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OR2S2 (NM_019897) Human Tagged ORF Clone

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SKU
RC211185
OR2S2 (Myc-DDK-tagged)-Human olfactory receptor, family 2, subfamily S, member 2 (OR2S2)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $300.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol OR2S2
Synonyms OR37A; OST715
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC211185 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAAAAAGCCAATGAGACCTCCCCTGTGATGGGGTTCGTTCTCCTGAGGCTCTCTGCCCACCCAGAGC
TGGAAAAGACATTCTTCGTGCTCATCCTGCTGATGTACCTCGTGATCCTGCTGGGCAATGGGGTCCTCAT
CCTGGTGACCATCCTTGACTCCCGCCTGCACACGCCCATGTACTTCTTCCTAGGGAACCTCTCCTTCCTG
GACATCTGCTTCACTACCTCCTCAGTCCCACTGGTCCTGGACAGCTTTTTGACTCCCCAGGAAACCATCT
CCTTCTCAGCCTGTGCTGTGCAGATGGCACTCTCCTTTGCCATGGCAGGAACAGAGTGCTTGCTCCTGAG
CATGATGGCATTTGATCGCTATGTGGCCATCTGCAACCCCCTTAGGTACTCCGTGATCATGAGCAAGGCT
GCCTACATGCCCATGGCTGCCAGCTCCTGGGCTATTGGTGGTGCTGCTTCCGTGGTACACACATCCTTGG
CAATTCAGCTGCCCTTCTGTGGAGACAATGTCATCAACCACTTCACCTGTGAGATTCTGGCTGTTCTAAA
GTTGGCCTGTGCTGACATTTCCATCAATGTGATCAGCATGGAGGTGACGAATGTGATCTTCCTAGGAGTC
CCGGTTCTGTTCATCTCTTTCTCCTATGTCTTCATCATCACCACCATCCTGAGGATCCCCTCAGCTGAGG
GGAGGAAAAAGGTCTTCTCCACCTGCTCTGCCCACCTCACCGTGGTGATCGTCTTCTACGGGACCTTATT
CTTCATGTATGGGAAGCCTAAGTCTAAGGACTCCATGGGAGCAGACAAAGAGGATCTTTCAGACAAACTC
ATCCCCCTTTTCTATGGGGTGGTGACCCCGATGCTCAACCCCATCATCTATAGCCTGAGGAACAAGGATG
TGAAGGCTGCTGTGAGGAGACTGCTGAGACCAAAAGGCTTCACTCAG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC211185 protein sequence
Red=Cloning site Green=Tags(s)

MEKANETSPVMGFVLLRLSAHPELEKTFFVLILLMYLVILLGNGVLILVTILDSRLHTPMYFFLGNLSFL
DICFTTSSVPLVLDSFLTPQETISFSACAVQMALSFAMAGTECLLLSMMAFDRYVAICNPLRYSVIMSKA
AYMPMAASSWAIGGAASVVHTSLAIQLPFCGDNVINHFTCEILAVLKLACADISINVISMEVTNVIFLGV
PVLFISFSYVFIITTILRIPSAEGRKKVFSTCSAHLTVVIVFYGTLFFMYGKPKSKDSMGADKEDLSDKL
IPLFYGVVTPMLNPIIYSLRNKDVKAAVRRLLRPKGFTQ

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_019897
ORF Size 957 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_019897.2, NP_063950.2
RefSeq Size 1047 bp
RefSeq ORF 960 bp
Locus ID 56656
UniProt ID Q9NQN1
Cytogenetics 9p13.3
Protein Families Druggable Genome, Transmembrane
Protein Pathways Olfactory transduction
MW 35.2 kDa
Summary Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. This olfactory receptor gene is a segregating pseudogene, where some individuals have an allele that encodes a functional olfactory receptor, while other individuals have an allele encoding a protein that is predicted to be non-functional. provided by RefSeq, Jul 2015
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Documents
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"NM_019897" in other vectors (4)
SKU Description Size Price
RC211185L3 Lenti ORF clone of Human olfactory receptor, family 2, subfamily S, member 2 (OR2S2), Myc-DDK-tagged 10 ug
$600.00
RC211185L4 Lenti ORF clone of Human olfactory receptor, family 2, subfamily S, member 2 (OR2S2), mGFP tagged 10 ug
$600.00
RG211185 OR2S2 (tGFP-tagged) - Human olfactory receptor, family 2, subfamily S, member 2 (OR2S2) 10 ug
$489.00 $500.00
SC317485 OR2S2 (untagged)-Human olfactory receptor, family 2, subfamily S, member 2 (OR2S2) 10 ug
$330.00

Plasmid Map for RC211185

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC211185
Circular map for RC211185

file-alt Citations

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