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PCDHB7 (NM_018940) Human Tagged ORF Clone

  • TrueORF®
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SKU
RC211592
PCDHB7 (Myc-DDK-tagged)-Human protocadherin beta 7 (PCDHB7)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$589.00 $726.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol PCDHB7
Synonyms PCDH-BETA7
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC211592 representing NM_018940
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGGCCAGAGTGGAGCGTGCTGTGCAGAAAAGGCAAGTCTTATTTCTTTGTGTATTTCTGGGAATGT
CTTGGGCTGGCGCCGAACCGCTTCGGTATTTTGTGGCGGAGGAAACCGAGAGAGGCACCTTTCTTACCAA
CTTGGCAAAAGACCTAGGGTTAGGGGTAGGGGAACTGAGAGCCCGGGGAACTAGAATTGTTTCAGACCAG
AACATGCAAATTTTACTGCTCAGTTCGCTTACTGGTGATCTACTTCTAAATGAGAAATTGGACCGAGAGG
AACTGTGTGGCCCCAGAGAGCCCTGTGTGCTGCCTTTCCAGTTGTTATTGGAAAAACCTTTTCAGATTTT
CCGTGCTGAACTATGGGTCAGAGACATCAATGATCACGCTCCAGTATTTCTAGACAGAGAGATTTCCTTG
AAAATATTAGAAAGTACCACTCCAGGGGCGGCATTTCTCCTAGAGAGTGCACAGGATTCAGATGTTGGAA
CCAACAGCCTGAGTAACTACACCATCAGCCCCAATGCCTATTTCCATATTAATGTCCATGATAGCGGGGA
GGGGAATATCTATCCCGAATTGGTGCTGAATCAAGTGCTGGATCGGGAAGAGATACCAGAGTTCAGTTTA
ACCCTCACCGCTTTAGACGGCGGCTCTCCTCCAAGATCAGGGACCGCCCTCGTGCGCATTCTGGTTCTAG
ACGTAAATGACAACGCCCCTGATTTTGTGCGGTCGCTCTACAAGGTGCAGGTGCCCGAAAATAGCCCCGT
TGGTTCCATGGTTGTCTCCGTGTCAGCCAGAGATTTAGATACCGGAAGTAATGGGGAAATAGCCTATGCA
TTTTCTTACGCCACTGAAAGAATTCTCAAAACGTTTCAAATCAATCCAACATCTGGCAGTCTTCATCTTA
AAGCGCAATTGGACTATGAGGCAATTCAAACTTACACATTAACTATTCAGGCCAAAGACGGCGGCGGGCT
TTCTGGAAAATGCACTGTAGTGGTTGATGTAACAGATATAAACGATAATCGACCCGAGCTGCTCCTGTCT
TCACTTACTAGCCCAATTGCAGAAAACTCACCCGAGACAGTCGTGGCTGTTTTTAGGATTAGAGACAGAG
ATTCCGGGAACAATGGAAAGACAGTGTGCTCCATCCAGGACGATGTCCCCTTCATCCTGAAGCCATCTGT
CGAAAACTTCTATACTCTGGTAACAGAGAAACCTTTGGATCGAGAGAGGAACACTGAGTACAACATCACC
ATCACCGTCACCGACTTGGGGACACCCAGGCTGAAAACCGAGCACAACATAACCGTGCTGGTCTCCGACG
TCAATGACAACGCTCCCGCCTTCACCCAAACCTCCTACACCCTGTTTGTCCGTGAGAACAACAGCCCCGC
CCTGCCCATCGGCAGTGTCAGCGCCACAGACAGAGACTCGGGCACCAACGCCCAGGTCATCTACTCCCTG
CTGCCGTCCCAGGACCCGCACCTGCCCCTCGCCTCCCTGGTCTCCATCAACGCGGACAACGGCCACCTGT
TTGCCCTCAGGTCCCTGGACTACGAGGCCCTGCAGGCGTTCGAGTTCCGCGTGGGCGCCACAGACCGCGG
CTCCCCCGCGCTGAGCAGCGAGGCGCTGGTGCGCGTGCTGGTGCTGGACGCCAACGACAACTCGCCCTTC
GTGCTGTACCCGCTGCAGAACAGCTCCGCGCCCTGCACCGAGCCGTTGCCCCGGGCGGCCGAGCCGGGCT
ACCTGGTGACCAAGGTGGTGGCGGTGGACGGCGACTCGGGCCAGAACGCCTGGCTGTCGTACCAGCTGCT
CAAGGCCACGGAGCCCGGGCTATTCGGCGTGTGGGCGCACAATGGCGAGGTGCGTACCGCCAGGCTGCTG
AGCGAGCGCGACGCAGCCAAGCAGAGGCTGGTGGTGCTGGTCAAGGACAATGGCGAGCCTCCGCGCTCGG
CCACCGCCACGCTGCACGTGCTCCTGGTGGACGGCTTCTCCCAGCCCTACCTGCGGCTCCCGGAGGCGGC
CCCGGACCAGGCCAACTCGCTCACCGTCTACCTGGTGGTGGCGTTGGCCTCGGTGTCTTCGCTCTTCCTC
CTCTCGGTGCTCCTGTTCGTGGCGGTGCGGCTGTGCAGGAGGAGCAGGGCGGCCCCGGTGGGTCGCTGCT
CGGTGCCTGAGGGCCCCTTTCCACGACATCTGGTGGACTTGAGCGGCACCGGGACCCTATCCCAGAGCTA
CCAGTATGAGGTGTGCCTGACTGGAGGCTCCGGGACAAATGAGTTCAAGTTTCTGAAACCAATTATCCCC
AACCTGCTACCCCAGAGCACAGGCAGGGAAGTGGAAGAAAATCGCCCATTTCAGAATAATTTGGGTTTC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC211592 representing NM_018940
Red=Cloning site Green=Tags(s)

