PRICKLE4 (NM_013397) Human Tagged ORF Clone

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SKU
RC211641
PRICKLE4 (Myc-DDK-tagged)-Human prickle homolog 4 (Drosophila) (PRICKLE4)
  $457.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol PRICKLE4
Synonyms C6orf49; OBTP; OEBT; TOMM6
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC211641 representing NM_013397
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCAGTGCAGAACTCTGGCTGGCCCCACCAAGAAGACAGCCCCAAGCCCCAGGATCCAGGTCCACCAG
CCAACTCAGACAGTGACTCAGGCCACCTGCCGGGGGAGGACCCTGAGGATACCCATGCTCAGGGTCCTGC
AGTTCTGAGCTTGGGTTCCCTTTGCCTGGACACCAACCAAGCCCCCAACTGGACTGGACTTCAGACCCTC
CTGCAGCAACTCCCTCCGCAGGACATTGATGAGCGCTACTGCCTGGCCCTTGGGGAGGAGGAGCGGGCCG
AGCTGCAGCTCTTCTGTGCCAGGCGGAAGCAGGAAGCCCTGGGACAGGGGGTAGCCCGCCTGGTACTTCC
CAAGCTTGAAGGACACACCTGTGAGAAGTGTAGGGAGCTGCTGAAGCCAGGGGAGTACGGAGTGTTTGCA
GCCCGGGCAGGGGAACAGCGCTGCTGGCACCAGCCTTGCTTTGCCTGCCAGGCCTGTGGCCAGGCCCTGA
TAAACCTCATCTACTTCTACCATGATGGACAACTCTACTGCGGCCGTCATCATGCAGAGTTGCTGCGCCC
GCGCTGCCCGGCTTGTGACCAGCTGATCTTCTCCTGGCGCTGCACCGAGGCGGAGGGACAGCGCTGGCAT
GAGAACCACTTCTGTTGCCAGGACTGCGCCGGGCCTCTGGGCGGGGGACGTTATGCCCTGCCTGGGGGAA
GCCCCTGCTGCCCCAGCTGCTTCGAGAACCGCTACTCGGATGCAGGCTCGAGCTGGGCCGGGGCACTGGA
AGGGCAGGCATTCCTTGGGGAGACTGGACTCGACCGAACTGAAGGAAGGGACCAAACCTCGGTGAACTCT
GCAACCCTCTCCCGAACACTCCTCGCTGCTGCCGGCGGTTCCAGCCTGCAAACTCAGAGGGGGCTGCCTG
GATCCAGTCCCCAGCAGGAGAACCGACCTGGGGACAAAGCGGAGGCACCCAAAGGGCAGGAGCAATGCCG
CCTGGAGACTATTCGTGATCCCAAGGACACCCCTTTCTCCACCTGCTCCTCCTCCTCTGACTCGGAACCT
GAAGGATTTTTCTTAGGCGAGCGCCTTCCCCAGTCCTGGAAGACCCCCGGAAGCCTCCAAGCAGAGGACA
GCAACGCCTCTAAGACGCACTGCACCATGTGC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC211641 representing NM_013397
Red=Cloning site Green=Tags(s)

MSVQNSGWPHQEDSPKPQDPGPPANSDSDSGHLPGEDPEDTHAQGPAVLSLGSLCLDTNQAPNWTGLQTL
LQQLPPQDIDERYCLALGEEERAELQLFCARRKQEALGQGVARLVLPKLEGHTCEKCRELLKPGEYGVFA
ARAGEQRCWHQPCFACQACGQALINLIYFYHDGQLYCGRHHAELLRPRCPACDQLIFSWRCTEAEGQRWH
ENHFCCQDCAGPLGGGRYALPGGSPCCPSCFENRYSDAGSSWAGALEGQAFLGETGLDRTEGRDQTSVNS
ATLSRTLLAAAGGSSLQTQRGLPGSSPQQENRPGDKAEAPKGQEQCRLETIRDPKDTPFSTCSSSSDSEP
EGFFLGERLPQSWKTPGSLQAEDSNASKTHCTMC

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_013397
ORF Size 1152 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_013397.6
RefSeq Size 2542 bp
RefSeq ORF 1155 bp
Locus ID 29964
UniProt ID Q2TBC4
Cytogenetics 6p21.1
MW 41.6 kDa
Summary C6ORF49 is a member of the LIM domain protein family (Teufel et al., 2005 PubMed 15702247).supplied by OMIM, Mar 2008
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Documents
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"NM_013397" in other vectors (5)
SKU Description Size Price
RC211641L3 Lenti ORF clone of Human prickle homolog 4 (Drosophila) (PRICKLE4), Myc-DDK-tagged 10 ug
$757.00
RC211641L4 Lenti ORF clone of Human prickle homolog 4 (Drosophila) (PRICKLE4), mGFP tagged 10 ug
$757.00
RG211641 PRICKLE4 (tGFP-tagged) - Human prickle homolog 4 (Drosophila) (PRICKLE4) 10 ug
$489.00 $657.00
SC115249 PRICKLE4 (untagged)-Human prickle homolog 4 (Drosophila) (PRICKLE4) 10 ug
$150.00
SC317615 PRICKLE4 (untagged)-Human prickle homolog 4 (Drosophila) (PRICKLE4) 10 ug
$503.00

Plasmid Map for RC211641

Download sequence for

Cloning scheme for RC211641

Circular map for RC211641

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