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CDC2L2 (NM_033532) Human Tagged ORF Clone

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SKU
RC211124
CDC2L2 (Myc-DDK-tagged)-Human cell division cycle 2-like 2 (PITSLRE proteins) (CDC2L2), transcript variant 6
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $457.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol CDC2L2
Synonyms CDC2L3, p58GTA, PITSLRE, CDK11-p46, CDK11-p58, MGC131975, CDK11-p110
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC211124 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAAGAACGAGAAAATGAAAACCACCTCTTGGTTGTTCCAGAGTCACGGTTCGACCGAGATTCCGGGGA
GAGTGAAGAAGCAGAGGAAGAAGTGGGGCTGCCGGAGCGTCGAGGAGTTCCAGTGCCTGAACAGGATCGA
GGAGGGCACCTATGGAGTGGTCTACAGAGCAAAAGACAAGAAAACAGATGAAATTGTGGCTCTAAAGCGG
CTGAAGATGGAGAAGGAGAAGGAGGGCTTCCCGATCACGTCCCTGAGGGAGATCAACACCATCCTCAAGG
CCCAGCATCCCAACATTGTCACCGTTAGAGAGATTGTGGTGGGCAGCAACATGGACAAGATCTACATCGT
GATGAACTATGTGGAGCACGACCTCAAGAGCCTGATGGAGACCATGAAACAGCCCTTCCTGCCAGGGGAG
GTGAAGACCCTGATGATCCAGCTGCTGCGTGGGGTGAAACACCTGCACGACAACTGGATCCTGCACCGTG
ACCTCAAGACGTCCAACCTGCTGCTGAGCCACGCCGGCATCCTCAAGGTGGGTGATTTTGGGCTGGCGCG
GGAGTACGGATCCCCTCTGAAGGCCTACACCCCGGTCGTGGTGACCCAGTGGTACCGCGCCCCAGAGCTG
CTGCTTGGTGCCAAGGAATACTCCACGGCCGTGGACATGTGGTCAGTGGGCTGCATCTTCGGGGAGCTGC
TGACTCAGAAGCCTCTGTTCCCCGGGAATTCGGAAATCGATCAGATCAACAAAGTGTTCAAGGAGCTGGG
GACCCCCAGTGAGAAAATCTGGCCCGGCTACAGTGAGCTCCCAGTAGTCAAGAAGATGACCTTCAGCGAG
CACCCCTACAACAACCTCCGCAAGCGCTTCGGGGCTCTGCTCTCAGACCAGGGCTTCGACCTCATGAACA
AGTTCCTGACCTACTTCCCCGGGAGGAGGATCAGCGCTGAGGACGGCCTCAAGCATGAGTATTTCCGCGA
GACCCCCCTCCCCATCGACCCCTCCATGTTCCCCACGTGGCCCGCCAAGAGCGAGCAGCAGCGTGTGAAG
CGGGGCACCAGCCCGAGGCCCCCTGAGGGAGGCCTGGGCTACAGCCAGCTGGGTGACGACGACCTGAAGG
AGACGGGCTTCCACCTTACCACCACGAACCAGGGGGCCTCTGCCGCGGGCCCCGGCTTCAGCCTCAAGTT
C

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC211124 protein sequence
Red=Cloning site Green=Tags(s)

MKNEKMKTTSWLFQSHGSTEIPGRVKKQRKKWGCRSVEEFQCLNRIEEGTYGVVYRAKDKKTDEIVALKR
LKMEKEKEGFPITSLREINTILKAQHPNIVTVREIVVGSNMDKIYIVMNYVEHDLKSLMETMKQPFLPGE
VKTLMIQLLRGVKHLHDNWILHRDLKTSNLLLSHAGILKVGDFGLAREYGSPLKAYTPVVVTQWYRAPEL
LLGAKEYSTAVDMWSVGCIFGELLTQKPLFPGNSEIDQINKVFKELGTPSEKIWPGYSELPVVKKMTFSE
HPYNNLRKRFGALLSDQGFDLMNKFLTYFPGRRISAEDGLKHEYFRETPLPIDPSMFPTWPAKSEQQRVK
RGTSPRPPEGGLGYSQLGDDDLKETGFHLTTTNQGASAAGPGFSLKF

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_033532
ORF Size 1191 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_033532.1, NP_277074.1
RefSeq Size 2362 bp
RefSeq ORF 1193 bp
Locus ID 985
Cytogenetics 1p36.33
Protein Families Protein Kinase, Transcription Factors
MW 45.2 kDa
Summary This gene encodes a member of the p34Cdc2 protein kinase family. p34Cdc2 kinase family members are known to be essential for eukaryotic cell cycle control. This gene is in close proximity to CDC2L1, a nearly identical gene in the same chromosomal region. The gene loci including this gene, CDC2L1, as well as metalloprotease MMP21/22, consist of two identical, tandemly linked genomic regions, which are thought to be a part of the larger region that has been duplicated. This gene and CDC2L1 were shown to be deleted or altered frequently in neuroblastoma with amplified MYCN genes. The protein kinase encoded by this gene could be cleaved by caspases and was demonstrated to play roles in cell apoptosis. Many transcript variants encoding several different isoforms have been found for this gene, but the full-length nature of only two have been determined so far.
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"NM_033532" in other vectors (5)
SKU Description Size Price
RC211124L1 Lenti ORF clone of Human cell division cycle 2-like 2 (PITSLRE proteins) (CDC2L2), transcript variant 6, Myc-DDK-tagged 10 ug
$757.00
RC211124L2 Lenti ORF clone of Human cell division cycle 2-like 2 (PITSLRE proteins) (CDC2L2), transcript variant 6, mGFP tagged 10 ug
$757.00
RC211124L3 Lenti ORF clone of Human cell division cycle 2-like 2 (PITSLRE proteins) (CDC2L2), transcript variant 6, Myc-DDK-tagged 10 ug
$757.00
RC211124L4 Lenti ORF clone of Human cell division cycle 2-like 2 (PITSLRE proteins) (CDC2L2), transcript variant 6, mGFP tagged 10 ug
$757.00
RG211124 CDC2L2 (tGFP-tagged) - Human cell division cycle 2-like 2 (PITSLRE proteins) (CDC2L2), transcript variant 6 10 ug
$489.00 $657.00

Plasmid Map for RC211124

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC211124
Circular map for RC211124

file-alt Citations

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