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KSR2 (NM_173598) Human Tagged ORF Clone

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SKU
RC211665
KSR2 (Myc-DDK-tagged)-Human kinase suppressor of ras 2 (KSR2)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
  $843.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol KSR2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC211665 representing NM_173598
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGATAGACTTGAGCATCTCCAACCTGGAAGGGCTTAGGACCAAATGTGCTACCTCCAACGACCTCACAC
AAAAAGAAATCCGGACCCTGGAGAGCAAGCTGGTGAAGTACTTCAGCCGGCAGCTGTCCTGCAAAAAGAA
GGTAGCCTTGCAGGAGCGCAACGCGGAGCTGGACGGCTTCCCCCAGCTACGGCACTGGTTCCGAATCGTC
GATGTGCGCAAGGAGGTCCTGGAGGAAATCTCCCCCGGCCAGCTGAGCCTGGAGGACCTCTTGGAGATGA
CGGATGAACAGGTGTGCGAGACTGTGGAGAAATACGGAGCCAACCGGGAGGAGTGTGCCCGCCTCAACGC
CTCCCTCTCCTGCCTCAGGAATGTCCACATGTCAGGAGGCAACCTTTCCAAACAAGACTGGACCATCCAG
TGGCCCACGACAGAGACGGGGAAGGAGAACAATCCCGTGTGCCCCCCGGAGCCCACCCCGTGGATCCGCA
CCCATCTCTCCCAGAGCCCCAGGGTCCCGTCCAAGTGCGTCCAGCACTATTGTCACACCAGCCCCACTCC
CGGGGCCCCTGTGTACACCCACGTGGACAGGCTTACCGTGGACGCCTACCCGGGCTTGTGCCCGCCCCCG
CCACTGGAGTCGGGCCACCGTTCCCTGCCCCCATCGCCCCGGCAGCGGCACGCGGTCCGCACCCCGCCGC
GCACCCCCAACATCGTCACCACCGTGACCCCGCCGGGCACGCCGCCCATGAGGAAGAAGAACAAGCTGAA
GCCCCCGGGGACCCCACCGCCCTCCTCCCGAAAACTGATACACTTGATCCCGGGATTCACCGCGCTGCAT
CGGAGCAAATCCCACGAGTTCCAGCTGGGGCACCGCGTGGACGAGGCCCACACGCCCAAAGCCAAGAAGA
AGAGCAAACCCTTGAACCTCAAGATCCACAGCAGCGTAGGCAGCTGCGAGAACATCCCCTCTCAGCAGCG
CTCCCCGCTGCTGTCCGAGCGCTCCCTCCGCTCCTTCTTTGTGGGACACGCACCTTTCCTGCCTTCCACC
CCTCCTGTTCACACTGAGGCCAACTTCTCTGCAAACACACTGTCAGTGCCACGCTGGTCCCCGCAGATCC
CTCGCAGAGATCTCGGCAACTCCATCAAGCACAGGTTTTCCACCAAGTACTGGATGTCTCAGACGTGCAC
AGTCTGTGGGAAAGGGATGCTTTTTGGCCTCAAGTGTAAAAACTGCAAGTTAAAGTGCCACAACAAATGC
ACCAAAGAAGCCCCACCCTGTCATCTTCTGATCATCCACCGAGGAGATCCAGCAAGGTTAGTCCGGACAG
AGTCCGTTCCGTGTGACATCAACAACCCTCTACGGAAGCCACCTCGCTATTCAGACCTGCACATCAGTCA
GACGCTCCCCAAAACCAACAAAATCAACAAGGACCACATCCCTGTCCCTTACCAGCCAGACTCCAGCAGC
AACCCCTCCTCCACGACGTCCTCCACGCCCTCCTCGCCAGCACCCCCCCTCCCTCCTAGTGCCACGCCGC
CTTCTCCCCTACACCCTTCCCCACAGTGCACACGGCAGCAGAAGAACTTCAACCTGCCAGCATCCCACTA
CTACAAATACAAGCAGCAGTTCATCTTCCCAGATGTGGTGCCGGTGCCGGAGACGCCGACCCGGGCGCCC
CAGGTCATCCTGCATCCGGTGACCTCGAATCCAATCTTGGAAGGAAATCCATTACTTCAAATTGAAGTGG
AGCCAACGTCGGAGAATGAAGAGGTCCATGATGAGGCCGAAGAGTCAGAGGATGACTTCGAGGAGATGAA
CCTGTCCCTCCTCTCGGCCCGGAGCTTCCCACGCAAGGCCAGCCAGACCAGCATCTTCCTTCAGGAGTGG
GACATCCCCTTTGAGCAGCTGGAGATCGGCGAGCTCATTGGAAAGGGCCGCTTTGGGCAAGTGTACCACG
GCCGCTGGCATGGCGAGGTGGCCATCCGGCTGATTGACATTGAGAGGGACAACGAGGACCAGCTCAAGGC
CTTCAAGCGGGAGGTGATGGCCTACAGGCAGACACGGCATGAGAACGTGGTGCTTTTCATGGGTGCCTGC
ATGAGCCCGCCTCACCTGGCCATCATCACCAGCCTCTGTAAGGGACGGACGCTCTATTCCGTTGTGAGGG
ATGCCAAAATCGTTTTGGATGTCAACAAAACCAGGCAGATTGCTCAAGAAATTGTGAAGGGCATGGGCTA
CCTCCACGCCAAGGGAATCCTACACAAGGACCTCAAGTCAAAGAACGTCTTCTATGACAACGGCAAAGTG
GTCATCACGGACTTTGGACTCTTCAGCATTTCTGGGGTGCTGCAGGCTGGCAGGCGGGAGGACAAACTGC
GCATCCAGAATGGCTGGCTATGCCACCTGGCACCAGAGATCATCCGCCAGCTGTCCCCCGACACAGAGGA
GGATAAGCTCCCCTTCTCCAAGCACTCTGACGTCTTTGCCCTTGGCACAATCTGGTATGAACTCCACGCC
AGGGAATGGCCTTTCAAGACCCAACCAGCAGAGGCAATAATCTGGCAAATGGGCACAGGCATGAAACCCA
ACCTCAGCCAGATTGGCATGGGAAAAGAAATCTCGGACATTCTTCTCTTCTGCTGGGCCTTTGAACAAGA
AGAGAGACCTACCTTCACCAAGCTCATGGACATGCTGGAGAAACTGCCAAAGCGAAACCGTCGCCTGTCT
CACCCTGGACATTTCTGGAAGTCTGCAGAGCTG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC211665 representing NM_173598
Red=Cloning site Green=Tags(s)

