AP1B1 (NM_001127) Human Tagged ORF Clone

SKU RC211920

AP1B1 (Myc-DDK-tagged)-Human adaptor-related protein complex 1, beta 1 subunit (AP1B1), transcript variant 1

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: AP1B1
• Synonyms: ADTB1; AP105A; BAM22; CLAPB2; KIDAR
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC211920 representing NM_001127; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGACTGACTCAAAATATTTCACCACGACCAAGAAAGGGGAGATCTTCGAGCTGAAGGCAGAGCTCAACA; GTGACAAGAAGGAGAAGAAGAAGGAGGCAGTGAAGAAAGTGATTGCATCGATGACCGTGGGCAAAGATGT; CAGTGCCCTCTTCCCCGATGTGGTCAACTGCATGCAGACGGACAACCTGGAGCTGAAGAAGCTAGTATAC; CTCTACTTGATGAATTACGCCAAGAGTCAGCCTGACATGGCCATTATGGCCGTCAACACCTTTGTGAAGG; ACTGTGAGGACCCCAACCCCCTCATCCGAGCCCTGGCAGTGCGGACCATGGGCTGCATCCGCGTTGACAA; GATCACAGAGTACCTGTGCGAGCCACTCCGGAAGTGCCTGAAGGACGAGGATCCATATGTGCGCAAGACA; GCAGCTGTGTGCGTGGCCAAGCTCCACGACATCAACGCCCAGCTGGTGGAGGACCAGGGCTTCCTGGACA; CCCTTAAAGACCTCATCTCCGACTCTAACCCCATGGTGGTGGCCAATGCAGTGGCAGCGCTCTCAGAAAT; TGCCGAGTCTCACCCCAGCAGCAACCTGCTCGATCTGAACCCACAGTCCATCAACAAGCTGCTGACAGCC; CTCAATGAGTGCACCGAGTGGGGCCAGATCTTCATCCTGGACTGCCTCGCCAACTATATGCCCAAGGACG; ACCGCGAGGCCCAGAGCATCTGTGAGCGGGTCACCCCCAGGCTCTCCCATGCCAACTCCGCTGTGGTGCT; CTCTGCTGTGAAGGTGCTGATGAAGTTCATGGAGATGTTGTCTAAGGACTTGGACTACTACGGCACACTG; CTCAAGAAGCTGGCCCCACCCCTGGTCACACTGCTGTCAGCCGAGCCAGAGCTGCAGTATGTGGCCCTGC; GCAACATCAATCTCATCGTGCAGAAAAGGCCTGAGATCCTGAAGCATGAGATGAAGGTGTTCTTCGTGAA; GTACAACGACCCTATCTACGTGAAGCTGGAGAAGCTGGACATCATGATCCGCCTGGCCTCTCAGGCCAAC; ATCGCCCAGGTGTTGGCAGAGCTGAAAGAGTACGCAACAGAAGTGGATGTGGACTTTGTACGGAAGGCTG; TGCGTGCTATTGGCCGCTGCGCCATCAAGGTGGAGCAATCTGCGGAGCGCTGTGTGAGCACGCTGCTCGA; CCTCATCCAGACCAAGGTCAACTATGTGGTCCAGGAGGCCATCGTGGTCATCAAGGACATCTTCCGCAAG; TACCCCAACAAGTATGAGAGTGTGATTGCCACACTGTGTGAGAATCTGGACTCCCTGGATGAGCCTGAGG; CCCGGGCTGCCATGATCTGGATTGTGGGCGAGTACGCGGAACGGATCGACAACGCAGATGAGCTGCTGGA; GAGCTTCCTCGAGGGCTTCCATGACGAGAGCACACAGGTCCAGCTGCAGCTGCTGACAGCCATTGTGAAA; CTCTTTCTAAAGAAGCCAACAGAGACCCAGGAGCTGGTGCAGCAGGTCCTCAGTTTGGCCACTCAGGACT; CAGATAACCCAGACCTGCGGGACCGTGGCTACATCTACTGGCGCCTGCTGTCCACGGACCCGGTGGCAGC; CAAGGAGGTGGTGTTGGCTGAGAAGCCACTCATCTCTGAAGAGACGGACCTCATCGAGCCCACACTGTTA; GACGAGCTTATCTGCTACATCGGCACGCTGGCTTCCGTCTACCATAAGCCTCCCAGTGCCTTTGTGGAGG; GGGGCCGGGGCGTCGTGCACAAGAGCCTGCCACCTCGCACGGCCTCGAGTGAGAGCGCAGAGAGCCCTGA; GACAGCCCCTACTGGAGCACCTCCTGGGGAGCAGCCAGATGTCATCCCCGCCCAGGGCGACCTGCTGGGT; GACCTCCTCAACCTGGACCTCGGCCCCCCAGTGAGCGGCCCACCCCTGGCCACCTCCTCGGTGCAGATGG; GAGCTGTGGACCTTCTTGGCGGTGGCCTTGACAGCCTGATGGGGGATGAGCCTGAAGGGATTGGGGGCAC; CAACTTCGTGGCACCTCCAACAGCAGCAGTACCAGCCAATCTTGGAGCACCCATCGGCAGTGGCCTGAGT; GACCTCTTTGACCTGACCAGTGGCGTGGGCACCCTGTCAGGATCATATGTGGCCCCCAAAGCAGTCTGGC; TCCCAGCCATGAAGGCTAAGGGGCTGGAGATCTCAGGCACCTTCACCCGCCAGGTGGGCTCCATCTCCAT; GGACCTGCAGCTGACCAACAAGGCCTTGCAGGTCATGACCGACTTTGCCATCCAGTTCAACCGCAACAGC; TTTGGCCTGGCCCCCGCCGCCCCCCTCCAGGTCCACGCGCCACTCAGCCCCAACCAGACAGTGGAGATCT; CCCTGCCTCTCAGCACGGTGGGCTCGGTCATGAAGATGGAGCCTCTGAACAACCTCCAGGTGGCCGTGAA; GAACAACATCGATGTCTTCTACTTCAGCACCTTGTACCCACTGCACATCCTCTTTGTGGAGGACGGGAAG; ATGGACCGGCAGATGTTCCTGGCCACATGGAAGGATATTCCCAATGAGAATGAGGCCCAGTTCCAGATCA; GAGACTGCCCCCTCAATGCAGAGGCTGCGAGCAGCAAGCTGCAGAGCAGCAACATCTTCACTGTCGCCAA; GAGGAACGTGGAGGGCCAGGACATGCTCTACCAGTCCCTGAAGCTGACCAACGGCATCTGGGTGCTGGCG; GAGCTGCGGATCCAGCCGGGCAACCCCAGCTGCACGGACTTAGAGCTGTCCCTGAAGTGTCGAGCACCAG; AGGTGTCCCAGCACGTGTACCAGGCCTACGAGACCATCCTCAAGAAC; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC211920 representing NM_001127; Red=Cloning site Green=Tags(s); ; MTDSKYFTTTKKGEIFELKAELNSDKKEKKKEAVKKVIASMTVGKDVSALFPDVVNCMQTDNLELKKLVY; LYLMNYAKSQPDMAIMAVNTFVKDCEDPNPLIRALAVRTMGCIRVDKITEYLCEPLRKCLKDEDPYVRKT; AAVCVAKLHDINAQLVEDQGFLDTLKDLISDSNPMVVANAVAALSEIAESHPSSNLLDLNPQSINKLLTA; LNECTEWGQIFILDCLANYMPKDDREAQSICERVTPRLSHANSAVVLSAVKVLMKFMEMLSKDLDYYGTL; LKKLAPPLVTLLSAEPELQYVALRNINLIVQKRPEILKHEMKVFFVKYNDPIYVKLEKLDIMIRLASQAN; IAQVLAELKEYATEVDVDFVRKAVRAIGRCAIKVEQSAERCVSTLLDLIQTKVNYVVQEAIVVIKDIFRK; YPNKYESVIATLCENLDSLDEPEARAAMIWIVGEYAERIDNADELLESFLEGFHDESTQVQLQLLTAIVK; LFLKKPTETQELVQQVLSLATQDSDNPDLRDRGYIYWRLLSTDPVAAKEVVLAEKPLISEETDLIEPTLL; DELICYIGTLASVYHKPPSAFVEGGRGVVHKSLPPRTASSESAESPETAPTGAPPGEQPDVIPAQGDLLG; DLLNLDLGPPVSGPPLATSSVQMGAVDLLGGGLDSLMGDEPEGIGGTNFVAPPTAAVPANLGAPIGSGLS; DLFDLTSGVGTLSGSYVAPKAVWLPAMKAKGLEISGTFTRQVGSISMDLQLTNKALQVMTDFAIQFNRNS; FGLAPAAPLQVHAPLSPNQTVEISLPLSTVGSVMKMEPLNNLQVAVKNNIDVFYFSTLYPLHILFVEDGK; MDRQMFLATWKDIPNENEAQFQIRDCPLNAEAASSKLQSSNIFTVAKRNVEGQDMLYQSLKLTNGIWVLA; ELRIQPGNPSCTDLELSLKCRAPEVSQHVYQAYETILKN; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Chromatograms: Chromatograms ; Sequencher program is needed, download here
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001127
• ORF Size: 2847 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001127.4
• RefSeq Size: 4194 bp
• RefSeq ORF: 2850 bp
• Locus ID: 162
• UniProt ID: Q10567
• Cytogenetics: 22q12.2
• Domains: Adaptin_N, Alpha_adaptinC2
• Protein Pathways: Lysosome
• MW: 104.6 kDa
• Summary: Adaptor protein complex 1 is found at the cytoplasmic face of coated vesicles located at the Golgi complex, where it mediates both the recruitment of clathrin to the membrane and the recognition of sorting signals within the cytosolic tails of transmembrane receptors. This complex is a heterotetramer composed of two large, one medium, and one small adaptin subunit. The protein encoded by this gene serves as one of the large subunits of this complex and is a member of the adaptin protein family. This gene is a candidate meningioma gene. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2009]