LGICZ1 (ZACN) (NM_180990) Human Tagged ORF Clone

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SKU
RC212824
ZACN (Myc-DDK-tagged)-Human zinc activated ligand-gated ion channel (ZACN)
  $457.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol LGICZ1
Synonyms L2; LGICZ; LGICZ1; ZAC; ZAC1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC212824 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCCTATGGTCCCTGCTCCATCTCACCTTCCTGGGGTTCAGCATTACCTTGCTGTTGGTCCACGGGC
AGGGCTTCCAAGGGACAGCAGCCATCTGGCCATCCCTCTTCAACGTCAACTTGTCCAAGAAGGTTCAGGA
AAGCATCCAGATCCCGAACAATGGGAGTGCGCCCCTGCTCGTGGATGTGCGGGTGTTTGTCTCCAACGTG
TTTAATGTGGACATCCTGCGATACACAATGTCCTCCATGCTGCTGCTTAGGCTGTCCTGGCTGGACACTC
GCCTGGCCTGGAACACTAGTGCACACCCGCGGCACGCCATCACGCTGCCCTGGGAGTCTCTCTGGACACC
AAGGCTCACCATCCTGGAGGCGCTCTGGGTGGACTGGAGGGACCAGAGCCCCCAGGCTCGAGTAGACCAG
GACGGCCACGTGAAGCTCAACCTGGCCCTCACCACGGAGACCAACTGCAACTTTGAGCTCCTCCACTTCC
CCCGGGACCACAGCAACTGCAGCCTCAGCTTCTACGCTCTCAGCAACACGGCGATGGAGTTAGAGTTCCA
GGCCCACGTGGTGAACGAGATTGTGAGTGTCAAGAGGGAATACGTAGTTTATGATCTGAAGACCCAAGTC
CCACCCCAGCAGCTGGTGCCCTGCTTCCAGGTGACGCTGAGGCTGAAGAACACGGCGCTCAAGTCCATCA
TCGCTCTCTTGGTGCCTGCAGAGGCACTGCTGTTGGCTGACGTGTGCGGGGGGTTGCTGCCCCTCCGGGC
CATTGAGCGCATAGGCTACAAGGTGACATTGCTGCTGAGTTACCTCGTCCTCCACTCCTCCCTGGTGCAG
GCCCTGCCCAGCTCCTCCTCCTGCAACCCACTGCTCATTTACTACTTCACCATCCTGCTGCTGCTGCTCT
TCCTCAGCACCATAGAGACTGTGCTGCTGGCTGGGCTGCTGGCCCGGGGCAACCTTGGGGCCAAGAGCGG
CCCCAGCCCAGCCCCGAGAGGGGAACAGCGAGAGCACGGCAACCCAGGGCCTCATCCTGCTGAAGAGCCC
TCCAGAGGAGTAAAGGGGTCACAGAGAAGCTGGCCTGAGACTGCTGACCGCATCTTCTTCCTCGTGTATG
TGGTTGGGGTGCTGTGCACCCAATTCGTCTTTGCAGGAATCTGGATGTGGGCAGCGTGCAAGTCTGACGC
AGCCCCTGGAGAGGCTGCACCCCATGGCAGGCGGCCTAGACTG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC212824 protein sequence
Red=Cloning site Green=Tags(s)

MALWSLLHLTFLGFSITLLLVHGQGFQGTAAIWPSLFNVNLSKKVQESIQIPNNGSAPLLVDVRVFVSNV
FNVDILRYTMSSMLLLRLSWLDTRLAWNTSAHPRHAITLPWESLWTPRLTILEALWVDWRDQSPQARVDQ
DGHVKLNLALTTETNCNFELLHFPRDHSNCSLSFYALSNTAMELEFQAHVVNEIVSVKREYVVYDLKTQV
PPQQLVPCFQVTLRLKNTALKSIIALLVPAEALLLADVCGGLLPLRAIERIGYKVTLLLSYLVLHSSLVQ
ALPSSSSCNPLLIYYFTILLLLLFLSTIETVLLAGLLARGNLGAKSGPSPAPRGEQREHGNPGPHPAEEP
SRGVKGSQRSWPETADRIFFLVYVVGVLCTQFVFAGIWMWAACKSDAAPGEAAPHGRRPRL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_180990
ORF Size 1233 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_180990.4
RefSeq Size 1473 bp
RefSeq ORF 1239 bp
Locus ID 353174
UniProt ID Q401N2
Cytogenetics 17q25.1
Protein Families Druggable Genome, Ion Channels: Other, Transmembrane
MW 45.7 kDa
Summary LGICZ1 is a zinc-activated ligand-gated ion channel that defines a new subgroup of the cysteine-loop superfamily of ligand-gated ion channels (Davies et al., 2003 PubMed 12381728).supplied by OMIM, Mar 2008
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Documents
Resources
"NM_180990" in other vectors (5)
SKU Description Size Price
RC212824L3 Lenti ORF clone of Human zinc activated ligand-gated ion channel (ZACN), Myc-DDK-tagged 10 ug
$757.00
RC212824L4 Lenti ORF clone of Human zinc activated ligand-gated ion channel (ZACN), mGFP tagged 10 ug
$757.00
RG212824 ZACN (tGFP-tagged) - Human zinc activated ligand-gated ion channel (ZACN) 10 ug
$489.00 $657.00
SC122050 ZACN (untagged)-Human zinc activated ligand-gated ion channel (ZACN) 10 ug
$457.00
SC327839 ZACN (untagged)-Human zinc activated ligand-gated ion channel (ZACN) 10 ug
$457.00

Plasmid Map for RC212824

Download sequence for

Cloning scheme for RC212824

Circular map for RC212824

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