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PDE10A (NM_006661) Human Tagged ORF Clone

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SKU
RC211181
PDE10A (Myc-DDK-tagged)-Human phosphodiesterase 10A (PDE10A), transcript variant 2
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $713.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol PDE10A
Synonyms ADSD2; HSPDE10A; IOLOD; LINC00473; PDE10A19
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC211181 representing NM_006661
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGGATAGAAGAGAGGAAATCCCAACATTTAACAGGTTTGACAGATGAAAAAGTGAAGGCATATCTTT
CTCTTCACCCCCAGGTATTAGATGAATTTGTATCTGAAAGTGTTAGTGCAGAGACAGTAGAGAAATGGCT
GAAGAGGAAGAACAACAAATCAGAAGATGAATCAGCTCCTAAGGAAGTCAGCAGGTACCAAGATACGAAT
ATGCAGGGAGTTGTATATGAACTAAACAGCTATATAGAACAACGGTTGGACACAGGAGGAGACAACCAGC
TACTCCTCTATGAACTGAGCAGCATCATTAAAATAGCCACAAAAGCCGATGGATTTGCACTGTATTTCCT
TGGAGAGTGCAATAATAGCCTGTGTATATTCACGCCACCTGGGATAAAGGAAGGAAAACCCCGCCTCATC
CCTGCTGGGCCCATCACTCAGGGCACCACCGTCTCTGCTTATGTGGCCAAGTCCAGGAAAACACTGCTAG
TAGAAGACATCCTTGGAGATGAACGATTTCCAAGAGGTACTGGACTGGAATCAGGGACTCGTATCCAGTC
TGTTCTTTGCTTACCAATTGTCACTGCAATTGGTGACTTGATTGGTATTCTCGAGCTGTATCGGCACTGG
GGCAAAGAAGCCTTCTGTCTTAGTCACCAGGAGGTTGCAACAGCAAATCTTGCCTGGGCTTCAGTAGCAA
TACATCAGGTGCAGGTATGCAGAGGCCTTGCCAAACAGACAGAATTGAATGACTTCCTACTCGACGTATC
AAAAACATATTTTGATAACATAGTTGCAATAGATTCTCTACTTGAACACATAATGATATATGCAAAAAAC
CTGGTGAATGCCGATCGTTGTGCACTTTTCCAGGTGGACCATAAGAACAAGGAGTTATATTCAGACCTTT
TTGATATTGGAGAGGAAAAGGAAGGAAAACCTGTCTTCAAGAAGACCAAAGAGATAAGATTTTCAATTGA
GAAAGGAATTGCTGGCCAAGTAGCAAGAACAGGGGAAGTCCTGAACATTCCAGATGCCTATGCAGACCCA
CGCTTTAACAGAGAAGTAGACTTGTACACAGGCTACACCACGCGGAACATCCTGTGCATGCCCATCGTCA
GCCGAGGCAGCGTGATAGGTGTGGTGCAGATGGTCAACAAAATCAGTGGCAGTGCCTTCTCTAAAACAGA
TGAAAACAACTTCAAAATGTTTGCCGTCTTTTGTGCTTTAGCCTTACACTGTGCTAATATGTATCATAGA
ATTCGCCACTCAGAGTGCATTTACCGGGTAACGATGGAAAAGCTGTCCTACCATAGCATTTGTACTTCAG
AAGAGTGGCAAGGTCTCATGCAATTCACCCTTCCCGTGCGTCTCTGCAAAGAAATTGAATTATTCCACTT
TGACATTGGTCCTTTTGAAAACATGTGGCCTGGAATTTTTGTCTACATGGTTCATCGGTCCTGTGGGACA
TCCTGCTTTGAGCTTGAAAAGTTGTGTCGTTTTATTATGTCTGTGAAGAAGAACTATCGGCGGGTTCCTT
ATCACAACTGGAAGCATGCGGTCACTGTAGCACACTGCATGTATGCCATACTTCAGAACAATCACACGCT
TTTCACAGACCTTGAGCGCAAAGGACTGCTGATTGCGTGTCTGTGTCATGACCTGGACCACAGGGGCTTC
AGTAACAGCTACCTGCAGAAGTTCGACCACCCTCTGGCCGCTCTCTACTCCACTTCCACCATGGAGCAGC
ACCACTTCTCCCAGACTGTGTCCATCCTTCAGTTGGAAGGGCACAATATCTTCTCCACTCTGAGCTCCAG
TGAATATGAGCAGGTGCTTGAGATCATCCGCAAAGCCATCATTGCCACAGACCTTGCTTTATACTTTGGA
AACAGGAAGCAGTTGGAAGAGATGTACCAGACCGGATCACTAAACCTTAATAATCAATCACATAGAGACC
GTGTAATTGGTTTGATGATGACTGCCTGTGACCTTTGTTCTGTGACAAAACTGTGGCCCGTTACAAAATT
GACGGCAAATGATATATATGCAGAATTCTGGGCTGAGGGTGATGAAATGAAGAAATTGGGAATACAGCCT
ATTCCTATGATGGACAGAGACAAGAAGGATGAAGTCCCCCAAGGCCAGCTTGGGTTCTACAATGCCGTGG
CCATTCCCTGCTATACAACCCTTACCCAGATCCTCCCTCCCACGGAGCCTCTTCTGAAAGCATGCAGGGA
TAATCTCAGTCAGTGGGAGAAGGTGATTCGAGGGGAGGAGACTGCAACCTGGATTTCATCCCCATCCGTG
GCTCAGAAGGCAGCTGCATCTGAAGAT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC211181 representing NM_006661
Red=Cloning site Green=Tags(s)