MIDLSISNLEGLRTKCATSNDLTQKEIRTLESKLVKYFSRQLSCKKKVALQERNAELDGFPQLRHWFRIV
DVRKEVLEEISPGQLSLEDLLEMTDEQVCETVEKYGANREECARLNASLSCLRNVHMSGGNLSKQDWTIQ
WPTTETGKENNPVCPPEPTPWIRTHLSQSPRVPSKCVQHYCHTSPTPGAPVYTHVDRLTVDAYPGLCPPP
PLESGHRSLPPSPRQRHAVRTPPRTPNIVTTVTPPGTPPMRKKNKLKPPGTPPPSSRKLIHLIPGFTALH
RSKSHEFQLGHRVDEAHTPKAKKKSKPLNLKIHSSVGSCENIPSQQRSPLLSERSLRSFFVGHAPFLPST
PPVHTEANFSANTLSVPRWSPQIPRRDLGNSIKHRFSTKYWMSQTCTVCGKGMLFGLKCKNCKLKCHNKC
TKEAPPCHLLIIHRGDPARLVRTESVPCDINNPLRKPPRYSDLHISQTLPKTNKINKDHIPVPYQPDSSS
NPSSTTSSTPSSPAPPLPPSATPPSPLHPSPQCTRQQKNFNLPASHYYKYKQQFIFPDVVPVPETPTRAP
QVILHPVTSNPILEGNPLLQIEVEPTSENEEVHDEAEESEDDFEEMNLSLLSARSFPRKASQTSIFLQEW
DIPFEQLEIGELIGKGRFGQVYHGRWHGEVAIRLIDIERDNEDQLKAFKREVMAYRQTRHENVVLFMGAC
MSPPHLAIITSLCKGRTLYSVVRDAKIVLDVNKTRQIAQEIVKGMGYLHAKGILHKDLKSKNVFYDNGKV
VITDFGLFSISGVLQAGRREDKLRIQNGWLCHLAPEIIRQLSPDTEEDKLPFSKHSDVFALGTIWYELHA
REWPFKTQPAEAIIWQMGTGMKPNLSQIGMGKEISDILLFCWAFEQEERPTFTKLMDMLEKLPKRNRRLS
HPGHFWKSAEL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_173598
ORF Size 2763 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_173598.4, NP_775869.3
RefSeq Size 17008 bp
RefSeq ORF 2853 bp
Locus ID 283455
UniProt ID Q6VAB6, E9PB13
Cytogenetics 12q24.22-q24.23
Protein Families Druggable Genome, Protein Kinase
MW 104.1 kDa
Summary Location-regulated scaffold connecting MEK to RAF. Has very low protein kinase activity and can phosphorylate MAP2K1 at several Ser and Thr residues with very low efficiency (in vitro). Interaction with BRAF enhances KSR2-mediated phosphorylation of MAP2K1 (in vitro). Blocks MAP3K8 kinase activity and MAP3K8-mediated signaling. Acts as a negative regulator of MAP3K3-mediated activation of ERK, JNK and NF-kappa-B pathways, inhibiting MAP3K3-mediated interleukin-8 production.UniProtKB/Swiss-Prot Function
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Resources
"NM_173598" in other vectors (7)
SKU Description Size Price
RC211665L1 Lenti ORF clone of Human kinase suppressor of ras 2 (KSR2), Myc-DDK-tagged 10 ug
$1,143.00
RC211665L2 Lenti ORF clone of Human kinase suppressor of ras 2 (KSR2), mGFP tagged 10 ug
$1,143.00
RC211665L3 Lenti ORF clone of Human kinase suppressor of ras 2 (KSR2), Myc-DDK-tagged 10 ug
$1,143.00
RC211665L4 Lenti ORF clone of Human kinase suppressor of ras 2 (KSR2), mGFP tagged 10 ug
$1,143.00
RG211665 KSR2 (tGFP-tagged) - Human kinase suppressor of ras 2 (KSR2) 10 ug
$789.00 $1,043.00
SC306873 KSR2 (untagged)-Human kinase suppressor of ras 2 (KSR2) 10 ug
$928.00
SC318393 KSR2 (untagged)-Human kinase suppressor of ras 2 (KSR2) 10 ug
$844.00

Plasmid Map for RC211665

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC211665
Circular map for RC211665

file-alt Citations

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