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RHOBTB1 (NM_198225) Human Tagged ORF Clone

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SKU
RC211541
RHOBTB1 (Myc-DDK-tagged)-Human Rho-related BTB domain containing 1 (RHOBTB1), transcript variant 2
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $637.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol RHOBTB1
Synonyms 1700008H16Rik; 3110048G13Rik; AI173445; AI573858; AV350930; KIAA0740, MGC33059, MGC33841; MGC107538; Rho-related BTB domain containing 1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC211541 representing NM_198225
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGACGCTGACATGGACTACGAAAGACCCAACGTTGAAACTATCAAATGTGTGGTCGTGGGTGACAATG
CCGTGGGGAAGACGCGCTTGATCTGTGCCAGGGCGTGCAACACCACACTCACGCAGTATCAGCTGCTGGC
CACCCACGTGCCAACAGTGTGGGCGATTGACCAGTACCGCGTGTGCCAGGAGGTCTTGGAGCGTTCTCGG
GATGTTGTTGATGAAGTGAGTGTTTCTCTCAGGCTTTGGGATACTTTTGGTGATCATCACAAAGACAGAC
GCTTTGCATATGGCAGGTCTGATGTTGTGGTCCTCTGTTTTTCGATTGCTAATCCCAATTCCCTAAATCA
TGTGAAAAGCATGTGGTATCCAGAAATCAAGCACTTTTGCCCTCGAACACCCGTTATCCTTGTTGGGTGC
CAGCTTGATCTCCGCTATGCCGACCTGGAAGCTGTTAATCGAGCCAGGCGCCCGTTAGCAAGGCCCATAA
AGAGAGGGGATATTTTGCCCCCAGAAAAAGGCCGAGAGGTAGCAAAGGAACTTGGCTTACCATACTATGA
AACAAGCGTGTTTGACCAGTTTGGTATCAAGGATGTGTTTGACAATGCAATCCGAGCAGCGCTGATTTCC
CGCAGGCACCTGCAATTCTGGAAATCCCACCTAAAGAAAGTCCAGAAACCTTTACTTCAGGCACCCTTCC
TACCTCCAAAAGCCCCTCCACCGGTCATCAAAATTCCAGAGTGTCCTTCCATGGGGACAAATGAAGCTGC
CTGTTTACTGGACAATCCTCTATGTGCCGATGTTCTGTTCATCCTTCAGGACCAGGAACACATCTTTGCA
CATCGAATTTACCTCGCTACCTCTTCTTCCAAATTTTATGATCTGTTTTTAATGGAATGTGAAGAATCCC
CAAATGGGAGTGAAGGAGCCTGTGAGAAAGAGAAGCAGAGCAGAGATTTCCAGGGGCGGATATTGAGTGT
CGACCCAGAGGAAGAAAGGGAGGAGGGCCCGCCTAGGATTCCTCAGGCCGACCAGTGGAAGTCTTCAAAC
AAGAGCCTGGTGGAGGCTCTGGGGCTGGAAGCCGAGGGTGCAGTTCCTGAGACACAGACTTTGACCGGAT
GGAGTAAGGGGTTCATTGGCATGCACAGGGAAATGCAAGTCAACCCCATTTCAAAGCGGATGGGGCCCAT
GACTGTGGTCAGGATGGACGCTTCAGTCCAGCCAGGCCCTTTTCGGACCCTGCTCCAGTTTCTTTATACG
GGACAACTGGATGAAAAGGAAAAGGATTTGGTGGGCCTGGCTCAGATCGCAGAGGTCCTCGAGATGTTCG
ATTTGAGGATGATGGTGGAAAACATCATGAACAAGGAAGCCTTCATGAACCAGGAGATTACGAAAGCCTT
TCACGTAAGGAAAGCCAATCGGATAAAAGAGTGTCTCAGCAAGGGAACGTTCTCGGACGTGACATTTAAA
TTGGACGATGGAGCCATCAGTGCCCACAAGCCGCTGCTGATCTGTAGCTGTGAGTGGATGGCAGCCATGT
TCGGGGGGTCATTTGTGGAAAGTGCCAACAGTGAGGTGTATCTCCCGAACATAAACAAGATATCAATGCA
AGCAGTATTGGATTATCTCTATACCAAGCAGTTGTCTCCTAACTTGGATCTGGACCCGCTGGAATTAATT
GCCTTGGCAAACAGATTTTGCCTGCCACACTTGGTTGCACTTGCAGAACAGCATGCCGTTCAGGAGTTGA
CCAAAGCCGCCACGAGTGGCGTGGGCATTGACGGAGAAGTGCTCTCTTACTTGGAATTGGCTCAGTTTCA
CAATGCCCACCAGTTGGCCGCCTGGTGTTTGCACCACATCTGCACCAACTACAACAGTGTATGCTCCAAG
TTCCGTAAGGAAATCAAATCAAAATCTGCAGACAACCAGGAATACTTCGAGCGGCACCGCTGGCCCCCTG
TGTGGTACCTGAAGGAAGAAGATCACTACCAGCGTGTGAAAAGGGAACGAGAGAAGGAAGATATTGCACT
AAATAAGCATCGCTCAAGACGAAAGTGGTGCTTCTGGAATTCATCTCCAGCAGTGGCC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC211541 representing NM_198225
Red=Cloning site Green=Tags(s)

