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SHISA3 (NM_001080505) Human Tagged ORF Clone

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SKU
RC212334
SHISA3 (Myc-DDK-tagged)-Human shisa homolog 3 (Xenopus laevis) (SHISA3)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $300.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol SHISA3
Synonyms hShisa3
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC212334 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGGGCACTGCTGGCGCTTTGCCTTCTCCTTGGCTGTCTGCGCTGGGGCCCGGCGGGCGCCCAGCAGT
CCGGAGAGTACTGCCACGGCTGGGTGGACGTGCAGGGCAACTACCACGAGGGCTTCCAGTGCCCAGAGGA
CTTCGACACGCTGGACGCTACCATCTGCTGCGGCTCCTGCGCGCTCCGCTACTGTTGCGCCGCGGCCGAC
GCCAGGCTGGAGCAGGGCGGCTGCACCAACGACCGCCGCGAACTGGAGCACCCAGGCATCACTGCGCAGC
CTGTCTACGTCCCCTTTCTCATCGTCGGCTCCATCTTCATTGCGTTCATCATCCTGGGCTCTGTAGTGGC
TATTTATTGTTGCACCTGTTTGAGACCCAAGGAGCCCTCGCAGCAGCCAATCCGCTTCTCACTCCGCAGC
TATCAGACAGAGACCCTGCCCATGATCCTGACCTCCACCAGCCCCAGGGCACCCTCCCGGCAGTCCAGCA
CAGCCACGAGCTCCAGCTCCACAGGCGGCTCCATCCGCAGGTTCTCCTTTGCCAGGGCTGAGCCGGGCTG
CCTGGTGCCCTCACCGCCCCCGCCATACACCACCAGCCACTCAATCCACCTGGCTCAGCCATCTGGTTTC
CTGGTGTCACCCCAGTATTTCGCTTACCCCCTCCAGCAGGAGCCCCCACTGCCTGGGAAGAGCTGTCCAG
ACTTCAGTTCCAGT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC212334 protein sequence
Red=Cloning site Green=Tags(s)

MRALLALCLLLGCLRWGPAGAQQSGEYCHGWVDVQGNYHEGFQCPEDFDTLDATICCGSCALRYCCAAAD
ARLEQGGCTNDRRELEHPGITAQPVYVPFLIVGSIFIAFIILGSVVAIYCCTCLRPKEPSQQPIRFSLRS
YQTETLPMILTSTSPRAPSRQSSTATSSSSTGGSIRRFSFARAEPGCLVPSPPPPYTTSHSIHLAQPSGF
LVSPQYFAYPLQQEPPLPGKSCPDFSSS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001080505
ORF Size 714 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001080505.1, NP_001073974.1
RefSeq Size 1971 bp
RefSeq ORF 717 bp
Locus ID 152573
UniProt ID A0PJX4
Cytogenetics 4p13
Protein Families Transmembrane
MW 25.7 kDa
Summary This gene encodes a single-transmembrane protein which is one of nine members of a family of transmembrane adaptors that modulate both WNT and FGF signaling by blocking the maturation and transport of their receptors to the cell surface. Members of this family contain an N-terminal cysteine-rich domain with a distinct cysteine pattern, a single transmembrane domain, and a C-terminal proline-rich, low complexity region. The encoded protein acts as a tumor suppressor by accelerating beta-catenin degradation. provided by RefSeq, Jul 2017
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"NM_001080505" in other vectors (6)
SKU Description Size Price
RC212334L1 Lenti ORF clone of Human shisa homolog 3 (Xenopus laevis) (SHISA3), Myc-DDK-tagged 10 ug
$600.00
RC212334L2 Lenti ORF clone of Human shisa homolog 3 (Xenopus laevis) (SHISA3), mGFP tagged 10 ug
$600.00
RC212334L3 Lenti ORF clone of Human shisa homolog 3 (Xenopus laevis) (SHISA3), Myc-DDK-tagged 10 ug
$600.00
RC212334L4 Lenti ORF clone of Human shisa homolog 3 (Xenopus laevis) (SHISA3), mGFP tagged 10 ug
$600.00
RG212334 SHISA3 (tGFP-tagged) - Human shisa homolog 3 (Xenopus laevis) (SHISA3) 10 ug
$489.00 $500.00
SC315698 SHISA3 (untagged)-Human shisa homolog 3 (Xenopus laevis) (SHISA3) 10 ug
$330.00

Plasmid Map for RC212334

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC212334
Circular map for RC212334

file-alt Citations

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