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CACNB2 (NM_201571) Human Tagged ORF Clone

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SKU
RC211669
CACNB2 (Myc-DDK-tagged)-Human calcium channel, voltage-dependent, beta 2 subunit (CACNB2), transcript variant 6
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $588.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol CACNB2
Synonyms CAB2; CACNLB2; CAVB2; MYSB
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC211669 representing NM_201571
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAATCAGGGGAGTGGACTGGACCTGCTGAAGATCTCATATGGAAAAGGAGCCAGAAGGAAAAACAGAT
TTAAAGGATCTGATGGAAGCACGTCATCTGATACTACCTCAAATAGTTTTGTTCGCCAGGGTTCGGCAGA
CTCCTACACTAGCCGTCCATCCGATTCCGATGTATCTCTGGAGGAGGACCGGGAGGCAGTGCGCAGAGAA
GCGGAGCGGCAGGCCCAGGCACAGTTGGAAAAAGCAAAGACAAAGCCCGTTGCATTTGCGGTTCGGACAA
ATGTCAGCTACAGTGCGGCCCATGAAGATGATGTTCCAGTGCCTGGCATGGCCATCTCATTCGAAGCAAA
AGATTTTCTGCATGTTAAGGAAAAATTTAACAATGACTGGTGGATAGGGCGATTGGTAAAAGAAGGCTGT
GAAATCGGATTCATTCCAAGCCCAGTCAAACTAGAAAACATGAGGCTGCAGCATGAACAGAGAGCCAAGC
AAGGGAAATTCTACTCCAGTAAATCAGGAGGAAATTCATCATCCAGTTTGGGTGACATAGTACCTAGTTC
CAGAAAATCAACACCTCCATCATCTGCTATAGACATAGATGCTACTGGCTTAGATGCAGAAGAAAATGAT
ATTCCAGCAAACCACCGCTCCCCTAAACCCAGTGCAAACAGTGTAACGTCACCCCACTCCAAAGAGAAAA
GAATGCCCTTCTTTAAGAAGACAGAGCACACTCCTCCGTATGATGTGGTACCTTCCATGCGACCAGTGGT
CCTAGTGGGCCCTTCTCTGAAGGGCTACGAGGTCACAGATATGATGCAAAAAGCGCTGTTTGATTTTTTA
AAACACAGATTTGAAGGGCGGATATCCATCACAAGGGTCACCGCTGACATCTCGCTTGCCAAACGCTCGG
TATTAAACAATCCCAGTAAGCACGCAATAATAGAAAGATCCAACACAAGGTCAAGCTTAGCGGAAGTTCA
GAGTGAAATCGAAAGGATTTTTGAACTTGCAAGAACATTGCAGTTGGTGGTCCTTGACGCGGATACAATT
AATCATCCAGCTCAACTCAGTAAAACCTCCTTGGCCCCTATTATAGTATATGTAAAGATTTCTTCTCCTA
AGGTTTTACAAAGGTTAATAAAATCTCGAGGGAAATCTCAAGCTAAACACCTCAACGTCCAGATGGTAGC
AGCTGATAAACTGGCTCAGTGTCCTCCAGAGCTGTTCGATGTGATCTTGGATGAGAACCAGCTTGAGGAT
GCCTGTGAGCACCTTGCCGACTATCTGGAGGCCTACTGGAAGGCCACCCATCCTCCCAGCAGTAGCCTCC
CCAACCCTCTCCTTAGCCGTACATTAGCCACTTCAAGTCTGCCTCTTAGCCCCACCCTAGCCTCTAATTC
ACAGGGTTCTCAAGGTGATCAGAGGACTGATCGCTCCGCTCCTATCCGTTCTGCTTCCCAAGCTGAAGAA
GAACCTAGTGTGGAACCAGTCAAGAAATCCCAGCACCGCTCTTCCTCCTCAGCCCCACACCACAACCATC
GCAGTGGGACAAGTCGCGGCCTCTCCAGGCAAGAGACATTTGACTCGGAAACCCAGGAGAGTCGAGACTC
TGCCTACGTAGAGCCAAAGGAAGATTATTCCCATGACCACGTGGACCACTATGCCTCACACCGTGACCAC
AACCACAGAGACGAGACCCACGGGAGCAGTGACCACAGACACAGGGAGTCCCGGCACCGTTCCCGGGACG
TGGATCGAGAGCAGGACCACAACGAGTGCAACAAGCAGCGCAGCCGTCATAAATCCAAGGATCGCTACTG
TGAAAAGGATGGAGAAGTGATATCAAAAAAACGGAATGAGGCTGGGGAGTGGAACAGGGATGTTTACATC
CGCCAA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC211669 representing NM_201571
Red=Cloning site Green=Tags(s)

