTAAATACCTGAATTTAAGTAATAACAGAATAACCACCTTGGAGGCTGGTTGCTT
CGATAATTTATCAAGTTCCTTATTAGTGGTAAAGTTAAACCGTAACCGAATGAGCATGATTCCACCTAAG
ATCTTCAAGCTGCCTCACCTCCAATTCTTGGAACTTAAAAGAAACAGAATTAAAATTGTGGAAGGTCTTA
CATTCCAAGGGCTTGACTCCTTAAGATCTTTGAAAATGCAGCGGAATGGAATTAGCAAACTTAAGGATGG
AGCATTTTTTGGCTTGAATAACATGGAAGAACTAGAACTGGAACACAACAACCTTACACGAGTAAACAAG
GGGTGGTTGTATGGCTTGCGAATGTTACAGCAGCTCTATGTGAGCCAGAATGCTATTGAAAGAATCAGCC
CTGATGCATGGGAGTTCTGCCAAAGACTATCCGAACTTGATTTGTCCTATAACCAGCTGACCCGCCTGGA
TGAATCTGCCTTTGTGGGTCTGAGCTTATTGGAGAGATTGAATTTAGGAGACAACAGAGTCACTCATATT
GCTGATGGTGTATTTAGATTTCTTTCCAATCTTCAGACATTAGACTTAAGAAACAATGAAATTTCATGGG
CCATAGAAGATGCTAGTGAAGCCTTTGCTGGACTCACAAGTCTCACTAAACTAATCTTACAAGGAAACCA
GATTAAGTCAATTACAAAGAAAGCATTCATTGGTCTTGAATCCCTTGAGCATCTAGATTTGAACAATAAT
GCTATAATGTCTATCCAAGAAAATGCTTTTTCTCAGACTCATTTAAAAGAACTGATTCTGAACACAAGCA
GTTTGCTCTGTGACTGCCATTTGAAGTGGCTACTTCAATGGTTGGTTGATAATAACTTTCAACATTCTGT
GAATGTAAGCTGTGCACACCCTGAATGGCTAGCAGGGCAAAGCATCCTGAATGTGGATCTGAAAGATTTT
GTCTGTGATGATTTTCTCAAGCCACAGATAAGGACACATCCTGAAACCATAATTGCTCTAAGAGGCATGA
ATGTGACTCTGACGTGCACTGCAGTGAGCAGCAGTGATTCACCCATGTCCACTGTGTGGCGCAAAGACAG
TGAAATCCTGTATGACGTGGATACTGAGAATTTTGTTCGTTATTGGCAGCAAGCTGGAGAAGCTCTGGAA
TATACTAGTATCTTACATCTTTTCAATGTGAATTTCACAGATGAAGGAAAATATCAGTGTATTGTTACTA
ATCACTTTGGTTCTAATTATTCTCAGAAAGCCAAACTGACTGTAAATGAGATGCCATCTTTTCTGAAAAC
GCCAATGGATCTGACTATTCGCACTGGTGCCATGGCCAGATTAGAATGTGCTGCAGAGGGACACCCTGCA
CCTCAGATTTCCTGGCAGAAAGATGGTGGTACTGACTTTCCTGCGGCTCGAGAAAGACGCATGCACGTCA
TGCCCGAGGATGACGTCTTCTTTATTGCCAATGTGAAAATAGAAGATATGGGAATCTATAGCTGCATGGC
ACAAAATACAGCAGGAGGTCTCTCAGCAAATGCTTCCCTAACAGTGTTAGAGACACCCTCATTTATTAGA
CCCCTGGAGGATAAGACAGTAACCCGAGGTGAAACTGCGGTGTTACAGTGCATAGCTGGAGGGAGTCCTG
CCCCTCGTCTCAACTGGACTAAAGATGATGGGCCTTTGCTGGTGACAGAACGACATTTCTTTGCTGCAGC
CAATCAGCTTCTCATCATTGTAGATGCCGGGCTAGAAGATGCTGGGAAATATACCTGCATTATGTCTAAC
ACCCTTGGGACAGAACGTGGCCACATTTACCTAAATGTCATTTCATCCCCCAATTGTGACTCTTCCCAGA
GTAGCATTGGGCATGAAGATGATGGCTGGACCACAGTTGGCATTGTCATCATTGTTGTGGTCTGCTGTGT
TGTTGGCACTTCTTTGATCTGGGTCATTGTTATTTACCACATGAGAAGGAAAAATGAAGACTATAGTATC
ACAAACACAGAGGAGCTCAATCTGCCTGCAGACATTCCCAGCTACTTGTCTTCCCAAGGAACGCTGTCTG
AGCCACAGGAAGGCTACAGCAACTCTGAGGCAGGCAGTCATCAGCAACTTATGCCTCCTGCCAATGGATA
TATACACAAAGGCACTGACGGTGGCACTGGTACCCGGGTGATTTGCTCAGATTGTTATGACAATGCCAAC
ATCTACTCCAGGACCCGAGAATACTGTCCATACACCTATATTGCTGAGGAGGACGTTCTTGATCAGACAC
TGTCCAGCCTCATGGTCCAAATGCCTAAAGAGACATATTTAGTACATCCTCCCCAGGATACTACTGCCCT
AGAGAGCCTGATACCGTCAGCCAACAGAGAGCCATCTGCCTTTCCCACCAACCATGAGAGGATAAGTGAG
AAGAAACTTCCCTCCACACAGATGAGCGGTGAAACATTGCAGCGGCCCGTGTGGAACATAAACAGAGAAC
TAGGCCTGCCTCATCCTCCTTTTTCCCAGCAGCCAGTCCATGAGTCACCACAACTTCATCAAAATGAGGG
CCTGGCAGGGAGAGAGCCAGACTGTTCTGCTTCTTCCATGTCCTGCCACAGGTTACAGGATCATGCTTTT
GATTTTAGTAGGACTCGGAACATTCAAGATGGTAGTGAGGGCACA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC212836 representing NM_014813
Red=Cloning site Green=Tags(s)

