NAP1L5 (NM_153757) Human Tagged ORF Clone

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SKU
RC211191
NAP1L5 (Myc-DDK-tagged)-Human nucleosome assembly protein 1-like 5 (NAP1L5)
  $300.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol NAP1L5
Synonyms DRLM
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC211191 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCGACTCGGAAAACCAGGGGCCTGCGGAGCCTAGCCAGGCGGCGGCAGCGGCGGAGGCAGCGGCGG
AGGAGGTAATGGCGGAAGGCGGTGCGCAGGGTGGAGACTGTGACAGCGCGGCTGGTGACCCTGACAGCGC
GGCTGGTCAGATGGCTGAGGAGCCCCAGACCCCTGCAGAGAATGCCCCAAAGCCGAAAAATGACTTTATC
GAGAGCCTGCCTAATTCGGTGAAATGCCGAGTCCTGGCCCTCAAAAAGCTGCAGAAGCGATGCGATAAGA
TAGAAGCCAAATTTGATAAGGAATTTCAGGCTCTGGAAAAAAAGTATAATGACATCTATAAGCCCCTACT
CGCCAAGATCCAAGAGCTCACCGGCGAGATGGAGGGGTGTGCATGGACCTTGGAGGGGGAGGAGGAGGAG
GAAGAGGAGTACGAGGATGACGAGGAGGAGGGGGAAGACGAGGAGGAGGAGGAGGCTGCGGCAGAGGCTG
CCGCGGGGGCCAAACATGACGATGCCCACGCCGAGATGCCTGATGACGCCAAGAAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC211191 protein sequence
Red=Cloning site Green=Tags(s)

MADSENQGPAEPSQAAAAAEAAAEEVMAEGGAQGGDCDSAAGDPDSAAGQMAEEPQTPAENAPKPKNDFI
ESLPNSVKCRVLALKKLQKRCDKIEAKFDKEFQALEKKYNDIYKPLLAKIQELTGEMEGCAWTLEGEEEE
EEEYEDDEEEGEDEEEEEAAAEAAAGAKHDDAHAEMPDDAKK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_153757
ORF Size 546 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_153757.1, NP_715638.1
RefSeq Size 1965 bp
RefSeq ORF 549 bp
Locus ID 266812
UniProt ID Q96NT1
Cytogenetics 4q21-q22
MW 19.6 kDa
Summary This gene encodes a protein that shares sequence similarity to nucleosome assembly factors, but may be localized to the cytoplasm rather than the nucleus. Expression of this gene is downregulated in hepatocellular carcinomas. This gene is located within a differentially methylated region (DMR) and is imprinted and paternally expressed. There is a related pseudogene on chromosome 4. provided by RefSeq, Nov 2015
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Documents
Resources
"NM_153757" in other vectors (4)
SKU Description Size Price
RC211191L3 Lenti ORF clone of Human nucleosome assembly protein 1-like 5 (NAP1L5), Myc-DDK-tagged 10 ug
$600.00
RC211191L4 Lenti ORF clone of Human nucleosome assembly protein 1-like 5 (NAP1L5), mGFP tagged 10 ug
$600.00
RG211191 NAP1L5 (tGFP-tagged) - Human nucleosome assembly protein 1-like 5 (NAP1L5) 10 ug
$489.00 $500.00
SC100873 NAP1L5 (untagged)-Human nucleosome assembly protein 1-like 5 (NAP1L5) 10 ug
$300.00

Plasmid Map for RC211191

Download sequence for

Cloning scheme for RC211191

Circular map for RC211191

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