MNQGSGLDLLKISYGKGARRKNRFKGSDGSTSSDTTSNSFVRQGSADSYTSRPSDSDVSLEEDREAVRRE
AERQAQAQLEKAKTKPVAFAVRTNVSYSAAHEDDVPVPGMAISFEAKDFLHVKEKFNNDWWIGRLVKEGC
EIGFIPSPVKLENMRLQHEQRAKQGKFYSSKSGGNSSSSLGDIVPSSRKSTPPSSAIDIDATGLDAEEND
IPANHRSPKPSANSVTSPHSKEKRMPFFKKTEHTPPYDVVPSMRPVVLVGPSLKGYEVTDMMQKALFDFL
KHRFEGRISITRVTADISLAKRSVLNNPSKHAIIERSNTRSSLAEVQSEIERIFELARTLQLVVLDADTI
NHPAQLSKTSLAPIIVYVKISSPKVLQRLIKSRGKSQAKHLNVQMVAADKLAQCPPELFDVILDENQLED
ACEHLADYLEAYWKATHPPSSSLPNPLLSRTLATSSLPLSPTLASNSQGSQGDQRTDRSAPIRSASQAEE
EPSVEPVKKSQHRSSSSAPHHNHRSGTSRGLSRQETFDSETQESRDSAYVEPKEDYSHDHVDHYASHRDH
NHRDETHGSSDHRHRESRHRSRDVDREQDHNECNKQRSRHKSKDRYCEKDGEVISKKRNEAGEWNRDVYI
RQ

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_201571
ORF Size 1896 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_201571.4
RefSeq Size 3301 bp
RefSeq ORF 1899 bp
Locus ID 783
UniProt ID Q08289
Cytogenetics 10p12.33-p12.31
Protein Families Druggable Genome, Ion Channels: Other
Protein Pathways Arrhythmogenic right ventricular cardiomyopathy (ARVC), Cardiac muscle contraction, Dilated cardiomyopathy, Hypertrophic cardiomyopathy (HCM), MAPK signaling pathway
MW 70.7 kDa
Summary This gene encodes a subunit of a voltage-dependent calcium channel protein that is a member of the voltage-gated calcium channel superfamily. The gene product was originally identified as an antigen target in Lambert-Eaton myasthenic syndrome, an autoimmune disorder. Mutations in this gene are associated with Brugada syndrome. Alternatively spliced variants encoding different isoforms have been described. provided by RefSeq, Feb 2013
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"NM_201571" in other vectors (4)
SKU Description Size Price
RC211669L3 Lenti-ORF clone of CACNB2 (Myc-DDK-tagged)-Human calcium channel, voltage-dependent, beta 2 subunit (CACNB2), transcript variant 6 10 ug
$888.00
RC211669L4 Lenti-ORF clone of CACNB2 (mGFP-tagged)-Human calcium channel, voltage-dependent, beta 2 subunit (CACNB2), transcript variant 6 10 ug
$888.00
RG211669 CACNB2 (tGFP-tagged) - Human calcium channel, voltage-dependent, beta 2 subunit (CACNB2), transcript variant 6 10 ug
$788.00
SC314275 CACNB2 (untagged)-Human calcium channel, voltage-dependent, beta 2 subunit (CACNB2), transcript variant 6 10 ug
$648.00

Plasmid Map for RC211669

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC211669
Circular map for RC211669

file-alt Citations

CACNB2 is associated with aberrant RAS-MAPK signaling in hypertensive Dahl Salt-Sensitive rats
Durairaj Pandian, Giovannucci, Vazquez et al
Biochem. Biophys. Res. Commun. (2019) 513 (3), 760-765 DOI: 10.1016/j.bbrc.2019.03.215
AI Summary: OriGene’s pCMV6-CACNB2 construct (OriGene, #RC211669) was used to transiently overexpress CACNB2 in HEK293 cells, enabling the study of its effects on RAS-MAPK pathway activation in the context of hypertension.
Snippet: and Penicillin-Streptomycin (1X) in a humidified incubator with 5% CO 2 . In brief, 3 x 10 5 cells were seeded per well in six well culture plates and after 60-70% confluent, cells were transiently transfected with 2 μg of pCMV6-CACNB2 construct (Origene, # RC211669
Showing 1-1 of 1 papers.
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