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OSBPL9 (NM_148909) Human Tagged ORF Clone

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SKU
RC211520
OSBPL9 (Myc-DDK-tagged)-Human oxysterol binding protein-like 9 (OSBPL9), transcript variant 7
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $683.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol OSBPL9
Synonyms ORP-9; ORP9
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC211520 representing NM_148909
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGTCCATCATGGAAGGGCCGCTGAGCAAATGGACTAACGTGATGAAGGGCTGGCAGTACCGTTGGT
TCGTGCTGGACTACAATGCAGGACTGCTCTCCTACTACACGTCCAAGGACAAAATGATGAGAGGCTCTCG
CAGAGGATGTGTTAGACTCAGAGGAGCTGTGATTGGTATAGACGATGAGGACGACAGCACCTTCACAATA
ACTGTTGATCAGAAAACCTTCCATTTCCAGGCCCGTGATGCTGATGAGCGAGAGAAGTGGATCCATGCCT
TAGAAGAAACAATTCTTCGACATACTCTCCAGCTTCAAATCAGTACCACACTTGCTTTTTTCCAGTCTTC
TGGTATCTCTCCAGTTCTTGAATTTTCAAAAATAATTGGTTTGGATTCAGGATTTGTTCCTAGTGTCCAA
GATTTTGATAAGAAACTTACAGAAGCTGATGCTTACCTACAAATCTTGATTGAACAATTAAAGCTTTTTG
ATGACAAGCTTCAAAACTGCAAAGAAGATGAACAGAGAAAGAAAATTGAAACTCTCAAAGAGACAACAAA
TAGCATGGTAGAATCAATTAAACACTGCATTGTGTTGCTGCAGATTGCCAAAAGTACTATTAATCCCGTA
GATGCAATATATCAACCTAGTCCTTTGGAACCTGTGATCAGCACAATGCCTTCCCAGACTGTGTTACCTC
CAGAACCTGTTCAGTTGTGTAAGTCAGAGCAGCGTCCATCTTCCCTACCAGTTGGACCTGTGTTGGCTAC
CTTGGGACATCATCAGACTCCTACACCAAATAGTACAGGCAGTGGCCATTCACCACCGAGTAGCAGTCTC
ACTTCTCCAAGCCACGTGAACTTGTCTCCAAATACAGTCCCAGAGTTCTCTTACTCCAGCAGTGAAGATG
AATTTTATGATGCTGATGAATTCCATCAAAGTGGCTCATCCCCAAAGCGCTTAATAGATTCTTCTGGATC
TGCCTCAGTCCTGACACACAGCAGCTCGGGAAATAGTCTAAAACGCCCAGATACCACAGAATCACTTAAT
TCTTCCTTGTCCAATGGAACAAGTGATGCTGACCTGTTTGATTCACATGATGACAGAGATGATGATGCGG
AGGCAGGGTCTGTGGAGGAGCACAAGAGCGTTATCATGCATCTCTTGTCGCAGGTTAGACTTGGAATGGA
TCTTACTAAGGTAGTTCTTCCAACGTTTATTCTTGAAAGAAGATCTCTTTTAGAAATGTATGCAGACTTT
TTTGCACATCCGGACCTGTTTGTGAGCATTAGTGACCAGAAGGATCCCAAGGATCGAATGGTTCAGGTTG
TGAAATGGTACCTCTCAGCCTTTCATGCGGGAAGGAAAGGATCAGTTGCCAAAAAGCCATACAATCCCAT
TTTGGGCGAGATTTTTCAGTGTCATTGGACATTACCAAATGATACTGAAGAGAACACAGAACTAGTTTCA
GAAGGACCAGTTCCCTGGGTTTCCAAAAACAGTGTAACATTTGTGGCTGAGCAGGTTTCCCATCATCCAC
CCATTTCAGCCTTTTATGCTGAGTGTTTTAACAAGAAGATACAATTCAATGCTCATATCTGGACCAAATC
AAAATTCCTTGGGATGTCAATTGGGGTGCACAACATAGGGCAGGGCTGTGTCTCATGTCTAGACTATGAT
GAACATTACATTCTCACATTCCCCAATGGCTATGGAAGGTCTATCCTCACAGTGCCCTGGGTGGAATTAG
GAGGAGAATGCAATATTAATTGTTCCAAAACAGGCTATAGTGCAAATATCATCTTCCACACTAAACCCTT
CTATGGGGGCAAGAAGCACAGAATTACTGCCGAGATTTTTTCTCCAAATGACAAGAAGTCTTTTTGCTCA
ATTGAAGGGGAATGGAATGGTGTGATGTATGCAAAATATGCAACAGGGGAAAATACAGTCTTTGTAGATA
CCAAGAAGTTGCCTATAATCAAGAAGAAAGTGAGGAAGTTGGAAGATCAGAACGAGTATGAATCCCGCAG
CCTTTGGAAGGATGTCACTTTCAACTTAAAAATCAGAGACATTGATGCAGCAACTGAAGCAAAGCACAGG
CTTGAAGAAAGACAAAGAGCAGAAGCCCGAGAAAGGAAGGAGAAGGAAATTCAGTGGGAGACAAGGTTAT
TTCATGAAGATGGAGAATGCTGGGTTTATGATGAACCATTACTGAAACGTCTTGGTGCTGCCAAGCAT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC211520 representing NM_148909
Red=Cloning site Green=Tags(s)