MDADMDYERPNVETIKCVVVGDNAVGKTRLICARACNTTLTQYQLLATHVPTVWAIDQYRVCQEVLERSR
DVVDEVSVSLRLWDTFGDHHKDRRFAYGRSDVVVLCFSIANPNSLNHVKSMWYPEIKHFCPRTPVILVGC
QLDLRYADLEAVNRARRPLARPIKRGDILPPEKGREVAKELGLPYYETSVFDQFGIKDVFDNAIRAALIS
RRHLQFWKSHLKKVQKPLLQAPFLPPKAPPPVIKIPECPSMGTNEAACLLDNPLCADVLFILQDQEHIFA
HRIYLATSSSKFYDLFLMECEESPNGSEGACEKEKQSRDFQGRILSVDPEEEREEGPPRIPQADQWKSSN
KSLVEALGLEAEGAVPETQTLTGWSKGFIGMHREMQVNPISKRMGPMTVVRMDASVQPGPFRTLLQFLYT
GQLDEKEKDLVGLAQIAEVLEMFDLRMMVENIMNKEAFMNQEITKAFHVRKANRIKECLSKGTFSDVTFK
LDDGAISAHKPLLICSCEWMAAMFGGSFVESANSEVYLPNINKISMQAVLDYLYTKQLSPNLDLDPLELI
ALANRFCLPHLVALAEQHAVQELTKAATSGVGIDGEVLSYLELAQFHNAHQLAAWCLHHICTNYNSVCSK
FRKEIKSKSADNQEYFERHRWPPVWYLKEEDHYQRVKREREKEDIALNKHRSRRKWCFWNSSPAVA

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_198225
ORF Size 2088 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_198225.1, NP_937868.1
RefSeq Size 4382 bp
RefSeq ORF 2090 bp
Locus ID 9886
Cytogenetics 10q21.2
MW 79.2 kDa
Summary The protein encoded by this gene belongs to the Rho family of the small GTPase superfamily. It contains a GTPase domain, a proline-rich region, a tandem of 2 BTB (broad complex, tramtrack, and bric-a-brac) domains, and a conserved C-terminal region. The protein plays a role in small GTPase-mediated signal transduction and the organization of the actin filament system. Alternate splicing results in multiple transcript variants. provided by RefSeq, Dec 2008
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"NM_198225" in other vectors (4)
SKU Description Size Price
RC211541L3 Lenti ORF clone of Human Rho-related BTB domain containing 1 (RHOBTB1), transcript variant 2, Myc-DDK-tagged 10 ug
$937.00
RC211541L4 Lenti ORF clone of Human Rho-related BTB domain containing 1 (RHOBTB1), transcript variant 2, mGFP tagged 10 ug
$937.00
RG211541 RHOBTB1 (tGFP-tagged) - Human Rho-related BTB domain containing 1 (RHOBTB1), transcript variant 2 10 ug
$789.00 $837.00
SC107799 RHOBTB1 (untagged)-Human Rho-related BTB domain containing 1 (RHOBTB1), transcript variant 2 10 ug
$638.00

Plasmid Map for RC211541

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC211541
Circular map for RC211541

file-alt Citations

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