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PPP2R5A (NM_006243) Human Tagged ORF Clone

  • TrueORF®
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SKU
RC211104
PPP2R5A (Myc-DDK-tagged)-Human protein phosphatase 2, regulatory subunit B', alpha (PPP2R5A), transcript variant 1
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $457.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "PPP2R5A or NM_006243"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol PPP2R5A
Synonyms B56A; B56alpha; PR61A
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC211104 representing NM_006243
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCGTCGTCGTCGCCGCCGGCGGGGGCTGCCAGCGCCGCCATCTCGGCCTCGGAGAAAGTGGACGGCT
TCACCCGGAAATCGGTCCGCAAGGCGCAGAGGCAGAAGCGCTCCCAGGGCTCGTCGCAGTTTCGCAGCCA
GGGCAGCCAGGCAGAGCTGCACCCGCTGCCCCAGCTCAAAGATGCCACTTCAAATGAACAACAAGAGCTT
TTCTGTCAGAAGTTGCAGCAGTGTTGTATACTGTTTGATTTCATGGACTCTGTTTCAGACTTGAAGAGCA
AAGAAATTAAAAGAGCAACACTGAATGAACTGGTTGAGTATGTTTCAACTAATCGTGGTGTAATTGTTGA
ATCAGCGTATTCTGATATAGTAAAAATGATCAGTGCTAACATCTTCCGTACACTTCCTCCAAGTGATAAT
CCAGATTTTGATCCAGAAGAGGATGAACCCACGCTTGAGGCCTCTTGGCCTCACATACAGTTGGTATATG
AATTCTTCTTGAGATTTTTGGAGAGCCCTGATTTCCAGCCTAGCATTGCAAAACGATACATTGATCAGAA
ATTCGTACAACAGCTCCTGGAGCTTTTTGATAGTGAAGATCCCAGAGAACGTGACTTCCTGAAGACTGTT
CTGCACCGAATTTATGGGAAATTTCTTGGATTAAGAGCATTCATCAGAAAACAAATTAACAACATTTTCC
TCAGGTTTATATATGAAACAGAACATTTCAATGGTGTTGCTGAACTTCTTGAAATATTAGGAAGTATTAT
CAATGGCTTTGCATTGCCACTGAAAGCAGAACATAAACAATTTCTAATGAAGGTTCTTATTCCTATGCAT
ACTGCAAAAGGATTAGCTTTGTTTCATGCTCAGCTAGCATATTGTGTTGTACAGTTCCTGGAGAAAGATA
CAACACTAACAGAGCCAGTGATCAGAGGACTGCTGAAATTTTGGCCAAAAACCTGCAGTCAGAAAGAGGT
GATGTTTTTAGGAGAAATTGAAGAAATCTTAGATGTCATTGAACCAACACAGTTCAAAAAAATTGAAGAG
CCACTTTTCAAGCAGATATCCAAGTGTGTATCCAGTTCTCATTTTCAGGTTGCAGAAAGGGCATTGTACT
TCTGGAATAACGAATATATTCTTAGTTTGATTGAGGAGAACATTGATAAAATTCTGCCAATTATGTTTGC
CAGTTTGTACAAAATTTCCAAAGAACACTGGAATCCGACCATTGTAGCACTGGTATACAATGTGCTGAAA
ACCCTAATGGAAATGAATGGCAAGCTTTTCGATGACCTTACTAGCTCATACAAAGCTGAAAGACAGAGAG
AGAAAAAGAAGGAATTGGAACGTGAAGAATTATGGAAAAAATTAGAGGAGCTAAAGCTAAAGAAAGCTCT
AGAAAAACAGAATAGTGCTTACAACATGCACAGTATTCTCAGCAATACAAGTGCCGAA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC211104 representing NM_006243
Red=Cloning site Green=Tags(s)

MSSSSPPAGAASAAISASEKVDGFTRKSVRKAQRQKRSQGSSQFRSQGSQAELHPLPQLKDATSNEQQEL
FCQKLQQCCILFDFMDSVSDLKSKEIKRATLNELVEYVSTNRGVIVESAYSDIVKMISANIFRTLPPSDN
PDFDPEEDEPTLEASWPHIQLVYEFFLRFLESPDFQPSIAKRYIDQKFVQQLLELFDSEDPRERDFLKTV
LHRIYGKFLGLRAFIRKQINNIFLRFIYETEHFNGVAELLEILGSIINGFALPLKAEHKQFLMKVLIPMH
TAKGLALFHAQLAYCVVQFLEKDTTLTEPVIRGLLKFWPKTCSQKEVMFLGEIEEILDVIEPTQFKKIEE
PLFKQISKCVSSSHFQVAERALYFWNNEYILSLIEENIDKILPIMFASLYKISKEHWNPTIVALVYNVLK
TLMEMNGKLFDDLTSSYKAERQREKKKELEREELWKKLEELKLKKALEKQNSAYNMHSILSNTSAE

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_006243
ORF Size 1458 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_006243.4
RefSeq Size 3145 bp
RefSeq ORF 1461 bp
Locus ID 5525
UniProt ID Q15172
Cytogenetics 1q32.3
Domains B56
Protein Families Druggable Genome, Phosphatase
Protein Pathways Oocyte meiosis, Wnt signaling pathway
MW 56 kDa
Summary The product of this gene belongs to the phosphatase 2A regulatory subunit B family. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The B regulatory subunit might modulate substrate selectivity and catalytic activity. This gene encodes an alpha isoform of the regulatory subunit B56 subfamily. Alternative transcript variants encoding distinct isoforms have been found for this gene. provided by RefSeq, Dec 2010
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"NM_006243" in other vectors (6)
SKU Description Size Price
RC211104L1 Lenti-ORF clone of PPP2R5A (Myc-DDK-tagged)-Human protein phosphatase 2, regulatory subunit B', alpha (PPP2R5A), transcript variant 1 10 ug
$757.00
RC211104L2 Lenti-ORF clone of PPP2R5A (mGFP-tagged)-Human protein phosphatase 2, regulatory subunit B', alpha (PPP2R5A), transcript variant 1 10 ug
$757.00
RC211104L3 Lenti-ORF clone of PPP2R5A (Myc-DDK-tagged)-Human protein phosphatase 2, regulatory subunit B', alpha (PPP2R5A), transcript variant 1 10 ug
$757.00
RC211104L4 Lenti-ORF clone of PPP2R5A (mGFP-tagged)-Human protein phosphatase 2, regulatory subunit B', alpha (PPP2R5A), transcript variant 1 10 ug
$757.00
RG211104 PPP2R5A (tGFP-tagged) - Human protein phosphatase 2, regulatory subunit B', alpha (PPP2R5A), transcript variant 1 10 ug
$489.00 $657.00
SC116240 PPP2R5A (untagged)-Human protein phosphatase 2, regulatory subunit B', alpha (PPP2R5A), transcript variant 1 10 ug
$457.00

Plasmid Map for RC211104

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC211104
Circular map for RC211104

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