RNF121 (NM_018320) Human Tagged ORF Clone
$450.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | RNF121 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_018320 |
| ORF Size | 981 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_018320.5 |
| RefSeq Size | 2306 bp |
| RefSeq ORF | 984 bp |
| Locus ID | 55298 |
| UniProt ID | Q9H920 |
| Cytogenetics | 11q13.4 |
| Domains | RING |
| Protein Families | Druggable Genome, Transmembrane |
| MW | 37.7 kDa |
| Summary | The protein encoded by this gene contains a RING finger, a motif present in a variety of functionally distinct proteins and known to be involved in protein-protein and protein-DNA interactions. Several alternatively spliced transcript variants have been noted for this gene, however, not all are likely to encode viable protein products. provided by RefSeq, Sep 2008 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_018320" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC211747L1 | Lenti ORF clone of Human ring finger protein 121 (RNF121), transcript variant 1, Myc-DDK-tagged | 10 ug |
$750.00
|
|
| RC211747L2 | Lenti ORF clone of Human ring finger protein 121 (RNF121), transcript variant 1, mGFP tagged | 10 ug |
$750.00
|
|
| RC211747L3 | Lenti ORF clone of Human ring finger protein 121 (RNF121), transcript variant 1, Myc-DDK-tagged | 10 ug |
$750.00
|
|
| RC211747L4 | Lenti ORF clone of Human ring finger protein 121 (RNF121), transcript variant 1, mGFP tagged | 10 ug |
$750.00
|
|
| RG211747 | RNF121 (tGFP-tagged) - Human ring finger protein 121 (RNF121), transcript variant 1 | 10 ug |
$650.00
|
|
| SC310532 | RNF121 (untagged)-Human ring finger protein 121 (RNF121), transcript variant 1 | 10 ug |
$450.00
|
|
Citations
Golgi-localized Ring Finger Protein 121 is necessary for MYCN-driven neuroblastoma tumorigenesis
Cheung, Mittra, Murray et al
Commun Biol (2024) 7 (1), 1322 DOI: 10.1038/s42003-024-06899-8
Cheung, Mittra, Murray et al
Commun Biol (2024) 7 (1), 1322 DOI: 10.1038/s42003-024-06899-8
Cited by 4
AI Summary: OriGene contributed pCMV6-Empty Vector and pCMV6-hRNF121 wild type (RC211747) expression plasmids, which were used to study the role of RNF121 in MYCN-driven neuroblastoma tumorigenesis.
Snippet: Expression plasmids of pCMV6-Empty Vector and pCMV6-hRNF121 wild type (WT) with Myc-DDK (FLAG) at c-terminus were purchased from Origene (Origene RC211747). pCMV6-hRNF121(M158R), pCMV6-hRNF121-M1, pCMV6-hRNF121-M2, pCMV6-hRNF121-M3 and pCMV6-hRNF121-M4 with Myc-DDK(FLAG) at c-terminus were subcloned and mutagenesis were performed by Genescript based on RC211747 RNF121 sequence (Genescript)
Showing 1-1 of 1 papers.
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