CABP4 (NM_145200) Human Tagged ORF Clone

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SKU
RC211536
CABP4 (Myc-DDK-tagged)-Human calcium binding protein 4 (CABP4)
  $300.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol CABP4
Synonyms CRSD; CSNB2B
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC211536 representing NM_145200
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGACCACAGAGCAGGCAAGGGGGCAGCAGGGCCCAAATCTGGCCATTGGCCGTCAGAAGCCCCCTGCGG
GGGTTGTGACTCCCAAGAGTGATGCAGAGGAGCCCCCGTTGACCAGGAAGAGGAGCAAGAAGGAGAGGGG
GCTCCGAGGGTCTCGAAAGCGCACTGGCAGCTCTGGGGAGCAGACAGGCCCCGAGGCCCCGGGGAGCAGC
AATAACCCTCCCAGCACTGGAGAGGGGCCGGCGGGCGCACCCCCTGCATCCCCTGGGCCGGCCTCTTCTC
GCCAGTCCCACCGACATCGTCCTGACTCCCTGCACGACGCTGCTCAGAGGACATACGGGCCCCTGCTCAA
TCGAGTCTTCGGGAAGGACCGCGAACTGGGCCCCGAGGAGCTAGACGAGCTTCAGGCCGCCTTCGAGGAG
TTTGACACTGACCGTGACGGCTACATCAGCCACCGGGAGCTGGGTGACTGCATGCGGACCCTGGGCTACA
TGCCCACCGAGATGGAGCTCCTGGAGGTCTCGCAGCACATCAAGATGCGCATGGGCGGCCGTGTGGACTT
TGAGGAGTTTGTAGAACTGATAGGCCCAAAGCTGAGGGAGGAGACGGCGCACATGCTGGGGGTGCGAGAG
CTGCGCATCGCCTTCCGAGAGTTTGACAGGGACAGGGATGGACGAATTACGGTGGCGGAGCTGCGGGAGG
CGGTACCGGCTCTGCTCGGGGAGCCGCTGGCGGGTCCTGAGCTGGACGAGATGCTCCGAGAAGTGGACCT
CAATGGGGATGGCACCGTAGACTTTGACGAGTTTGTGATGATGCTCTCCCGCCAC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC211536 representing NM_145200
Red=Cloning site Green=Tags(s)

MTTEQARGQQGPNLAIGRQKPPAGVVTPKSDAEEPPLTRKRSKKERGLRGSRKRTGSSGEQTGPEAPGSS
NNPPSTGEGPAGAPPASPGPASSRQSHRHRPDSLHDAAQRTYGPLLNRVFGKDRELGPEELDELQAAFEE
FDTDRDGYISHRELGDCMRTLGYMPTEMELLEVSQHIKMRMGGRVDFEEFVELIGPKLREETAHMLGVRE
LRIAFREFDRDRDGRITVAELREAVPALLGEPLAGPELDEMLREVDLNGDGTVDFDEFVMMLSRH

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_145200
ORF Size 825 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_145200.5
RefSeq Size 1466 bp
RefSeq ORF 828 bp
Locus ID 57010
UniProt ID P57796
Cytogenetics 11q13.2
MW 30.3 kDa
Summary This gene encodes a member of the CABP family of calcium binding protein characterized by four EF-hand motifs. Mutations in this gene are associated with congenital stationary night blindness type 2B. Three transcript variants encoding two different isoforms have been found for this gene. provided by RefSeq, Jul 2014
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Documents
Resources
"NM_145200" in other vectors (4)
SKU Description Size Price
RC211536L3 Lenti ORF clone of Human calcium binding protein 4 (CABP4), Myc-DDK-tagged 10 ug
$600.00
RC211536L4 Lenti ORF clone of Human calcium binding protein 4 (CABP4), mGFP tagged 10 ug
$600.00
RG211536 CABP4 (tGFP-tagged) - Human calcium binding protein 4 (CABP4) 10 ug
$489.00 $500.00
SC306234 CABP4 (untagged)-Human calcium binding protein 4 (CABP4) 10 ug
$300.00

Plasmid Map for RC211536

Download sequence for

Cloning scheme for RC211536

Circular map for RC211536

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