MRIEERKSQHLTGLTDEKVKAYLSLHPQVLDEFVSESVSAETVEKWLKRKNNKSEDESAPKEVSRYQDTN
MQGVVYELNSYIEQRLDTGGDNQLLLYELSSIIKIATKADGFALYFLGECNNSLCIFTPPGIKEGKPRLI
PAGPITQGTTVSAYVAKSRKTLLVEDILGDERFPRGTGLESGTRIQSVLCLPIVTAIGDLIGILELYRHW
GKEAFCLSHQEVATANLAWASVAIHQVQVCRGLAKQTELNDFLLDVSKTYFDNIVAIDSLLEHIMIYAKN
LVNADRCALFQVDHKNKELYSDLFDIGEEKEGKPVFKKTKEIRFSIEKGIAGQVARTGEVLNIPDAYADP
RFNREVDLYTGYTTRNILCMPIVSRGSVIGVVQMVNKISGSAFSKTDENNFKMFAVFCALALHCANMYHR
IRHSECIYRVTMEKLSYHSICTSEEWQGLMQFTLPVRLCKEIELFHFDIGPFENMWPGIFVYMVHRSCGT
SCFELEKLCRFIMSVKKNYRRVPYHNWKHAVTVAHCMYAILQNNHTLFTDLERKGLLIACLCHDLDHRGF
SNSYLQKFDHPLAALYSTSTMEQHHFSQTVSILQLEGHNIFSTLSSSEYEQVLEIIRKAIIATDLALYFG
NRKQLEEMYQTGSLNLNNQSHRDRVIGLMMTACDLCSVTKLWPVTKLTANDIYAEFWAEGDEMKKLGIQP
IPMMDRDKKDEVPQGQLGFYNAVAIPCYTTLTQILPPTEPLLKACRDNLSQWEKVIRGEETATWISSPSV
AQKAAASED

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_006661
ORF Size 2337 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_006661.4
RefSeq Size 4576 bp
RefSeq ORF 2340 bp
Locus ID 10846
UniProt ID Q9Y233, A0A1B1UZR0
Cytogenetics 6q27
Domains GAF, PDEase
Protein Families Druggable Genome
Protein Pathways Progesterone-mediated oocyte maturation, Purine metabolism
MW 88.2 kDa
Summary The protein encoded by this gene belongs to the cyclic nucleotide phosphodiesterase family. It plays a role in signal transduction by regulating the intracellular concentration of cyclic nucleotides. This protein can hydrolyze both cAMP and cGMP to the corresponding nucleoside 5' monophosphate, but has higher affinity for cAMP, and is more efficient with cAMP as substrate. Alternatively spliced transcript variants have been described for this gene. provided by RefSeq, Dec 2011
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Documents
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"NM_006661" in other vectors (6)
SKU Description Size Price
RC211181L1 Lenti ORF clone of Human phosphodiesterase 10A (PDE10A), transcript variant 2, Myc-DDK-tagged 10 ug
$1,013.00
RC211181L2 Lenti ORF clone of Human phosphodiesterase 10A (PDE10A), transcript variant 2, mGFP tagged 10 ug
$1,013.00
RC211181L3 Lenti ORF clone of Human phosphodiesterase 10A (PDE10A), transcript variant 2, Myc-DDK-tagged 10 ug
$1,013.00
RC211181L4 Lenti ORF clone of Human phosphodiesterase 10A (PDE10A), transcript variant 2, mGFP tagged 10 ug
$1,013.00
RG211181 PDE10A (tGFP-tagged) - Human phosphodiesterase 10A (PDE10A), transcript variant 2 10 ug
$789.00 $913.00
SC115943 PDE10A (untagged)-Human phosphodiesterase 10A (PDE10A), transcript variant 2 10 ug
$714.00

Plasmid Map for RC211181

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC211181
Circular map for RC211181

file-alt Citations

Loss of PDE10A is associated with metastasis of colon adenocarcinoma
Arowa, Nuechterlein, Ha et al
Histol Histopathol (2025)
AI Summary: In this study, the Human PDE10A (Myc-DDK-tagged) ORF Clone (catalog #RC211181) from OriGene was used to investigate the role of PDE10A loss in colon adenocarcinoma metastasis and its association with poor clinical outcomes.
Snippet: Human PDE10A (Myc-DDK-tagged) ORF Clone (NM_006661), transcript variant 2 cloned into the pCMV6-Entry vector was obtained from Origene (catalog #RC211181)
Showing 1-1 of 1 papers.
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