MEARVERAVQKRQVLFLCVFLGMSWAGAEPLRYFVAEETERGTFLTNLAKDLGLGVGELRARGTRIVSDQ
NMQILLLSSLTGDLLLNEKLDREELCGPREPCVLPFQLLLEKPFQIFRAELWVRDINDHAPVFLDREISL
KILESTTPGAAFLLESAQDSDVGTNSLSNYTISPNAYFHINVHDSGEGNIYPELVLNQVLDREEIPEFSL
TLTALDGGSPPRSGTALVRILVLDVNDNAPDFVRSLYKVQVPENSPVGSMVVSVSARDLDTGSNGEIAYA
FSYATERILKTFQINPTSGSLHLKAQLDYEAIQTYTLTIQAKDGGGLSGKCTVVVDVTDINDNRPELLLS
SLTSPIAENSPETVVAVFRIRDRDSGNNGKTVCSIQDDVPFILKPSVENFYTLVTEKPLDRERNTEYNIT
ITVTDLGTPRLKTEHNITVLVSDVNDNAPAFTQTSYTLFVRENNSPALPIGSVSATDRDSGTNAQVIYSL
LPSQDPHLPLASLVSINADNGHLFALRSLDYEALQAFEFRVGATDRGSPALSSEALVRVLVLDANDNSPF
VLYPLQNSSAPCTEPLPRAAEPGYLVTKVVAVDGDSGQNAWLSYQLLKATEPGLFGVWAHNGEVRTARLL
SERDAAKQRLVVLVKDNGEPPRSATATLHVLLVDGFSQPYLRLPEAAPDQANSLTVYLVVALASVSSLFL
LSVLLFVAVRLCRRSRAAPVGRCSVPEGPFPRHLVDLSGTGTLSQSYQYEVCLTGGSGTNEFKFLKPIIP
NLLPQSTGREVEENRPFQNNLGF

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_018940
ORF Size 2379 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_018940.4
RefSeq Size 3715 bp
RefSeq ORF 2382 bp
Locus ID 56129
UniProt ID Q9Y5E2
Cytogenetics 5q31.3
Protein Families Transmembrane
MW 83.6 kDa
Summary This gene is a member of the protocadherin beta gene cluster, one of three related gene clusters tandemly linked on chromosome five. The gene clusters demonstrate an unusual genomic organization similar to that of B-cell and T-cell receptor gene clusters. The beta cluster contains 16 genes and 3 pseudogenes, each encoding 6 extracellular cadherin domains and a cytoplasmic tail that deviates from others in the cadherin superfamily. The extracellular domains interact in a homophilic manner to specify differential cell-cell connections. Unlike the alpha and gamma clusters, the transcripts from these genes are made up of only one large exon, not sharing common 3' exons as expected. These neural cadherin-like cell adhesion proteins are integral plasma membrane proteins. Their specific functions are unknown but they most likely play a critical role in the establishment and function of specific cell-cell neural connections. The transcript for this particular family member uses more than one polyadenylation site. provided by RefSeq, Jul 2008
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"NM_018940" in other vectors (4)
SKU Description Size Price
RC211592L3 Lenti-ORF clone of PCDHB7 (Myc-DDK-tagged)-Human protocadherin beta 7 (PCDHB7) 10 ug
$1,026.00
RC211592L4 Lenti-ORF clone of PCDHB7 (mGFP-tagged)-Human protocadherin beta 7 (PCDHB7) 10 ug
$1,026.00
RG211592 PCDHB7 (tGFP-tagged) - Human protocadherin beta 7 (PCDHB7) 10 ug
$926.00
SC304637 PCDHB7 (untagged)-Human protocadherin beta 7 (PCDHB7) 10 ug
$727.00

Plasmid Map for RC211592

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC211592
Circular map for RC211592

file-alt Citations

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