MAPAPLGVPEEQLLGCRSRVLSRLLFIAQTALLLLPAAGAGLCPAPCSCRIPLLDCSRRKLPAPSWRALS
GLLPPDTAILDFSHNRLSNWNISLESQTLQEVKMNYNELTEIPYFGEPTSNITLLSLVHNIIPEINAQAL
QFYPALESLDLSSNIISEIKTSSFPRMQLKYLNLSNNRITTLEAGCFDNLSSSLLVVKLNRNRMSMIPPK
IFKLPHLQFLELKRNRIKIVEGLTFQGLDSLRSLKMQRNGISKLKDGAFFGLNNMEELELEHNNLTRVNK
GWLYGLRMLQQLYVSQNAIERISPDAWEFCQRLSELDLSYNQLTRLDESAFVGLSLLERLNLGDNRVTHI
ADGVFRFLSNLQTLDLRNNEISWAIEDASEAFAGLTSLTKLILQGNQIKSITKKAFIGLESLEHLDLNNN
AIMSIQENAFSQTHLKELILNTSSLLCDCHLKWLLQWLVDNNFQHSVNVSCAHPEWLAGQSILNVDLKDF
VCDDFLKPQIRTHPETIIALRGMNVTLTCTAVSSSDSPMSTVWRKDSEILYDVDTENFVRYWQQAGEALE
YTSILHLFNVNFTDEGKYQCIVTNHFGSNYSQKAKLTVNEMPSFLKTPMDLTIRTGAMARLECAAEGHPA
PQISWQKDGGTDFPAARERRMHVMPEDDVFFIANVKIEDMGIYSCMAQNTAGGLSANASLTVLETPSFIR
PLEDKTVTRGETAVLQCIAGGSPAPRLNWTKDDGPLLVTERHFFAAANQLLIIVDAGLEDAGKYTCIMSN
TLGTERGHIYLNVISSPNCDSSQSSIGHEDDGWTTVGIVIIVVVCCVVGTSLIWVIVIYHMRRKNEDYSI
TNTEELNLPADIPSYLSSQGTLSEPQEGYSNSEAGSHQQLMPPANGYIHKGTDGGTGTRVICSDCYDNAN
IYSRTREYCPYTYIAEEDVLDQTLSSLMVQMPKETYLVHPPQDTTALESLIPSANREPSAFPTNHERISE
KKLPSTQMSGETLQRPVWNINRELGLPHPPFSQQPVHESPQLHQNEGLAGREPDCSASSMSCHRLQDHAF
DFSRTRNIQDGSEGT

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_014813
ORF Size 3195 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_014813.1, NP_055628.1
RefSeq Size 4015 bp
RefSeq ORF 3198 bp
Locus ID 9860
UniProt ID O94898
Cytogenetics 1p13.2
Domains ig, IG, IGc2, LRR, LRRCT, LRR_PS, LRR_SD22, LRR_TYP
Protein Families Transmembrane
MW 118.8 kDa
Summary This gene encodes a transmembrane protein containing leucine-rich repeats and immunoglobulin-like domains. The encoded protein promotes epidermal growth factor signalling, resulting in increased proliferation. Its expression in the cytoplasm of glioma cells is correlated with poor survival. Mutations in this gene can cause urofacial syndrome. Alternative splicing results in multiple transcript variants. provided by RefSeq, Aug 2015
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"NM_014813" in other vectors (6)
SKU Description Size Price
RC212836L1 Lenti-ORF clone of LRIG2 (Myc-DDK-tagged)-Human leucine-rich repeats and immunoglobulin-like domains 2 (LRIG2) 10 ug
$1,227.00
RC212836L2 Lenti-ORF clone of LRIG2 (mGFP-tagged)-Human leucine-rich repeats and immunoglobulin-like domains 2 (LRIG2) 10 ug
$1,227.00
RC212836L3 Lenti-ORF clone of LRIG2 (Myc-DDK-tagged)-Human leucine-rich repeats and immunoglobulin-like domains 2 (LRIG2) 10 ug
$1,227.00
RC212836L4 Lenti-ORF clone of LRIG2 (mGFP-tagged)-Human leucine-rich repeats and immunoglobulin-like domains 2 (LRIG2) 10 ug
$1,227.00
RG212836 LRIG2 (tGFP-tagged) - Human leucine-rich repeats and immunoglobulin-like domains 2 (LRIG2) 10 ug
$789.00 $1,127.00
SC315130 LRIG2 (untagged)-Human leucine-rich repeats and immunoglobulin-like domains 2 (LRIG2) 10 ug
$928.00

Plasmid Map for RC212836

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC212836
Circular map for RC212836

file-alt Citations

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