MASIMEGPLSKWTNVMKGWQYRWFVLDYNAGLLSYYTSKDKMMRGSRRGCVRLRGAVIGIDDEDDSTFTI
TVDQKTFHFQARDADEREKWIHALEETILRHTLQLQISTTLAFFQSSGISPVLEFSKIIGLDSGFVPSVQ
DFDKKLTEADAYLQILIEQLKLFDDKLQNCKEDEQRKKIETLKETTNSMVESIKHCIVLLQIAKSTINPV
DAIYQPSPLEPVISTMPSQTVLPPEPVQLCKSEQRPSSLPVGPVLATLGHHQTPTPNSTGSGHSPPSSSL
TSPSHVNLSPNTVPEFSYSSSEDEFYDADEFHQSGSSPKRLIDSSGSASVLTHSSSGNSLKRPDTTESLN
SSLSNGTSDADLFDSHDDRDDDAEAGSVEEHKSVIMHLLSQVRLGMDLTKVVLPTFILERRSLLEMYADF
FAHPDLFVSISDQKDPKDRMVQVVKWYLSAFHAGRKGSVAKKPYNPILGEIFQCHWTLPNDTEENTELVS
EGPVPWVSKNSVTFVAEQVSHHPPISAFYAECFNKKIQFNAHIWTKSKFLGMSIGVHNIGQGCVSCLDYD
EHYILTFPNGYGRSILTVPWVELGGECNINCSKTGYSANIIFHTKPFYGGKKHRITAEIFSPNDKKSFCS
IEGEWNGVMYAKYATGENTVFVDTKKLPIIKKKVRKLEDQNEYESRSLWKDVTFNLKIRDIDAATEAKHR
LEERQRAEARERKEKEIQWETRLFHEDGECWVYDEPLLKRLGAAKH

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_148909
ORF Size 2238 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_148909.4
RefSeq Size 2949 bp
RefSeq ORF 2241 bp
Locus ID 114883
UniProt ID Q96SU4, Q8TAS8
Cytogenetics 1p32.3
MW 84.1 kDa
Summary This gene encodes a member of the oxysterol-binding protein (OSBP) family, a group of intracellular lipid receptors. Most members contain an N-terminal pleckstrin homology domain and a highly conserved C-terminal OSBP-like sterol-binding domain, although some members contain only the sterol-binding domain. This family member functions as a cholesterol transfer protein that regulates Golgi structure and function. Multiple transcript variants, most of which encode distinct isoforms, have been identified. Related pseudogenes have been identified on chromosomes 3, 11 and 12. provided by RefSeq, Jul 2010
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"NM_148909" in other vectors (6)
SKU Description Size Price
RC211520L1 Lenti ORF clone of Human oxysterol binding protein-like 9 (OSBPL9), transcript variant 7, Myc-DDK-tagged 10 ug
$983.00
RC211520L2 Lenti ORF clone of Human oxysterol binding protein-like 9 (OSBPL9), transcript variant 7, mGFP tagged 10 ug
$983.00
RC211520L3 Lenti ORF clone of Human oxysterol binding protein-like 9 (OSBPL9), transcript variant 7, Myc-DDK-tagged 10 ug
$983.00
RC211520L4 Lenti ORF clone of Human oxysterol binding protein-like 9 (OSBPL9), transcript variant 7, mGFP tagged 10 ug
$983.00
RG211520 OSBPL9 (tGFP-tagged) - Human oxysterol binding protein-like 9 (OSBPL9), transcript variant 7 10 ug
$883.00
SC314600 OSBPL9 (untagged)-Human oxysterol binding protein-like 9 (OSBPL9), transcript variant 7 10 ug
$752.00

Plasmid Map for RC211520

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC211520
Circular map for RC211520

file-alt Citations

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