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ADAM18 (NM_014237) Human Tagged ORF Clone

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SKU
RC211531
ADAM18 (Myc-DDK-tagged)-Human ADAM metallopeptidase domain 18 (ADAM18), transcript variant 1
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $677.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol ADAM18
Synonyms ADAM27; tMDCIII
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC211531 representing NM_014237
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTTCCTTCTCCTCGCCCTCCTCACTGAGCTTGGAAGACTGCAAGCCCACGAAGGTTCTGAAGGAATAT
TTCTGCATGTCACAGTTCCACGGAAGATTAAGTCAAATGACAGTGAAGTTTCAGAGAGGAAGATGATTTA
CATCATTACAATTGATGGACAACCTTACACTCTACATCTCGGAAAACAATCATTCTTACCCCAGAACTTT
TTGGTTTATACATATAATGAAACTGGATCTTTGCATTCTGTGTCTCCATATTTTATGATGCATTGCCATT
ACCAAGGATATGCTGCCGAATTTCCAAATTCATTTGTGACACTCAGTATATGTTCTGGTCTCAGGGGATT
TCTCCAGTTTGAAAATATCAGTTATGGAATTGAACCAGTAGAATCTTCAGCAAGATTTGAGCATATAATT
TATCAAATGAAAAATAATGATCCAAATGTATCCATTTTAGCAGTAAATTACAGTCATATTTGGCAGAAAG
ACCAGCCCTACAAAGTTCCTTTAAACTCACAGATAAAAAATCTTTCAAAACTATTACCCCAATATCTGGA
AATATACATTATAGTGGAAAAAGCTTTGTATGATTATATGGGATCTGAAATGATGGCTGTAACACAAAAA
ATTGTCCAGGTTATTGGGCTTGTCAACACTATGTTTACCCAGTTCAAATTGACTGTTATACTGTCTTCCT
TGGAATTGTGGTCAAATGAAAACCAGATTTCCACCAGTGGGGATGCTGATGATATATTACAAAGATTTTT
GGCATGGAAACGGGACTATCTCATCCTACGGCCCCATGACATAGCATACTTACTTGTTTACAGGAAACAT
CCTAAATATGTGGGAGCAACATTTCCTGGCACTGTATGCAATAAAAGCTATGATGCAGGTATTGCTATGT
ATCCAGATGCAATAGGTTTGGAGGGATTTTCGGTTATTATAGCTCAACTGCTTGGCCTTAATGTAGGATT
AACATATGATGACATCACTCAGTGTTTCTGTCTGAGAGCTACATGCATCATGAATCATGAAGCAGTGAGT
GCCAGTGGTAGAAAGATTTTTAGCAACTGCAGCATGCACGACTATAGATATTTTGTTTCAAAATTTGAGA
CTAAATGCCTTCAGAAGCTTTCAAATTTGCAACCATTACATCAAAATCAACCAGTGTGTGGTAATGGGAT
TTTGGAATCCAATGAAGAATGTGACTGTGGTAATAAAAATGAATGTCAATTTAAGAAGTGCTGTGATTAT
AACACATGTAAACTGAAGGGCTCAGTAAAATGTGGTTCTGGACCATGTTGTACATCAAAGTGTGAGTTGT
CAATAGCAGGCACTCCATGTAGAAAGAGTATTGATCCAGAGTGTGATTTTACAGAGTACTGCAATGGAAC
CTCTAGTAATTGTGTTCCTGACACTTATGCATTGAATGGCCGTTTGTGCAAGTTGGGAACTGCCTATTGC
TATAACGGACAATGTCAAACTACTGATAACCAGTGTGCCAAGATATTTGGAAAAGGTGCTCAAGGTGCTC
CATTTGCCTGTTTTAAAGAAGTTAATTCTCTGCATGAAAGATCTGAAAACTGTGGTTTTAAAAATTCACA
ACCATTACCTTGTGAACGGAAGGATGTTCTCTGTGGAAAATTAGCTTGTGTTCAGCCACATAAAAATGCT
AATAAAAGTGACGCTCAATCTACAGTTTATTCATATATTCAAGACCATGTATGTGTATCTATAGCCACTG
GTTCCTCCATGAGATCAGATGGAACAGACAATGCCTATGTGGCTGATGGCACCATGTGTGGTCCAGAAAT
GTACTGTGTAAATAAAACCTGCAGAAAAGTTCATTTAATGGGATATAACTGTAATGCCACCACAAAATGC
AAAGGGAAAGGGATATGTAATAATTTTGGTAATTGTCAATGCTTCCCTGGACATAGACCTCCAGATTGTA
AATTCCAGTTTGGTTCCCCAGGGGGTAGTATTGATGATGGAAATTTTCAGAAATCTGGTGACTTTTATAC
TGAAAAAGGCTACAATACACACTGGAACAACTGGTTTATTCTGAGTTTCTGCATTTTTCTGCCGTTTTTC
ATAGTTTTCACCACTGTGATCTTTAAAAGAAATGAAATAAGTAAATCATGTAACAGAGAGAATGCAGAGT
ATAATCGTAATTCATCCGTTGTATCAGAAAGCGATGACGTGGGACAT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC211531 representing NM_014237
Red=Cloning site Green=Tags(s)

