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MDFIC (NM_199072) Human Tagged ORF Clone

  • TrueORF®
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SKU
RC211395
MDFIC (Myc-DDK-tagged)-Human MyoD family inhibitor domain containing (MDFIC), transcript variant 1
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $457.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "MDFIC or NM_199072"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol MDFIC
Synonyms HIC; MDFIC1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC211395 representing NM_199072.
Blue=ORF Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
GTGCGCGGAGTCAGAGCCGCCACCGCTGCCGCAGTTGCCGCCACTGCGGCGTCTGGGCTGAGCCGGAGG
GAGGCGGGAGGACGCGCAGGGGCGGCCGCCGCCGTCGTCAGGCCACCGGGGCGAAAATGCGGCCGCTGC
CGGAGGCTCGCTAACTTTCCGGGGCGGAAGAGGAGGAGGAGGAGGAGGAAGGGGCTTGGAGCGACTACG
GGGGGATGCGGAGAAGCAGTCAGTTCCCTGCACCCAGCACCTCACAGCCCTTCCTCCGTGCGCCCTGCC
GGGCGGCGAGCTAGGCGGCAGCGGCGCGGCGCGGGCTCGGCGGAGCGGCCCATGTCCGGCGCGGGCGAA
GCCCTCGCTCCCGGGCCCGTGGGGCCGCAGCGCGTGGCCGAGGCGGGCGGCGGCCAGCTGGGCTCCACA
GCCCAGGGAAAATGTGATAAAGACAATACTGAGAAAGATATAACTCAAGCTACCAATAGCCACTTCACA
CATGGAGAGATGCAAGACCAGTCCATTTGGGGAAATCCTTCGGATGGTGAACTCATTAGAACCCAACCT
CAGCGCTTGCCTCAGCTTCAGACTTCAGCCCAGGTGCCAAGTGGTGAGGAAATAGGCAAGATAAAGAAC
GGCCACACAGGTCTGAGCAATGGAAATGGAATTCACCACGGGGCCAAACACGGATCCGCAGATAATCGC
AAACTTTCAGCACCTGTTTCTCAAAAAATGCATAGAAAAATTCAGTCCAGCTTGTCTGTAAACAGCGAT
ATCAGTAAGAAGAGCAAAGTAAATGCTGTCTTTTCCCAAAAGACAGGCTCTTCACCTGAAGATTGTTGT
GTCCACTGTATCCTGGCTTGCTTGTTCTGCGAATTCCTGACCCTTTGCAACATTGTCCTGGGACAAGCG
TCATGTGGCATCTGCACCTCAGAAGCCTGCTGCTGTTGCTGTGGTGACGAGATGGGGGATGATTGTAAC
TGCCCTTGTGATATGGACTGTGGCATCATGGATGCCTGTTGTGAATCATCAGACTGCTTGGAAATCTGT
ATGGAATGCTGTGGAATTTGTTTTCCTTCA
ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Protein Sequence
>Peptide sequence encoded by RC211395
Blue=ORF Red=Cloning site Green=Tag(s)

VRGVRAATAAAVAATAASGLSRREAGGRAGAAAAVVRPPGRKCGRCRRLANFPGRKRRRRRRKGLGATT
GGCGEAVSSLHPAPHSPSSVRPAGRRARRQRRGAGSAERPMSGAGEALAPGPVGPQRVAEAGGGQLGST
AQGKCDKDNTEKDITQATNSHFTHGEMQDQSIWGNPSDGELIRTQPQRLPQLQTSAQVPSGEEIGKIKN
GHTGLSNGNGIHHGAKHGSADNRKLSAPVSQKMHRKIQSSLSVNSDISKKSKVNAVFSQKTGSSPEDCC
VHCILACLFCEFLTLCNIVLGQASCGICTSEACCCCCGDEMGDDCNCPCDMDCGIMDACCESSDCLEIC
MECCGICFPS
TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_199072
ORF Size 1065 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_199072.5
RefSeq Size 4602 bp
RefSeq ORF 1068 bp
Locus ID 29969
UniProt ID Q9P1T7
Cytogenetics 7q31.1-q31.2
MW 37 kDa
Summary This gene product is a member of a family of proteins characterized by a specific cysteine-rich C-terminal domain, which is involved in transcriptional regulation of viral genome expression. Alternative translation initiation from an upstream non-AUG (GUG), and an in-frame, downstream AUG codon, results in the production of two isoforms, p40 and p32, respectively, which have different subcellular localization; p32 is mainly found in the cytoplasm, whereas p40 is targeted to the nucleolus. Both isoforms have transcriptional regulatory activity that is attributable to the cysteine-rich C-terminal domain. Alternative splicing results in multiple transcript variants. provided by RefSeq, Oct 2009
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"NM_199072" in other vectors (4)
SKU Description Size Price
RC211395L3 Lenti ORF clone of Human MyoD family inhibitor domain containing (MDFIC), transcript variant 1, Myc-DDK-tagged 10 ug
$757.00
RC211395L4 Lenti ORF clone of Human MyoD family inhibitor domain containing (MDFIC), transcript variant 1, mGFP tagged 10 ug
$757.00
RG211395 MDFIC (tGFP-tagged) - Human MyoD family inhibitor domain containing (MDFIC), transcript variant 1 10 ug
$489.00 $657.00
SC313109 MDFIC (untagged)-Human MyoD family inhibitor domain containing (MDFIC), transcript variant 1 10 ug
$503.00

Plasmid Map for RC211395

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC211395
Circular map for RC211395

file-alt Citations

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