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CCNO (NM_021147) Human Tagged ORF Clone

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SKU
RC211891
CCNO (Myc-DDK-tagged)-Human cyclin O (CCNO)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $457.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol CCNO
Synonyms CCNU; CILD29; UDG2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC211891 representing NM_021147
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGTGACCCCCTGTCCCACCAGCCCCTCGAGCCCCGCCGCCCGAGCGGGGAGGCGGGACAACGACCAGA
ACCTTCGCGCCCCGGTGAAGAAGAGCAGGCGTCCGCGCCTCCGGAGGAAGCAGCCGCTGCATCCCCTGAA
CCCGTGCCCGCTCCCGGGAGACTCCGGCATTTGCGACCTGTTCGAGTCCCCCAGCTCCGGCTCAGACGGC
GCAGAGAGCCCCTCTGCGGCGCGGGGTGGTAGCCCCCTGCCCGGCCCGGCCCAGCCCGTGGCGCAGCTAG
ATCTACAGACCTTCCGCGACTACGGCCAGAGCTGCTACGCCTTCCGCAAGGCGCAGGAGAGCCACTTCCA
CCCGCGGGAGGCGCTGGCACGGCAGCCACAAGTGACGGCGGAATCCCGCTGTAAGCTGCTCAGCTGGCTG
ATCCCGGTGCACCGCCAATTCGGCCTCTCCTTCGAGTCGCTGTGCCTGACGGTGAACACTCTGGACCGCT
TCCTCACCACCACGCCGGTGGCTGCAGACTGCTTCCAGCTGCTTGGGGTCACCTCCTTGCTCATCGCTTG
CAAACAGGTGGAGGTGCACCCGCCGCGCGTGAAGCAGCTTCTGGCCCTCTGCTGCGGCGCCTTCTCCCGG
CAGCAGCTCTGCAACCTCGAGTGCATCGTGCTGCACAAGCTGCACTTCACCCTGGGTGCGCCCACCATTA
GCTTCTTCCTGGAGCATTTCACGCACGCTCGCGTGGAGGCGGGGCAGGCTGAGGCCTCCGAAGCTCTGGA
AGCGCAAGCCCTGGCGCGGGGGGTGGCAGAGCTGAGTCTGGCCGACTATGCCTTCACCAGCTACTCCCCT
TCCCTCCTGGCGATCTGCTGCCTGGCGCTGGCGGACCGCATGCTGCGGGTCTCGCGGCCCGTGGACTTGC
GACTGGGAGACCACCCGGAGGCGGCGCTGGAGGACTGTATGGGCAAGTTGCAGCTGCTGGTGGCCATAAA
CAGTACTTCCTTGACTCACATGCTGCCCGTTCAGATCTGCGAGAAGTGCAGCCTGCCCCCGAGCTCGAAA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC211891 representing NM_021147
Red=Cloning site Green=Tags(s)

MVTPCPTSPSSPAARAGRRDNDQNLRAPVKKSRRPRLRRKQPLHPLNPCPLPGDSGICDLFESPSSGSDG
AESPSAARGGSPLPGPAQPVAQLDLQTFRDYGQSCYAFRKAQESHFHPREALARQPQVTAESRCKLLSWL
IPVHRQFGLSFESLCLTVNTLDRFLTTTPVAADCFQLLGVTSLLIACKQVEVHPPRVKQLLALCCGAFSR
QQLCNLECIVLHKLHFTLGAPTISFFLEHFTHARVEAGQAEASEALEAQALARGVAELSLADYAFTSYSP
SLLAICCLALADRMLRVSRPVDLRLGDHPEAALEDCMGKLQLLVAINSTSLTHMLPVQICEKCSLPPSSK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_021147
ORF Size 1050 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_021147.5
RefSeq Size 1448 bp
RefSeq ORF 1053 bp
Locus ID 10309
UniProt ID P22674
Cytogenetics 5q11.2
MW 37.9 kDa
Summary This gene encodes a member of the cyclin protein family, and the encoded protein is involved in regulation of the cell cycle. Disruption of this gene is associated with primary ciliary dyskinesia-19. Alternative splicing results in multiple transcript variants. This gene, which has a previous symbol of UNG2, was erroneously identified as a uracil DNA glycosylase in PubMed ID: 2001396. A later publication, PubMed ID: 8419333, identified this gene's product as a cyclin protein family member. The UNG2 symbol is also used as a specific protein isoform name for the UNG gene (GeneID 7374), so confusion exists in the scientific literature and in some databases for these two genes. provided by RefSeq, Jul 2014
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Documents
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"NM_021147" in other vectors (6)
SKU Description Size Price
RC211891L1 Lenti ORF clone of Human cyclin O (CCNO), Myc-DDK-tagged 10 ug
$757.00
RC211891L2 Lenti ORF clone of Human cyclin O (CCNO), mGFP tagged 10 ug
$757.00
RC211891L3 Lenti ORF clone of Human cyclin O (CCNO), Myc-DDK-tagged 10 ug
$757.00
RC211891L4 Lenti ORF clone of Human cyclin O (CCNO), mGFP tagged 10 ug
$757.00
RG211891 CCNO (tGFP-tagged) - Human cyclin O (CCNO) 10 ug
$657.00
SC304913 CCNO (untagged)-Human cyclin O (CCNO) 10 ug
$503.00

Plasmid Map for RC211891

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC211891
Circular map for RC211891

file-alt Citations

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