MFLLLALLTELGRLQAHEGSEGIFLHVTVPRKIKSNDSEVSERKMIYIITIDGQPYTLHLGKQSFLPQNF
LVYTYNETGSLHSVSPYFMMHCHYQGYAAEFPNSFVTLSICSGLRGFLQFENISYGIEPVESSARFEHII
YQMKNNDPNVSILAVNYSHIWQKDQPYKVPLNSQIKNLSKLLPQYLEIYIIVEKALYDYMGSEMMAVTQK
IVQVIGLVNTMFTQFKLTVILSSLELWSNENQISTSGDADDILQRFLAWKRDYLILRPHDIAYLLVYRKH
PKYVGATFPGTVCNKSYDAGIAMYPDAIGLEGFSVIIAQLLGLNVGLTYDDITQCFCLRATCIMNHEAVS
ASGRKIFSNCSMHDYRYFVSKFETKCLQKLSNLQPLHQNQPVCGNGILESNEECDCGNKNECQFKKCCDY
NTCKLKGSVKCGSGPCCTSKCELSIAGTPCRKSIDPECDFTEYCNGTSSNCVPDTYALNGRLCKLGTAYC
YNGQCQTTDNQCAKIFGKGAQGAPFACFKEVNSLHERSENCGFKNSQPLPCERKDVLCGKLACVQPHKNA
NKSDAQSTVYSYIQDHVCVSIATGSSMRSDGTDNAYVADGTMCGPEMYCVNKTCRKVHLMGYNCNATTKC
KGKGICNNFGNCQCFPGHRPPDCKFQFGSPGGSIDDGNFQKSGDFYTEKGYNTHWNNWFILSFCIFLPFF
IVFTTVIFKRNEISKSCNRENAEYNRNSSVVSESDDVGH

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_014237
ORF Size 2217 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_014237.3
RefSeq Size 2248 bp
RefSeq ORF 2220 bp
Locus ID 8749
UniProt ID Q9Y3Q7
Cytogenetics 8p11.22
Protein Families Druggable Genome, Protease, Transmembrane
MW 82.8 kDa
Summary This gene encodes a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biologic processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. The encoded preproprotein is proteolytically processed to generate the mature sperm surface protein. Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed. provided by RefSeq, Feb 2016
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Documents
Resources
"NM_014237" in other vectors (4)
SKU Description Size Price
RC211531L3 Lenti-ORF clone of ADAM18 (Myc-DDK-tagged)-Human ADAM metallopeptidase domain 18 (ADAM18), transcript variant 1 10 ug
$977.00
RC211531L4 Lenti-ORF clone of ADAM18 (mGFP-tagged)-Human ADAM metallopeptidase domain 18 (ADAM18), transcript variant 1 10 ug
$977.00
RG211531 ADAM18 (tGFP-tagged) - Human ADAM metallopeptidase domain 18 (ADAM18), transcript variant 1 10 ug
$877.00
SC125245 ADAM18 (untagged)-Human ADAM metallopeptidase domain 18 (ADAM18), transcript variant 1 10 ug
$678.00

Plasmid Map for RC211531

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC211531
Circular map for RC211531

file-alt Citations

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