FLJ12331 (NM_024986) Human Tagged ORF Clone

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SKU
RC211166
FLJ12331 (Myc-DDK-tagged)-Human hypothetical protein FLJ12331 (FLJ12331)
  $150.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol FLJ12331
Synonyms MGC132479, MGC132481
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC211166 representing NM_024986
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCGGAGAGGTTACTGGGGGAACAAGATCGGCAAGTCCTACACCACCCCCTGTATGGTTACAGGCTGCT
GTAGCTCTGGGCTAGTACACCTTATCCCTGCCCCCAGGAACAATGGCATCATCTCGACTCCTGTGCCCAA
GATGCTGCTGCTGATGGCCGGTATTGACAACTGCTACACCTCAGCCAGGGGCTGCACTGCCACCCTGGAC
AACTTCACCAAGGCCACCTTCAATGCCATCTCCAGGACCTATAGCTGCCTGACCCCCAGCCTCTGGAAAG
AGACTATTCACCAAGACTCCCTGTGGAATGCACTGACCATCACATCAAGACCCACACCAGAGCCTCCATG
CAGAGGACCCAGGCTCCAGCTGTGGTTACAGCATAAGCTTTCATACAAGAATAATAGTGAATTACACCTG
TTAAAAATAATAAGCAGAAGAAAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC211166 representing NM_024986
Red=Cloning site Green=Tags(s)

MRRGYWGNKIGKSYTTPCMVTGCCSSGLVHLIPAPRNNGIISTPVPKMLLLMAGIDNCYTSARGCTATLD
NFTKATFNAISRTYSCLTPSLWKETIHQDSLWNALTITSRPTPEPPCRGPRLQLWLQHKLSYKNNSELHL
LKIISRRK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_024986
ORF Size 444 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_024986.1, NP_079262.1
RefSeq Size 2057 bp
RefSeq ORF 446 bp
Locus ID 80052
Cytogenetics 16q22.1
MW 17 kDa
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Documents
Resources
"NM_024986" in other vectors (3)
SKU Description Size Price
RC211166L3 Lenti ORF clone of Human hypothetical protein FLJ12331 (FLJ12331), Myc-DDK-tagged 10 ug
$450.00
RC211166L4 Lenti ORF clone of Human hypothetical protein FLJ12331 (FLJ12331), mGFP tagged 10 ug
$450.00
RG211166 FLJ12331 (tGFP-tagged) - Human hypothetical protein FLJ12331 (FLJ12331) 10 ug
$489.00

Plasmid Map for RC211166

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Cloning scheme for RC211166

Circular map for RC211166

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