DYNLT2 (NM_174910) Human Tagged ORF Clone

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SKU
RC211870
TCTE3 (Myc-DDK-tagged)-Human t-complex-associated-testis-expressed 3 (TCTE3)
  $300.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol DYNLT2
Synonyms TCTE3; TCTEX1D3; TCTEX2; Tctex4
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC211870 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGAAGCGAGGCCGAGGCGTGAAGTCGAGCCCCATCCAGACCCCGAACCAGACCCCTCAGCAGGCTC
CGGTGACGCCTAGGAAAGAAAGGAGGCCTAGCATGTTCGAGAAGGAGGCATATACACAGATTTTAAGAGA
AAGACTGAGAGAGTCAATTCACAATGTTCAGTATGTGGAGCCTCCTTTTGATGACTCAATTGCTGATATA
GGTAAAGAATGGAAGAGTGCCCTGGCAAAATTAAAGTTTGCTAATTCATATAGAATGGAGCCATTGAAGA
AATTCCAAGCTCATTCAGTAGAAACTAAAGTCCAGCAGATACTAACAGAAAGTCTTAAAGATGTCAAATA
TGATGATAAAGTATTCTCTCACTTGTCACTTGAATTGGCAGACCGCATACTGTTAGCAGTCAAAGAATTT
GGGTACCACCGTTATAAGTTCATTATAAAAGTATTATTTATTCAAAAGACTGGCCAAGCAATAAATATTG
CCAGCAGATGGATCTGGGACATTGCATGGGACAGCTGGGTCGCAGCTAAACACGAAGCAGAATCCTACGT
GGCACTGGTCTTGGTGTTTGCCCTTTATTATGAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC211870 protein sequence
Red=Cloning site Green=Tags(s)

MEKRGRGVKSSPIQTPNQTPQQAPVTPRKERRPSMFEKEAYTQILRERLRESIHNVQYVEPPFDDSIADI
GKEWKSALAKLKFANSYRMEPLKKFQAHSVETKVQQILTESLKDVKYDDKVFSHLSLELADRILLAVKEF
GYHRYKFIIKVLFIQKTGQAINIASRWIWDIAWDSWVAAKHEAESYVALVLVFALYYE

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_174910
ORF Size 594 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_174910.3
RefSeq Size 747 bp
RefSeq ORF 597 bp
Locus ID 6991
UniProt ID Q8IZS6
Cytogenetics 6q27
MW 23.2 kDa
Summary May be an accessory component of axonemal dynein and cytoplasmic dynein 1. Candidate for involvement in male sterility (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_174910" in other vectors (4)
SKU Description Size Price
RC211870L3 Lenti ORF clone of Human t-complex-associated-testis-expressed 3 (TCTE3), Myc-DDK-tagged 10 ug
$600.00
RC211870L4 Lenti ORF clone of Human t-complex-associated-testis-expressed 3 (TCTE3), mGFP tagged 10 ug
$600.00
RG211870 TCTE3 (tGFP-tagged) - Human t-complex-associated-testis-expressed 3 (TCTE3) 10 ug
$500.00
SC306954 TCTE3 (untagged)-Human t-complex-associated-testis-expressed 3 (TCTE3) 10 ug
$330.00

Plasmid Map for RC211870

Download sequence for

Cloning scheme for RC211870

Circular map for RC211870

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