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PPAPDC1A (PLPP4) (NM_001030059) Human Untagged Clone

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SKU
SC302504
PPAPDC1A (untagged)-Human phosphatidic acid phosphatase type 2 domain containing 1A (PPAPDC1A)
  $330.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol PPAPDC1A
Synonyms DPPL2; PPAPDC1; PPAPDC1A
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>SC302504 representing NM_001030059.
Blue=Insert sequence Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGCGGGAGCTGGCCATTGAGATCGGGGTGCGAGCCCTGCTCTTCGGAGTCTTCGTTTTTACAGAGTTT
TTGGATCCGTTCCAGAGAGTCATCCAGCCAGAAGAGATCTGGCTCTATAAAAATCCTTTGGTGCAATCA
GATAACATACCTACCCGCCTCATGTTTGCAATTTCTTTCCTCACACCCCTGGCTGTTATTTGTGTGGTG
AAAATTATCCGGCGAACAGACAAGACTGAAATTAAGGAAGCCTTCTTAGCGGTGTCCTTGGCTCTTGCT
TTGAATGGAGTCTGCACAAACACTATTAAATTAATAGTGGGAAGACCTCGCCCCGATTTCTTTTACCGC
TGCTTTCCAGATGGAGTGATGAACTCGGAAATGCATTGCACAGGTGACCCCGATCTGGTGTCCGAGGGC
CGCAAAAGCTTCCCCAGCATCCATTCCTCCTTTGCCTTTTCGGGCCTTGGCTTCACGACGTTCTACTTG
GCGGGCAAGCTGCACTGCTTCACCGAGAGTGGGCGGGGAAAGAGCTGGCGGCTCTGTGCTGCCATCCTG
CCCTTGTACTGCGCCATGATGATTGCCCTGTCCCGCATGTGCGACTACAAGCATCACTGGCAAGATTCC
TTTGTGGGTGGAGTCATCGGCCTCATTTTTGCATACATTTGCTACAGACAGCACTATCCTCCTCTGGCC
AACACAGCTTGCCATAAACCCTACGTTAGTCTGCGAGTCCCAGCCTCACTGAAGAAAGAGGAGAGGCCC
ACAGCTGACAGCGCACCCAGCTTGCCTCTGGAGGGGATCACCGAAGGCCCGGTATGA
ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites SgfI-MluI |
ACCN NM_001030059
Insert Size 816 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001030059.2
RefSeq Size 1543 bp
RefSeq ORF 816 bp
Locus ID 196051
UniProt ID Q5VZY2
Cytogenetics 10q26.12
Protein Families Transmembrane
MW 30.4 kDa
Summary Displays magnesium-independent phosphatidate phosphatase activity in vitro. Catalyzes the conversion of phosphatidic acid to diacylglycerol.UniProtKB/Swiss-Prot Function
Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a).
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"NM_001030059" in other vectors (4)
SKU Description Size Price
RC212281 PPAPDC1A (Myc-DDK-tagged)-Human phosphatidic acid phosphatase type 2 domain containing 1A (PPAPDC1A) 10 ug
$300.00
RC212281L3 Lenti ORF clone of Human phosphatidic acid phosphatase type 2 domain containing 1A (PPAPDC1A), Myc-DDK-tagged 10 ug
$600.00
RC212281L4 Lenti ORF clone of Human phosphatidic acid phosphatase type 2 domain containing 1A (PPAPDC1A), mGFP tagged 10 ug
$600.00
RG212281 PPAPDC1A (tGFP-tagged) - Human phosphatidic acid phosphatase type 2 domain containing 1A (PPAPDC1A) 10 ug
$489.00 $500.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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