CCDC40 (NM_017950) Human Tagged ORF Clone

  • TrueORF®
SKU
RC211449
CCDC40 (Myc-DDK-tagged)-Human coiled-coil domain containing 40 (CCDC40)
$289.00 $457.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol CCDC40
Synonyms CFAP172; CILD15; FAP172
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC211449 representing NM_017950
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGAACCGGGCGGCGCGGCGGGCCGGTCCCATCCGGAAGATGGATCGGCTTCTGAGGGAGAGAAGG
AAGGGAATAATGAAAGCCACATGGTGTCACCACCAGAGAAGGATGATGGCCAGAAAGGTGAAGAAGCTGT
CGGTAGCACAGAGCATCCTGAGGAAGTCACAACCCAAGCGGAAGCTGCAATTGAAGAGGGGGAGGTCGAG
ACAGAAGGGGAAGCAGCAGTGGAAGGGGAAGAGGAGGCTGTGTCCTATGGAGATGCTGAAAGCGAAGAGG
AATATTACTATACAGAAACTTCATCCCCGGAAGGGCAAATCAGTGCTGCAGATACGACTTACCCGTATTT
CAGTCCTCCTCAGGAACTGCCTGGAGAGGAGGCATACGATAGTGTTAGCGGGGAGGCTGGTCTCCAAGGC
TTCCAGCAAGAGGCCACCGGTCCACCAGAATCCAGAGAAAGGAGGGTCACCTCCCCAGAGCCATCCCACG
GAGTCTTAGGCCCGTCGGAGCAAATGGGCCAGGTCACCTCTGGGCCAGCAGTGGGCAGATTGACAGGATC
CACAGAGGAGCCCCAGGGGCAGGTGCTCCCAATGGGCGTCCAGCACCGCTTCCGGCTGAGCCACGGGAGC
GACATCGAGTCCTCAGACCTGGAGGAGTTCGTCTCGCAGGAGCCAGTGATCCCCCCAGGGGTGCCCGATG
CCCACCCCAGGGAAGGAGACCTGCCAGTGTTCCAGGACCAGATCCAGCAGCCCAGCACCGAGGAGGGGGC
CATGGCAGAGAGAGTGGAGTCCGAGGGGAGTGACGAGGAAGCAGAAGACGAAGGGTCCCAGCTGGTGGTT
TTGGACCCAGACCACCCCCTGATGGTAAGATTCCAGGCTGCCCTGAAGAACTACCTGAACCGACAGATCG
AAAAGTTGAAGCTGGACCTCCAAGAGCTGGTTGTGGCTACCAAGCAGAGCCGAGCCCAGCGGCAGGAGCT
GGGGGTGAATCTCTATGAGGTGCAGCAGCACCTGGTACACCTGCAGAAGCTGCTGGAGAAGAGTCACGAC
CGCCACGCAATGGC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC211449 representing NM_017950
Red=Cloning site Green=Tags(s)

MAEPGGAAGRSHPEDGSASEGEKEGNNESHMVSPPEKDDGQKGEEAVGSTEHPEEVTTQAEAAIEEGEVE
TEGEAAVEGEEEAVSYGDAESEEEYYYTETSSPEGQISAADTTYPYFSPPQELPGEEAYDSVSGEAGLQG
FQQEATGPPESRERRVTSPEPSHGVLGPSEQMGQVTSGPAVGRLTGSTEEPQGQVLPMGVQHRFRLSHGS
DIESSDLEEFVSQEPVIPPGVPDAHPREGDLPVFQDQIQQPSTEEGAMAERVESEGSDEEAEDEGSQLVV
LDPDHPLMVRFQAALKNYLNRQIEKLKLDLQELVVATKQSRAQRQELGVNLYEVQQHLVHLQKLLEKSHD
RHAM

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_017950
ORF Size 1064 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq Size 4297 bp
RefSeq ORF 3429 bp
Locus ID 55036
UniProt ID Q4G0X9
Cytogenetics 17q25.3
MW 38.51 kDa
Summary This gene encodes a protein that is necessary for motile cilia function. It functions in correct left-right axis formation by regulating the assembly of the inner dynein arm and the dynein regulatory complexes, which control ciliary beat. Mutations in this gene cause ciliary dyskinesia type 15, a disorder due to defects in cilia motility. Alternative splicing results in multiple transcript variants. provided by RefSeq, Aug 2011
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Documents
Resources
"NM_017950" in other vectors (4)
SKU Description Size Price
RC211449L3 Lenti ORF clone of Human coiled-coil domain containing 40 (CCDC40), Myc-DDK-tagged 10 ug
$757.00
RC211449L4 Lenti ORF clone of Human coiled-coil domain containing 40 (CCDC40), mGFP tagged 10 ug
$757.00
RG211449 CCDC40 (tGFP-tagged) - Human coiled-coil domain containing 40 (CCDC40) 10 ug
$657.00
SC318521 CCDC40 (untagged)-Human coiled-coil domain containing 40 (CCDC40) 10 ug
$1,094.00

Plasmid Map for RC211449

Download sequence for

Cloning scheme for RC211449

Circular map for RC211449

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