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PPAPDC1A (PLPP4) (NM_001030059) Human Tagged ORF Clone

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SKU
RC212281
PPAPDC1A (Myc-DDK-tagged)-Human phosphatidic acid phosphatase type 2 domain containing 1A (PPAPDC1A)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $300.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol PPAPDC1A
Synonyms DPPL2; PPAPDC1; PPAPDC1A
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC212281 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCGGGAGCTGGCCATTGAGATCGGGGTGCGAGCCCTGCTCTTCGGAGTCTTCGTTTTTACAGAGTTTT
TGGATCCGTTCCAGAGAGTCATCCAGCCAGAAGAGATCTGGCTCTATAAAAATCCTTTGGTGCAATCAGA
TAACATACCTACCCGCCTCATGTTTGCAATTTCTTTCCTCACACCCCTGGCTGTTATTTGTGTGGTGAAA
ATTATCCGGCGAACAGACAAGACTGAAATTAAGGAAGCCTTCTTAGCGGTGTCCTTGGCTCTTGCTTTGA
ATGGAGTCTGCACAAACACTATTAAATTAATAGTGGGAAGACCTCGCCCCGATTTCTTTTACCGCTGCTT
TCCAGATGGAGTGATGAACTCGGAAATGCATTGCACAGGTGACCCCGATCTGGTGTCCGAGGGCCGCAAA
AGCTTCCCCAGCATCCATTCCTCCTTTGCCTTTTCGGGCCTTGGCTTCACGACGTTCTACTTGGCGGGCA
AGCTGCACTGCTTCACCGAGAGTGGGCGGGGAAAGAGCTGGCGGCTCTGTGCTGCCATCCTGCCCTTGTA
CTGCGCCATGATGATTGCCCTGTCCCGCATGTGCGACTACAAGCATCACTGGCAAGATTCCTTTGTGGGT
GGAGTCATCGGCCTCATTTTTGCATACATTTGCTACAGACAGCACTATCCTCCTCTGGCCAACACAGCTT
GCCATAAACCCTACGTTAGTCTGCGAGTCCCAGCCTCACTGAAGAAAGAGGAGAGGCCCACAGCTGACAG
CGCACCCAGCTTGCCTCTGGAGGGGATCACCGAAGGCCCGGTA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC212281 protein sequence
Red=Cloning site Green=Tags(s)

MRELAIEIGVRALLFGVFVFTEFLDPFQRVIQPEEIWLYKNPLVQSDNIPTRLMFAISFLTPLAVICVVK
IIRRTDKTEIKEAFLAVSLALALNGVCTNTIKLIVGRPRPDFFYRCFPDGVMNSEMHCTGDPDLVSEGRK
SFPSIHSSFAFSGLGFTTFYLAGKLHCFTESGRGKSWRLCAAILPLYCAMMIALSRMCDYKHHWQDSFVG
GVIGLIFAYICYRQHYPPLANTACHKPYVSLRVPASLKKEERPTADSAPSLPLEGITEGPV

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001030059
ORF Size 813 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001030059.3
RefSeq Size 1521 bp
RefSeq ORF 816 bp
Locus ID 196051
UniProt ID Q5VZY2
Cytogenetics 10q26.12
Protein Families Transmembrane
MW 30.4 kDa
Summary Displays magnesium-independent phosphatidate phosphatase activity in vitro. Catalyzes the conversion of phosphatidic acid to diacylglycerol.UniProtKB/Swiss-Prot Function
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"NM_001030059" in other vectors (4)
SKU Description Size Price
RC212281L3 Lenti ORF clone of Human phosphatidic acid phosphatase type 2 domain containing 1A (PPAPDC1A), Myc-DDK-tagged 10 ug
$600.00
RC212281L4 Lenti ORF clone of Human phosphatidic acid phosphatase type 2 domain containing 1A (PPAPDC1A), mGFP tagged 10 ug
$600.00
RG212281 PPAPDC1A (tGFP-tagged) - Human phosphatidic acid phosphatase type 2 domain containing 1A (PPAPDC1A) 10 ug
$489.00 $500.00
SC302504 PPAPDC1A (untagged)-Human phosphatidic acid phosphatase type 2 domain containing 1A (PPAPDC1A) 10 ug
$330.00

Plasmid Map for RC212281

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC212281
Circular map for RC212281

file-alt Citations

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