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IL36RN (NM_012275) Human Tagged ORF Clone

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SKU
RC211691
IL36RN (Myc-DDK-tagged)-Human interleukin 1 family, member 5 (delta) (IL1F5), transcript variant 1
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $150.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol IL36RN
Synonyms FIL1; FIL1(DELTA); FIL1D; IL-36Ra; IL1F5; IL1HY1; IL1L1; IL1RP3; IL36RA; PSORP; PSORS14
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC211691 representing NM_012275
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGTCCTGAGTGGGGCGCTGTGCTTCCGAATGAAGGACTCGGCATTGAAGGTGCTTTATCTGCATAATA
ACCAGCTTCTAGCTGGAGGGCTGCATGCAGGGAAGGTCATTAAAGGTGAAGAGATCAGCGTGGTCCCCAA
TCGGTGGCTGGATGCCAGCCTGTCCCCCGTCATCCTGGGTGTCCAGGGTGGAAGCCAGTGCCTGTCATGT
GGGGTGGGGCAGGAGCCGACTCTAACACTAGAGCCAGTGAACATCATGGAGCTCTATCTTGGTGCCAAGG
AATCCAAGAGCTTCACCTTCTACCGGCGGGACATGGGGCTCACCTCCAGCTTCGAGTCGGCTGCCTACCC
GGGCTGGTTCCTGTGCACGGTGCCTGAAGCCGATCAGCCTGTCAGACTCACCCAGCTTCCCGAGAATGGT
GGCTGGAATGCCCCCATCACAGACTTCTACTTCCAGCAGTGTGAC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC211691 representing NM_012275
Red=Cloning site Green=Tags(s)

MVLSGALCFRMKDSALKVLYLHNNQLLAGGLHAGKVIKGEEISVVPNRWLDASLSPVILGVQGGSQCLSC
GVGQEPTLTLEPVNIMELYLGAKESKSFTFYRRDMGLTSSFESAAYPGWFLCTVPEADQPVRLTQLPENG
GWNAPITDFYFQQCD

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_012275
ORF Size 465 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_012275.3
RefSeq Size 2717 bp
RefSeq ORF 468 bp
Locus ID 26525
UniProt ID Q9UBH0
Cytogenetics 2q14.1
Domains IL1
Protein Families Druggable Genome, Secreted Protein
MW 16.8 kDa
Summary The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine was shown to specifically inhibit the activation of NF-kappaB induced by interleukin 1 family, member 6 (IL1F6). This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. Two alternatively spliced transcript variants encoding the same protein have been reported. provided by RefSeq, Jul 2008
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"NM_012275" in other vectors (6)
SKU Description Size Price
RC211691L1 Lenti ORF clone of Human interleukin 1 family, member 5 (delta) (IL1F5), transcript variant 1, Myc-DDK-tagged 10 ug
$450.00
RC211691L2 Lenti ORF clone of Human interleukin 1 family, member 5 (delta) (IL1F5), transcript variant 1, mGFP tagged 10 ug
$450.00
RC211691L3 Lenti ORF clone of Human interleukin 1 family, member 5 (delta) (IL1F5), transcript variant 1, Myc-DDK-tagged 10 ug
$450.00
RC211691L4 Lenti ORF clone of Human interleukin 1 family, member 5 (delta) (IL1F5), transcript variant 1, mGFP tagged 10 ug
$450.00
RG211691 IL36RN (tGFP-tagged) - Human interleukin 1 family, member 5 (delta) (IL1F5), transcript variant 1 10 ug
$489.00
SC115434 IL36RN (untagged)-Human interleukin 1 family, member 5 (delta) (IL1F5), transcript variant 1 10 ug
$150.00

Plasmid Map for RC211691

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC211691
Circular map for RC211691

file-alt Citations

Investigating Mechanisms of Immune Function and New Therapeutic Targets in Rare Autoimmune Diseases
Hecker
Thesis (2024)
Snippet: Plasmid Company Catalog number IL36RN (NM_173170) Human Tagged ORF Clone pCMV entry Origene, Rockville, USA RC211691 pCMV6-Entry IL36RN S113L Plasmid Addgene, Watertown, USA 58321 pET-(IL36RA) VectorBuilder, Chicago, USA VB220506-1124dka (Vector ID)
Showing 1-1 of 1 papers.
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